2013
DOI: 10.1292/jvms.12-0567
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Detection and Genotype of <i>Encephalitozoon cuniculi</i> DNA from Urine and Feces of Pet Rabbits in Japan

Abstract: ABSTRACT. A newly developed nested polymerase chain reaction (PCR) assay targeting the internal transcribed spacer (ITS) region of the ribosomal DNA was applied to detect and characterize Encephalitozoon cuniculi DNA from pet rabbits in Japan. The analysis was carried out using 257 urinary samples and 314 fecal samples collected from 307 pet rabbits in the age group of 1 month to 12 years from 30 different prefectures of Japan and 107 fecal samples and 3 urinary samples collected from 1-month-old rabbits from … Show more

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Cited by 10 publications
(4 citation statements)
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“…The presence of the parasitic DNA in the urine of 26.21% of the studied pet rabbits, in comparision with results of study performed in Japan, where the DNA of parasites was detected in urine of 7.78% animals (Kimura et al 2013) indicates that E. cuniculi infections occur widely in pet rabbits in Poland and are a significant cause of neurological disorders in those animals.…”
Section: Resultssupporting
confidence: 85%
“…The presence of the parasitic DNA in the urine of 26.21% of the studied pet rabbits, in comparision with results of study performed in Japan, where the DNA of parasites was detected in urine of 7.78% animals (Kimura et al 2013) indicates that E. cuniculi infections occur widely in pet rabbits in Poland and are a significant cause of neurological disorders in those animals.…”
Section: Resultssupporting
confidence: 85%
“…The origin of E. cuniculi infection in rabbits in Tenerife is unknown. Despite the limited sample size, the prevalence of E. cuniculi obtained in fecal samples in this study (4%; 2/50) is similar to that found in fecal samples from pet rabbits in China (5.8%; 34/584) [ 17 ] and from farmed rabbits in Japan (5.6%; 6/107) [ 76 ] using PCR.…”
Section: Discussionsupporting
confidence: 77%
“…Considering the type of sample, the shedding of microsporidia spores in urine appears to be more common than in feces, as reported in the studies carried out by Valencakova et al [ 28 ] and Kimura et al [ 76 ]. The brain and kidney are the most frequently parasitized organ by E. cuniculi [ 66 , 67 ], followed by lung, heart, liver, and intestine.…”
Section: Discussionmentioning
confidence: 92%
“…Serology was chosen considered that in rabbits with chronic E. cuniculi infections excretion of the spores into urine and feces is short and intermittent [10,16], and direct methods could lead to underestimate the real prevalence. Could the authors provide any data about sensitivity and specificity of the commercial ELISA kit used?…”
Section: Discussionmentioning
confidence: 99%