Detection and genetic identification of pestiviruses in Brazilian lots of fetal bovine serum collected from 2006 to 2014

Monteiro, Francielle Liz; Cargnelutti, Juliana Felipetto; Bräunig, Patrícia; Folgueras-Flatschart, Áurea Valadares; Santos, Nathália C.; Pituco, Edviges Maristela; Weiblen, Rudi; Flores, Eduardo Furtado

doi:10.1590/1678-5150-pvb-5283

Cited by 16 publications

(35 citation statements)

References 22 publications

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“…Finally, phylogenetic analysis of all 21 HoBiPeV genomes, amplified by 5′ UTR primers N2-R5, grouped them together and in the same genetic group of several previously described Brazilian sequences, subgroup 3a (Fig. 3) [24,27,29,31].…”

Section: Resultsmentioning

confidence: 75%

“…Phylogenetic analysis of BVDV-2 sequences amplified by primers 90-368 [9] and BVDV2#3 [24] grouped them in the BVDV-2b subgenotype (Fig. 2).…”

Section: Resultsmentioning

confidence: 99%

“…HoBiPeV were initially identified as contaminants of fetal bovine serum (FBS) of Brazilian origin [13] and were subsequently linked to several livestock diseases in South America [14,15], Italy [16][17][18], and Asian countries [19][20][21]. BVDV-1 and BVDV-2 subgenotypes have been shown to circulate in Brazilian cattle in varied proportions, yet most genetic studies have been based on a single or few isolates or have included samples scattered over time and from different geographical origins or clinical backgrounds [7,14,[22][23][24][25][26][27][28]. The first evidence of circulation of HoBiPeV in Brazil dates from around 2002 and a number of subsequent studies indicate that these viruses are endemic among Brazilian cattle [7,14,15,24,25,27,[29][30][31].…”

Section: Introductionmentioning

confidence: 99%

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Genetic identification of pestiviruses from beef cattle in Southern Brazil

et al. 2019

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Bovine pestiviruses, e.g., bovine viral diarrhea virus types 1 (BVDV-1 or Pestivirus A), BVDV-2 (Pestivirus B), and HoBi-like pestiviruses (HoBiPeV or Pestivirus H), have been shown to circulate in Brazilian cattle in varied proportions. In this study, we identified genetically pestiviruses circulating in beef cattle in Rio Grande do Sul, the southern most Brazilian state. Screening of serum of 15.584 beef calves destined to be export by an antigen capture ELISA and, subsequently, by reverse-transcription polymerase chain reaction (RT-PCR), revealed 135 containing pestivirus RNA. Genetic typing of these viruses based on nucleotide sequencing and phylogenetic analysis of the 5′ untranslated region (5′ UTR) of the viral genome allowed for the identification of 90 different viruses, being 38 BVDV-1 (42.2%), 31 BVDV-2 (34.4%), and 21 HoBiPeV (23.4%). Among BVDV-1, only subtypes BVDV-1a (n = 28, 31.1%) and BVDV-1b (n = 10, 11.1%) were identified. All 31 BVDV-2 isolates belonged to BVDV-2b subtype and the 21 HoBiPeV viruses clustered to subgroup 3a. Thus, this study provides an approximate genetic profile of pestiviruses circulating in beef cattle in a traditional Brazilian beef cattle-raising state.

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Section: Resultsmentioning

confidence: 75%

“…Phylogenetic analysis of BVDV-2 sequences amplified by primers 90-368 [9] and BVDV2#3 [24] grouped them in the BVDV-2b subgenotype (Fig. 2).…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic identification of pestiviruses from beef cattle in Southern Brazil

et al. 2019

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“…Brazil seems to have an important role on the HoBiPeV description in other countries. In 2004, the HoBiPeV was first identified in Europe, in FBS imported from Brazil (Schirrmeier et al, 2004), and to date, more than one‐third of all HoBiPeV identified in the world have come from FBS, labelled as having a Brazilian or (broadly) South American origin (Bauermann et al, 2013; Jardim et al, 2018; Monteiro et al, 2018; Silveira et al, 2017). This fact could be explained by an increase of the Brazilian bovine population in the 1970s (ABIEC, 2016) and a increasing exportation of Brazilian bovine products to several countries.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic and evolutionary analysis of HoBi‐like pestivirus: Insights into origin and dispersal

Silveira

Cibulski

Junqueira³

et al. 2020

Transbound Emerg Dis

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The HoBi‐like pestivirus (HoBiPeV), currently classified as Pestivirus H species, is a pathogen associated with a broad spectrum of clinical manifestations in ruminants, particularly in cattle. Since HoBiPeV complete genome sequencing data is scarce, in the present study we described five nearly complete new Brazilian HoBiPeV genomes and further perform a more complete genetic and evolutionary characterization with all additional genome sequences available in the GenBank database. Entropy and selection pressure analysis showed the E2 gene, a surface glycoprotein, is the most variable gene, which also displays the greatest number of sites under positive selection. Phylogenetic and Bayesian inference based on complete genome and Npro gene, respectively, from all HoBiPeV sequences available so far, confirms the existence of three main clades (a, b, and c). The abovementioned analysis suggests that this pestivirus species probably emerged in Asia and spread to different regions including Brazil, where only strains belonging to specific genetic group ‘a’ have been found. The hypothesis of the HoBiPeV introduction in Brazil (between 1,890 and 1,962), formulated based on Bayesian inference, coincides with a period of intensive importation of water buffalo (Bubalus arnee) and indicine cattle (Bos taurus indicus) from Asia to Brazil, suggesting that this could be the origin of the current Brazilian HoBiPeV genetic group ‘a’.

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“…Most RT-PCR assays for pestivirus detection target the highly conserved 5'-UTR. 5,7,[11][12][13]19 826299V DIXXX10.1177/1040638719826299Pestivirus detection with pan-bovine pestivirus primersMonteiro et al We employed a newly designed set of primers (BP189-389) to screen the sera of beef calves for pestiviruses in a gel-based RT-PCR. Serum samples positive for BVDV antigens in an antigen ELISA (n = 135) were submitted to RT-PCR using different sets of primers targeting the 5'-UTR of the viral genome (Table 1).…”

mentioning

confidence: 99%

Detection of bovine pestiviruses in sera of beef calves by a RT-PCR based on a newly designed set of pan–bovine pestivirus primers

et al. 2019

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The pestiviruses bovine viral diarrhea virus 1 and 2 (BVDV-1 and -2, respectively) and HoBi-like pestivirus (HoBiPeV) are important pathogens of cattle, and a number of reverse-transcription PCR (RT-PCR)-based assays have been developed for their detection in clinical specimens. We evaluated a newly designed set of pan-bovine pestivirus primers (BP189-389) in a gel-based RT-PCR screening test for pestiviruses in the sera of beef calves destined for export from southern Brazil. Serum samples positive for BVDV antigens by an antigen ELISA (n = 135) were submitted to RT-PCR assays using different sets of primers, followed by nucleotide sequencing of the amplicons. RT-PCR with pestivirus primers 324-326 detected 110 positive samples: BVDV-1 (n = 62), BVDV-2 (n = 38), and HoBiPeV (n = 10). A PCR using primers HCV90-368 detected 97 positive samples (64 BVDV-1; 33 BVDV-2). An additional RT-PCR round using BVDV-2-specific primers (2F-2R) detected 45 positive samples (including 38 detected by primers 324-326 and 33 by HCV90-368); whereas a RT-PCR using HoBiPeV-specific primers (N2-R5) detected 26 positive samples (including 10 detected by primers 324-326).The assay using the primers BP189-389 detected all 135 ELISA-positive samples, including the 26 HoBiPeV detected by primers N2-R5. Our results demonstrated that primers BP189-389 compare favorably against other primer sets in the detection of bovine pestiviruses, especially HoBiPeV. This conventional PCR may be useful for efficient detection of pestiviruses in bovine sera and other specimens as well, especially in laboratories without real-time PCR equipment.

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Detection and genetic identification of pestiviruses in Brazilian lots of fetal bovine serum collected from 2006 to 2014

Cited by 16 publications

References 22 publications

Genetic identification of pestiviruses from beef cattle in Southern Brazil

Genetic identification of pestiviruses from beef cattle in Southern Brazil

Phylogenetic and evolutionary analysis of HoBi‐like pestivirus: Insights into origin and dispersal

Detection of bovine pestiviruses in sera of beef calves by a RT-PCR based on a newly designed set of pan–bovine pestivirus primers

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