Detection and Genotyping of Human Papillomavirus DNA Using Polymerase Chain Reaction Short PCR Fragment 10–Line Probe Assay in Abnormal Papanicolaou-Stained Cervicovaginal Smears

Méndez, Morelva Toro de; Bosch, Antonio Llombart

doi:10.1159/000325382

Cited by 4 publications

(3 citation statements)

References 41 publications

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“…The distribution of HPV genotypes in this study was consistent with that reported in studies previously [20,29,30,34]. HPV16 has been consistently identified as the most frequent HR-HPV.…”

Section: Discussionsupporting

confidence: 91%

“…Our results fall within this range. This prevalence of multiple HPV infection is higher than found in other studies conducted in Spain in which the values range between 4.7% and 52.1% [6,29,30,34,36,38,57]. Furthermore, in a study of patients with abnormal cytology obtained a higher value (58.1%) [35] than found in this study.…”

Section: Discussioncontrasting

confidence: 79%

“…Furthermore, we found highest prevalence of HPV (60.5%) in patients <40 years, even higher than that reported in other studies carried out in Spanish women, probably because it encompasses a different age group [6]. In previous studies conducted in this same context, HPV infection was positive in 90% of the patients with abnormal cytology and a mean age of 40 years [29], and in 43.3% in patients with mean age of 40.9 years of the routine screening [30].…”

Section: Discussioncontrasting

confidence: 64%

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Prevalence of Human Papillomavirus (HPV) Genotypes and Multiple Infections in Routine Cervical Cancer Screening in a Spanish Regional Population

Méndez¹

2013

SOJ Microbiol Infect Dis

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BackgroundCervical cancer is the second most common cancer in women worldwide and the most common cause of mortality in underdeveloped and developing countries [1,2]. Persistent infection with certain oncogenic high-risk (HR) types of Human Papillomavirus (HPV) is the required factor for the development of invasive cervical cancer and precursor lesions [3][4][5][6].Overall, the prevalence and distribution of HPV infection in cervical lesions has been determined in many geographical regions of the world, this can vary depending on several factors including epidemiological differences in the populations studied and the methodology used for molecular detection and typing of HPV DNA [7]. Over 120 types of HPV have been classified as either low (LR-HPV) or high risk (HR-HPV), according to their oncogenic potential [8,9]. Within the high-risk genotypes, HPV types 16 and HPV 18 are most often associated with cancer and squamous intraepithelial lesion [5,6,10,11,12]. Nevertheless, the risk of neoplasia for other types of HPV as well as for multiple HPV infection has not yet been established.Because current strategies for the prevention of cervical cancer and their precancerous lesions are based on the HPV genotyping [13] and prophylactic vaccines [14][15][16], it seems necessary to determine the types of HPV most commonly associated with malignant cell transformation in different geographical areas in order to establish effective preventive measures according to the epidemiology of the population. This study was to know the prevalence and distribution of cervical HPV infection in women attending routine cervical cancer screening in the Valencia region (Spain). Study designLiquid-based cytology (LBC) of cervical specimens was collected during routine screening visits, between June 20, 2011 and September 9, 2011 in the Clinical Hospital of Valencia, Spain. LBC samples were processed using the Thin Prep® 5000 processor (Madrid, Spain). For HPV detection and genotyping, we used the residual cervical sample material available from the same Thin Prep Pap test vial previously used for cytological screening. DNA was extracted from the residual materials of LBC by QIAamp® DNA mini kit (IZASA, Valencia, Spain), according to the manufacturer's instructions. HPV testing was performed by INNO-Lipa HPV genotyping Extra Reverse Hybridization Line Probe Assay kit (Innogenetics®, Barcelona, Spain), according to the manufacturer's instructions. Briefly, HPV detection and genotyping was based on PCR amplification of a 65pb fragment, within the L1 region of the HPV genome, using broad-spectrum SPF10 biotinylated primers. PCR was performed in a final AbstractBackground: Infection with human papillomavirus (HPV) has been identified as the primary cause of cervical cancer. Persistent infection with high risk-HPV is the required factor for the development of cervical cancer.

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“…The distribution of HPV genotypes in this study was consistent with that reported in studies previously [20,29,30,34]. HPV16 has been consistently identified as the most frequent HR-HPV.…”

Section: Discussionsupporting

confidence: 91%

Section: Discussioncontrasting

confidence: 79%

Section: Discussioncontrasting

confidence: 64%

See 1 more Smart Citation

Prevalence of Human Papillomavirus (HPV) Genotypes and Multiple Infections in Routine Cervical Cancer Screening in a Spanish Regional Population

Méndez¹

2013

SOJ Microbiol Infect Dis

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Molecular and Nanotechnologic Approaches to Etiologic Diagnosis of Infectious Syndromes

et al. 2011

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Infectious diseases are a major global public health problem. Multiple agents are now recognized to cause indistinguishable illnesses. The term 'syndrome' applies to such situations, for which early and rapid diagnosis of the infecting agent would enable prompt and appropriate therapy. Public health physicians would also get timely information on the specific etiology of the infectious syndrome, facilitating intervention at the community level in the face of outbreaks or epidemics. A variety of molecular techniques have been evaluated for rapid diagnosis of infectious syndromes. These techniques include real-time multiplex PCR, DNA microarray, loop-mediated isothermal amplification, and other similar assays. This review surveys such state-of-the-art technologies.

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Cervical Lymphadenopathy Associated with Hashimoto’s Thyroiditis

Paksoy¹,

Yazal²

2009

Acta Cytologica

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Detection and Genotyping of Human Papillomavirus DNA Using Polymerase Chain Reaction Short PCR Fragment 10–Line Probe Assay in Abnormal Papanicolaou-Stained Cervicovaginal Smears

Cited by 4 publications

References 41 publications

Prevalence of Human Papillomavirus (HPV) Genotypes and Multiple Infections in Routine Cervical Cancer Screening in a Spanish Regional Population

Prevalence of Human Papillomavirus (HPV) Genotypes and Multiple Infections in Routine Cervical Cancer Screening in a Spanish Regional Population

Molecular and Nanotechnologic Approaches to Etiologic Diagnosis of Infectious Syndromes

Cervical Lymphadenopathy Associated with Hashimoto’s Thyroiditis

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