Detection and Genotypic Characterisation of Rotavirus from Piglets and In-Contact Children of Assam, a North-Eastern State of India

Ahmed, S. P.; Bora, Durlav Prasad; Hazarika, R. A.; Tamuly, Shantanu; Deka, Naba Kumar; Gogoi, A.; Malik, Yashpal Singh

doi:10.5958/0973-9149.2017.00012.0

Cited by 2 publications

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“…The BRVA G10P[ 11 ] genotype is one of the predominant rotavirus genotypes circulating in bovines throughout India and the world [ 37 ]. The occurrence of the G10P[ 11 ] combination strain in 38.50% of samples is in harmony with other studies in India [ 28 38 ]. G10P[ 11 ] has been detected in clinical and subclinical cases in Indian children and is assumed to have been acquired through zoonotic transmission [ 39 ].…”

Section: Discussionsupporting

confidence: 90%

Determining genetic diversity of prevalent G and P genotype of Bovine Rotavirus A from neonatal calves of Gujarat, India

Golaviya,

Mathakiya,

Jakhesara

et al. 2024

J Vet Sci

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Importance Neonatal calf diarrhea is a major cause of mortality in newborn calves worldwide, posing a significant challenge in bovine herds. Group A Bovine Rotaviruses ( BRVA ) are the primary contributors to severe gastroenteritis in calves under two months old. Objectives This study examined the prevalence and molecular characterization of BRVA in neonatal calves in Gujarat, India. Methods Sixty-nine diarrheic fecal samples were collected and subjected to various molecular methods of BRVA detection, isolation, and characterization. Results The latex agglutination test (LAT), electropherotyping (RNA-PAGE), and reverse transcription polymerase chain reaction revealed positivity rates of 39.13%, 20.30%, and 37.70%, respectively. RNA-PAGE identified 11 bands with a 4:2:3:2 migration pattern, indicative of the segmented genome of BRVA . BRVA was successfully isolated from LAT-positive samples, with 26 samples exhibiting clear cytopathic effects upon passage in MA-104 cell lines. Genotyping identified G10 as the predominant G genotype, with P[11] genotypes comprising 76.92% of the isolates. The most common G/P combination was G10P[11], highlighting its zoonotic potential. Conclusions and Relevance These findings underscore the importance of molecular detection and genotyping for effective vaccine development. This study provides crucial insights into the prevalent G and P genotypes of BRVA in Gujarat, India, aiding in the development of targeted control measures.

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Section: Discussionsupporting

confidence: 90%

Determining genetic diversity of prevalent G and P genotype of Bovine Rotavirus A from neonatal calves of Gujarat, India

Golaviya,

Mathakiya,

Jakhesara

et al. 2024

J Vet Sci

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“…There is a wide distribution of rotavirus associated piglet diarrhea in different parts of India [ 2 , 3 , 21 , 22 ] with reports of higher occurrence in the north eastern states [ 12 , 14 , 22 , 23 ]. In the present study, out of 320 samples (120 from Assam and 200 from Tripura) screened by Monoclonal Antibody based sELISA (Premier RotacloneTM, Meridian Biosciences, United States) 130 samples (40.62%) were detected to be positive for RVA with an OD measure which ranged between 0.150 and 0.351.…”

Section: Resultsmentioning

confidence: 99%

“…A sequence based study from Assam reported the presence of major G and [P] combination to be G1P[ 7 ], G1P[ 13 ] and G1P[ 23 ] from porcine infected with RVA [ 25 ]. Another genotypic characterization study from Assam and Arunachal Pradesh of north east India revealed distribution of G3P[ 6 ], G3P[ 7 ], G9P[ 6 ], G9P[ 7 ], G10P[ 7 ], G10P[ 8 ] and G9P[ 8 ] [ 11 , 12 ]. However, from the present study, the distribution of G and P types came out to be G1, G3 and G8 from Assam, G4 type from Tripura and a single P[ 6 ] type from both the states.…”

Section: Resultsmentioning

confidence: 99%

“…From global epidemiological surveys, 12 different G-types (G1 to G6, G8 to G12 and G26) and 13 different P-types (P [1], P [5] to P [8], P [11], P [13], P [19], P [23], P [26], P[27], P[32], P[34]) have been identified and reported to be associated with piglet diarrhea caused by RVA [8][9][10]. In India, 6 types of G (G3, G4, G6, G9, G10, G12) and 5 types of P (P [6], P [7], P [8], P [13], P [19]) of RVA have been detected from pigs so far (11)(12)(13). Due to the segmented genome of RV, there is an increase diversification taking place in RV strains [13].…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of porcine group A rotavirus to contain piglet diarrhea for productivity enhancement in North East India

Das

Handique

2021

VirusDis.

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Gastrointestinal tract infections with acute diarrhea are indeed a serious cause of concern that held back the swine industry of North East India and among the various etiological agents causing the disease, group A rotavirus is the most common. In this regard, molecular characterization of the viral strains circulating in the region is of utmost importance for a strategic approach to control the disease. Thus, in the present study, diarrheic fecal samples from piglets of Tripura and Assam were evaluated for genotypic characterization of VP7 and VP4 gene of the virus. The samples positive for group A rotavirus were subjected to G and P type determination by nested- multiplex RT PCR as well as nucleotide sequencing of VP7 and VP4 gene. Nested- multiplex RT PCR revealed the presence of porcine group A rotavirus in the genotypic combination of G1P[6], G3P[6], G8P[6] and G4P[6] in the two states of North East India. Nucleotide sequencing of VP7 and VP4 genes however, revealed emergence of an unusual genotypic combination G26P[13]. VP7 and VP4 nucleotide and its deduced amino acid sequences when compared with global isolates, showed that the present strains shared closest similarity to the porcine rotavirus strain TJ4-1from Japan and hp140 from Eastern India. Identification of a novel genotype G26P[13] in the RVA infected pig population of Tripura and Assam, suggested presence of a greater diversity in rotavirus strains in the region. Thus, it evokes the need for continuous monitoring to assess and consider the strain variability in the design of a suitable vaccine candidate against the virus. Supplementary Information The online version of this article (10.1007/s13337-021-00659-6) contains supplementary material, which is available to authorized users.

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Detection and Genotypic Characterisation of Rotavirus from Piglets and In-Contact Children of Assam, a North-Eastern State of India

Cited by 2 publications

References 0 publications

Determining genetic diversity of prevalent G and P genotype of Bovine Rotavirus A from neonatal calves of Gujarat, India

Determining genetic diversity of prevalent G and P genotype of Bovine Rotavirus A from neonatal calves of Gujarat, India

Molecular characterization of porcine group A rotavirus to contain piglet diarrhea for productivity enhancement in North East India

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