Detection and genetic characterization of porcine circovirus type 2 (PCV2) in pigs from Croatia

Jemeršić, Lorena; Cvetnić, Željko; Toplak, Ivan; Špičić, Silvio; Grom, Jože; Barlič–Maganja, Darja; Terzić, Svjetlana; Hostnik, Peter; Lojkić, Mirko; Humski, Andrea; Habrun, Boris; Krt, B.

doi:10.1016/j.rvsc.2004.04.003

Cited by 15 publications

(9 citation statements)

References 22 publications

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“…Therefore, our strains will be part of one large cluster defined by Larochelle et al (2002), which is composed of PCV2 strains from Europe, Taiwan, China and Canada. The present study confirms previous phylogenetic studies regarding PCV2 in Croatia (Jemeršić et al, 2004). Also, two out of six Hungarian isolates (375 and 304; Dán et al, 2003) were very closely related to those of our study.…”

Section: Discussionsupporting

confidence: 92%

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Postweaning multisystemic wasting syndrome (pmws) in pigs in Croatia: Detection and characterisation of porcine circovirus type 2 (pcv2)

Lipej

Segalés

Toplak³

et al. 2005

Acta Veterinaria Hungarica

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The objective of this study was to characterise porcine circovirus type 2 (PCV2) from pigs with naturally occurring postweaning multisystemic wasting syndrome (PMWS) in Croatia, and to determine the epizootiological, clinical and pathomorphological features of the disease. During a systematic health monitoring programme conducted in the period from January 2002 to June 2003, PMWS was suspected on eight different pig-producing farms in Croatia. The diagnosis of PMWS met all three key criteria: the presence of compatible clinical signs, the presence of the characteristic microscopic lymphoid lesions, and the detection of PCV2 within the lesions by polymerase chain reaction (PCR) and by in situ hybridisation (ISH). Moreover, PCV2 DNA from swine tissues was extracted and sequenced. The phylogenetic analysis of 4 Croatian PCV2 strains showed close relationship to PCV2 strains isolated in Slovenia, France, the Netherlands, the United Kingdom, China and Hungary. PCV2 was also demonstrated by electron microscopy in the lymph node of an affected animal. This is the first demonstration of PMWS in Croatia based on all scientifically accepted diagnostic criteria.

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Section: Discussionsupporting

confidence: 92%

“…Only the representative sequences of PCV2 from Croatian pig farms, generated in this study (Cro 36,50, are presented in bold. The isolates CRO1 and CRO2 were obtained from Jemeršić et al (2004). The sequences HUNG 375 and HUNG 304 represent PCV2 strains from Hungary (Dán et al, 2003).…”

Section: Microbiological Resultsmentioning

confidence: 99%

Postweaning multisystemic wasting syndrome (pmws) in pigs in Croatia: Detection and characterisation of porcine circovirus type 2 (pcv2)

Lipej

Segalés

Toplak³

et al. 2005

Acta Veterinaria Hungarica

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“…Besides domestic pigs (Jemersic et al, 2004), PCV2 antibody has been detected in Croatian wild boars (Lipej et al, 2007). We found PCV2 antibodies (15.1% overall prevalence) in wild boars in all investigated counties.…”

Section: Discussionmentioning

confidence: 61%

Prevalence of Antibodies to Selected Viral Pathogens in Wild Boars (Sus Scrofa) in Croatia in 2005–06 and 2009–10

Roić

Jemeršić

Terzić

et al. 2012

Journal of Wildlife Diseases

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ABSTRACT:We determined prevalence of antibody to selected viral pathogens important for domestic pigs and livestock in 556 wild boar (Sus scrofa) sera collected during 2005-06 and 2009-10 in four counties in Croatia. These counties account for an important part of the Croatian commercial pig production and have a high density of wild boars. Samples were tested for antibodies to porcine parvovirus (PPV), Aujeszky's disease virus (ADV), porcine circovirus type 2 (PCV2), swine influenza virus, porcine respiratory and reproductive syndrome virus (PRRSV), porcine respiratory coronavirus (PRCV), transmissible gastroenteritis virus, and swine vesicular disease virus (SVDV). Antibodies to all of the infectious pathogens except SVDV were detected. There was a statistically significant difference in prevalence between the two periods for PPV, ADV, PCV2, PRRSV, and PRCV, with a higher prevalence of PPV and ADV in the 2009-10 period (P,0.05). During the same period, the prevalence of PCV2, PRRSV, and PRCV was lower (P,0.05). Our results provide information on the current disease exposure and health status of wild boars in Croatia and suggest that wild boars may act as a reservoir for several pathogens and a source of infection for domestic pigs and other livestock as well as humans, especially for ADV.

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“…Both, PRRS [17] and PCV2 [18] infections have been described in Croatia in 1997 and 2004, respectively, on large, small and medium size pig farms in several Croatian counties. Since then, limited data is available on the predominant genotypes and eventual genetic changes of both viruses, especially after introducing vaccination.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic comparison of porcine circovirus type 2 (PCV2) and porcine reproductive respiratory syndrome virus (PRRSV) strains detected in domestic pigs until 2008 and in 2012 in Croatia

et al. 2014

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BackgroundPorcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) have been present for the last 2 decades in Croatia, causing large economical losses in the pig production. The clinical features of the infections are mostly manifested by the development of respiratory problems, weight loss and poor growth performance, as well as reproductive failure in pregnant sows. Even though the infections are continuously recognized in some regions in Croatia, the heterogeneity of the detected viral strains from 2012 has not yet been investigated. The objective of this study was to compare virus strains of PCV2 and PRRSV detected until 2008 in Croatia with strains isolated in 2012 to gain a better epidemiological understanding of these two infections.ResultsPCV2 and PRRSV strains detected in 2012 in fattening pigs from regions where these two diseases have been previously described were compared to strains that have been detected in the same regions within the past two decades. The phylogenetic analysis revealed that the circulating PCV2 and PRRSV strains are distantly related to the previously described Croatian viral strains. However, when compared to known isolates from the GenBank a high genetic identity of PRRSV isolates with isolates from Hungary, Denmark and the Netherlands was found.ConclusionThe results of this study reveal that even though PCV2 and PRRSV are constantly present in the investigated regions in Croatia, the viral strains found in 2012 genetically differ from those detected in earlier years. This indicates that new entries into the pig population appeared with regard to both infections, probably as a result of pig trade.

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Detection and genetic characterization of porcine circovirus type 2 (PCV2) in pigs from Croatia

Cited by 15 publications

References 22 publications

Postweaning multisystemic wasting syndrome (pmws) in pigs in Croatia: Detection and characterisation of porcine circovirus type 2 (pcv2)

Postweaning multisystemic wasting syndrome (pmws) in pigs in Croatia: Detection and characterisation of porcine circovirus type 2 (pcv2)

Prevalence of Antibodies to Selected Viral Pathogens in Wild Boars (Sus Scrofa) in Croatia in 2005–06 and 2009–10

Phylogenetic comparison of porcine circovirus type 2 (PCV2) and porcine reproductive respiratory syndrome virus (PRRSV) strains detected in domestic pigs until 2008 and in 2012 in Croatia

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