Detection and genotyping of human papillomavirus in cervical samples from Italian patients

Francesco, Maria Antonia De; Gargiulo, Franco; Schreiber, C.; Ciravolo, Giuseppe; Salinaro, F.; Manca, N.

doi:10.1002/jmv.20306

Cited by 39 publications

(31 citation statements)

References 24 publications

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“…High prevalence in CSW in Spain had been previously described, both in Spanish (44) and in migrant CSW individuals (9). The rate of oncogenic HPV prevalence in the general population for Spanish women agrees with data previously published in Spain (12,20) and are similar to those of others conducted in European countries, such as Italy, with rates of prevalence of between 6.6 and 7.1% (10,33).…”

Section: Discussionsupporting

confidence: 90%

Oncogenic Human Papillomavirus (HPV) Type Distribution and HPV Type 16 E6 Variants in Two Spanish Population Groups with Different Levels of HPV Infection Risk

et al. 2006

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The aim of this study is to determine oncogenic human papillomavirus (HPV) types and HPV type 16 (HPV16) variant distribution in two Spanish population groups, commercial sex workers and imprisoned women (CSW/IPW) and the general population. A multicenter cross-sectional study of 1,889 women from five clinical settings in two Spanish cities was conducted from May to November 2004. Oncogenic HPV infection was tested by an Hybrid Capture II (HC2) test, and positive samples were genotyped by direct sequencing using three different primer sets in L1 (MY09/11 and GP5؉/GP6؉) and E6/E7. HPV16 variants were identified by sequencing the E6, E2, and L1 regions. Four hundred twenty-five samples were positive for the HC2 test, 31.5% from CSW/IPW and 10.7% from the general population. HPV16 was the most frequent type. Distinct profiles of oncogenic HPV type prevalence were observed across the two populations. In order of decreasing frequency, HPV types 16, 31, 58, 66, 56, and 18 were most frequent in CSW/IPW women, and types 16, 31, 52, 68, 51, and 53 were most frequent in the general population. We analyzed HPV16 intratype variants, and a large majority (78.7%) belonged to the European lineage. AA variants were detected in 16.0% of cases. African variants belonging to classes Af1 (4.0%) and Af2 (1.3%) were detected. Different HPV types and HPV16 intratype variants are involved in oncogenic HPV infections in our population. These results suggest that HPV type distribution differs in CSW/IPW women and in the general population, although further analysis is necessary.Different epidemiological studies reveal that human papillomavirus (HPV) prevalence depends largely on age and sexual practices and that it shows major differences across geographic areas. HPV prevalence has been described to be higher in Latin America and sub-Saharan Africa and lower in Asia and Europe (12,15,17,30,32,35,40,43). More than 100 types of HPV have been identified to date, of which at least 42 are associated with infections of the genital tract (42). Different genotype prevalence profiles have also been observed in different geographic areas (8). Vaccines directed against HPV type 16 (HPV16) and HPV18, present in approximately 70% of cervical cancers worldwide, are currently under evaluation and have shown impressive reductions of 90% in the incidence of HPV infection in the case of types 16 and 18 (16, 18, 22, 27, 45). The preliminary data suggest that bivalent HPV16 and HPV18 vaccine may also protect against HPV16-related types (31, 33, 35, 52, and 58) and HPV18-related types (39, 45, 59, 68, and 85) (G. Dubin, 22nd International Papillomavirus Conference and Clinical Workshop 2005, Oral Communication F-03, 2005). In order to design and evaluate the health impact of HPV infections and the potential benefits of the implementation of future vaccines, it is necessary to know the distribution of oncogenic HPV types in different geographic regions and in different populations (29).In Spain, which has one of the lowest rates of cervical cancer worldwid...

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Section: Discussionsupporting

confidence: 90%

Oncogenic Human Papillomavirus (HPV) Type Distribution and HPV Type 16 E6 Variants in Two Spanish Population Groups with Different Levels of HPV Infection Risk

et al. 2006

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“…GP5+/GP6+ primers amplify a broad spectrum of HPV genotypes by targeting a 150-bp fragment within the L1 open reading frame of the HPV genome (31). All samples and controls, independent of gel results, were subjected to the reverse line blot (RLB) for detection of 12 high-risk HPV types (16,18,31,33,35,39,45, 51, 52, 56, 58, and 59), 1 probably carcinogenic HPV type (68), and 5 possibly carcinogenic HPV types (26, 53, 66, 73, and 82; ref. 32).…”

Section: Laboratory Proceduresmentioning

confidence: 99%

“…INNO-LiPA genotyping kit was also used on the GP5+/GP6+ PCR-positive but RLB-negative samples because it allows for detection of 28 HPV types (16,18 ). This step was done only in Florence.…”

Section: Laboratory Proceduresmentioning

confidence: 99%

“…Five articles reported results for histologically confirmed invasive cancers (18)(19)(20)22, and 27), six reported results for preinvasive lesions according to histologic findings (17)(18)(19)(20)(21)(22)(23)(24), six reported results only according to cytologic results (25)(26)(27)(28)(29), and one by both classifications (17). Results are summarized in Table 1.…”

Section: Systematic Reviewmentioning

confidence: 99%

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Prevalence of Human Papillomavirus Types in High-Grade Cervical Intraepithelial Neoplasia and Cancer in Italy

Carozzi

Tornesello

Burroni

et al. 2010

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Background: The aim of this multicentric study was to identify human papillomavirus (HPV) type distribution in invasive cervical cancer and high-grade cervical intraepithelial neoplasia 2/3 (CIN2/3) in Italy.Methods: Cases were sampled through the electronic databases at the pathology units of eight centers in six regions from central and southern Italy. HPV types were detected from paraffin-embedded tissue samples and cervical specimens through amplification of HPV DNA with GP5+/GP6+ primers, followed by genotyping with reverse line blot (RLB). Untyped HPV-positive samples were sequenced. HPV-negative samples underwent nested PCR, followed by either RLB or sequencing. Finally, the remaining HPV-negative samples were amplified with primers targeting the virus E6 to E7 regions.Results: From 1,162 cases initially selected, 722 samples were further analyzed: 144 CIN2, 385 CIN3, 157 invasive squamous carcinomas, and 36 adenocarcinomas. Samples (6.9%) were HPV negative. The proportion of HPV16/18 was 60.8%, 76.6%, and 78.8% in CIN2, CIN3, and invasive cancers, respectively (P trend = 0.004). There was a significant decreasing trend of HPV16/18 with age in invasive cancers, going from 92% in women <35 years to 73% in women >55 years (P = 0.036). The proportion of coinfections was 16.8%, 15.5%, and 10.0% in CIN2, CIN3, and invasive cancers, respectively (P trend = 0.07).Conclusions: The proportion of invasive cancers caused by HPV16/18 decreases with age at diagnosis. Impact: The absolute risk of an invasive cancer due to non-HPV16/18 in women under 35 is extremely low. This finding might prompt us to rise the age at which public HPV screening for vaccinated women should start. Cancer Epidemiol Biomarkers Prev; 19(9); 2389-400. ©2010 AACR.

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“…Various evidences reveal that the human papillomavirus (HPV) is the single most important etiologic agent in CC. More than 100 different HPV genotypes have been identified, and of those, more than 40 genotypes are involved in infection of female genital tract (1) . HPV genotypes have been classified into high-risk (HR) and low-risk (LR) types according to molecular epidemiologic studies that provide risk estimates and functional evidence of the oncogenic potential of the various HPV genotypes (2) .…”

mentioning

confidence: 99%

Distribution of human papillomavirus genotypes in the patients with cervical carcinoma and its precursors in Zhejiang Province, China

Hong¹,

Ye²,

Chen³

et al. 2008

International Journal of Gynecological Cancer

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Various evidences reveal that the human papillomavirus (HPV) is the single most important etiologic agent in cervical carcinoma (CC). To investigate the distribution of HPV genotypes in the patients with CC and its precursors in Zhejiang Province, China, a total of 631 eligible samples from patients in Zhejiang Province with CC (N ¼ 181), cervical intraepithelial neoplasia (CIN) II-III (N ¼ 345), and CIN I (N ¼ 105) were detected. Age-matched samples of 217 women without cervical neoplasia were detected as control. An improved polymerase chain reaction (PCR)-restriction fragment length polymorphism assay validated by Hybrid Capture II and PCR sequencing was designed for HPV genotype. The prevalence of HPV was 95.0% in CC, 88.4% in CIN II-III, and 73.3% in CIN I, while only 41.9% in control. High-risk/low-risk HPV ratio showed a significant trend of increase with increased grade of CIN and transformation to carcinoma. A total of 24 HPV genotypes were detected in CC and its precursors. Of those, HPV 16 (65.2%), 18 (9.4%), and 58 (9.4%) were the major HPV genotypes in CC, while HPV 16

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Detection and genotyping of human papillomavirus in cervical samples from Italian patients

Cited by 39 publications

References 24 publications

Oncogenic Human Papillomavirus (HPV) Type Distribution and HPV Type 16 E6 Variants in Two Spanish Population Groups with Different Levels of HPV Infection Risk

Oncogenic Human Papillomavirus (HPV) Type Distribution and HPV Type 16 E6 Variants in Two Spanish Population Groups with Different Levels of HPV Infection Risk

Prevalence of Human Papillomavirus Types in High-Grade Cervical Intraepithelial Neoplasia and Cancer in Italy

Distribution of human papillomavirus genotypes in the patients with cervical carcinoma and its precursors in Zhejiang Province, China

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