Detection and genetic polymorphism of human herpes virus type 8 in endemic or epidemic Kaposi's sarcoma from West and Central Africa, and South America

Fouchard, Nathalie; Lacoste, Vincent; Couppié, Pierre; Develoux, M.; Mauclère, Philippe; Hervé, Vincent; Pradinaud, R.; Bestetti, Giovanna; Huerre, Michel; Tekaia, Fredj; Thé, Guy de; Gessain, Antoine

doi:10.1002/(sici)1097-0215(20000115)85:2%3c166::aid-ijc3%3e3.0.co;2-l

Cited by 27 publications

(31 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[9,10,12,14,15] For example, several short targets of HHV-8 from AIDS-KS lesions were selected to identify distinctive molecular pattern, but with restricted coverage of the repertoire of HHV-8 genotypes. [17–19,12,20–23] Nowadays, sequence analysis of highly variable ORF K1 regions (i.e., VR1 and VR2) has allowed the identification of 7 major subtypes or genotypes of HHV-8 (A, B, C, D, E, F, and Z), all exhibiting clear ethnic and geographic clustering. [10,12,15,21,23–27] In addition, the ORF-K1 encodes for a transmembrane signaling molecule that plays an important role in the lifecycle of HHV-8, provoking cellular activation, endothelial cell immortalization and works synergistically with HIV-1 Tat to promote tumors.…”

Section: Introductionmentioning

confidence: 99%

Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma

Tozetto‐Mendoza¹,

Ibrahim²,

Tateno

et al. 2016

Medicine

View full text Add to dashboard Cite

AIDS-associated Kaposi's sarcoma (AIDS-KS) caused by human herpes virus 8 (HHV-8) is the most severe and resistant form of KS tumor. Our aim was to verify whether there is an association between HHV-8 variability and development of AIDS-KS in Brazil by comparing the HHV-8 variability between individuals without and with KS. Saliva samples and blood, when available, were analyzed by polymerase chain reaction (PCR) techniques for detection of the fragments of ORF K1 of HHV-8, which were then genotyped and analyzed regarding the genetic variability. Our study described 106 positive cases for HHV-8 in the saliva from 751 AIDS patients without previous KS. In addition, we performed a phylogenetic analysis of HHV-8 in 34 of the 106 AIDS patients without KS and in 33 of the 37 patients with active KS. The distribution of HHV-8 genotypes A, B, C, and F in AIDS individuals was indistinguishable by comparing non-KS and KS groups, as well as regarding ethnicity. Considering the KS group, genotype B was associated with better prognosis of KS tumor. Interestingly, we found a particular profile of diversity within clade C and 2 recombinant patterns of HHV-8 in the saliva of AIDS individuals without KS. We emphasize the need to achieve standard genotyping protocol for ORF K1 amplification, thus allowing for substantial detection of HHV-8 variants. Our findings can shed light on the role of HHV-8 variability in the pathogenesis of AIDS-KS.

show abstract

Section: Introductionmentioning

confidence: 99%

Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma

Tozetto‐Mendoza¹,

Ibrahim²,

Tateno

et al. 2016

Medicine

View full text Add to dashboard Cite

show abstract

“…For example, in a study of AIDS-KS, IFA was able to detect HHV-8 antibodies in 50% (latent) to 100% (lytic) of the patients, whereas, nested PCR detected infection in only 33% [57]. The data from a minority of reports showed that PCR was a more favorable assay in isolated cases [279] or that serology and PCR were comparable [280]. In a composite set of 642 samples from numerous reports, 69% were concordant in their PCR and serology results.…”

Section: Hhv-8 Diagnosticsmentioning

confidence: 99%

“…PCR appears to be very useful when detecting HHV-8 directly in the KS lesions, with sensitivity approaching 100% [50,51,183,279]. However, PBMCs from KS patients were observed to have fewer instances (~50%) of detectable viral sequences [50,51,55].…”

Section: Hhv-8 Diagnosticsmentioning

confidence: 99%

Human herpesvirus 8 – A novel human pathogen

Edelman

2005

Virol J

View full text Add to dashboard Cite

In 1994, Chang and Moore reported on the latest of the gammaherpesviruses to infect humans, human herpesvirus 8 (HHV-8) [1]. This novel herpesvirus has and continues to present challenges to define its scope of involvement in human disease. In this review, aspects of HHV-8 infection are discussed, such as, the human immune response, viral pathogenesis and transmission, viral disease entities, and the virus's epidemiology with an emphasis on HHV-8 diagnostics.

show abstract

“…Subgroup A1–4 and subtype C are predominant among populations of European descent [30], [32], [36], [42], [43] and in some regions of Asia [44], [45], [46]. Subgroup B1–4 and clade A5 are predominant in Sub-Saharan Africa [31], [34], [35], [47], [48], [49], [50]. The present work aimed at gaining new insights into the KSHV epidemiology and genetic diversity in Cameroon, in Western Central Africa.…”

Section: Introductionmentioning

confidence: 99%

Epidemiology and Genetic Variability of HHV-8/KSHV in Pygmy and Bantu Populations in Cameroon

et al. 2014

Self Cite

View full text Add to dashboard Cite

BackgroundKaposi's sarcoma associated herpesvirus (KSHV/HHV-8) is the causal agent of all forms of Kaposi sarcoma. Molecular epidemiology of the variable K1 region identified five major subtypes exhibiting a clear geographical clustering. The present study is designed to gain new insights into the KSHV epidemiology and genetic diversity in Cameroon.Methodology/Principal FindingsBantu and Pygmy populations from remote rural villages were studied. Antibodies directed against latent nuclear antigens (LANA) were detected by indirect immunofluorescence using BC3 cells. Peripheral blood cell DNAs were subjected to a nested PCR amplifying a 737 bp K1 gene fragment. Consensus sequences were phylogenetically analyzed. We studied 2,063 persons (967 females, 1,096 males, mean age 39 years), either Bantus (1,276) or Pygmies (787). The Bantu group was older (42 versus 35 years: P<10−4). KSHV anti-LANA seroprevalence was of 37.2% (768/2063), with a significant increase with age (P<10−4) but no difference according to sex. Seroprevalence, as well as the anti-LANA antibodies titres, were higher in Bantus (43.2%) than in Pygmies (27.6%) (P<10−4), independently of age. We generated 29 K1 sequences, comprising 24 Bantus and five Pygmies. These sequences belonged to A5 (24 cases) or B (five cases) subtypes. They exhibited neither geographical nor ethnic aggregation. A5 strains showed a wide genetic diversity while the B strains were more homogenous and belonged to the B1 subgroup.ConclusionThese data demonstrate high KSHV seroprevalence in the two major populations living in Southern and Eastern Cameroon with presence of mostly genetically diverse A5 but also B K1 subtypes.

show abstract

Detection and genetic polymorphism of human herpes virus type 8 in endemic or epidemic Kaposi's sarcoma from West and Central Africa, and South America

Cited by 27 publications

References 0 publications

Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma

Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma

Human herpesvirus 8 – A novel human pathogen

Epidemiology and Genetic Variability of HHV-8/KSHV in Pygmy and Bantu Populations in Cameroon

Contact Info

Product

Resources

About