Detection and Identification of a Novel Potexvirus Infecting Allium by Paramagnetic Beads Ssrna Isolation and One Tube Rt-PCR Assay With a New Potexvirus Genus Primer Set

Plantago asiatica mosaic virus detected in Lilium in the UK

“…Total RNA was extracted from the sample using magnetic beads and tested with a Verso 1‐step RT‐ PCR Reddymix kit (Thermo Scientific, USA) using generic potexvirus primers, reverse Potex 4 and forward Potex 5 primers (Miglino et al , 2006). A 280 bp amplicon was purified and sent for sequencing (Genbank Accession No.…”

Detection of Plantago asiatica mosaic virus in lily hybrid plants (Lilium spp.) in Costa Rica grown from imported bulbs

“…Transmission electron microscopy (TEM) image from sample T188 leaf tissue with several flexuous rods in the cytoplasm of a cell (c); scale bar represent 500 nm. Symptoms observed on Nicotiana benthamiana after mechanical inoculation with sample T189, 22 days after inoculation (dai) (d), with sample T188, 36 dai (e), and buffer-inoculated plant, 22 dai (f) (Miglino et al 2006), and ii. the species Plantago asiatica mosaic virus, CP-F2/CP-R3 (Hammond et al 2015) d Asymptomatic plants e Cymbidium mosaic virus (CymMV) infecting Phaius tankervilliae (Moreira et al 1998) f No final contig.…”

“…the species Plantago asiatica mosaic virus, CP-F2/CP-R3 (Hammond et al 2015) d Asymptomatic plants e Cymbidium mosaic virus (CymMV) infecting Phaius tankervilliae (Moreira et al 1998) f No final contig. Sequencing reaction with forward primer resulted in PlAMV sequence; however, the reaction with reverse primer failed PlAMV using 'tospovirus-specific' primers was verified by testing with primer pairs for general potexvirus detection (Potex 4 / Potex 5, Miglino et al 2006) and for specific detection of PlAMV (PlAMV CP-F2 / CP-R3, Hammond et al 2015). As negative controls, four symptomless lily plants (two pots, 2 plants per pot) were purchased at a supermarket in San José Province.…”

“…The partial sequences obtained with primer pairs Potex 4 / Potex 5 (Miglino et al 2006) and CP-F2 / CP-R3 (Hammond et al 2015) for samples T188, T189 and L3 were aligned with corresponding sequences from available PlAMV genomes from GenBank (Table 2) using MUSCLE (Edgar 2004) algorithm in MEGA7 (Kumar et al 2016). The species Tulip virus X (Yamaji et al 2001) and Cymbidium mosaic virus (Wong et al 1997) were included as outgroups.…”