Detection and identification of azithromycin resistance mutations on &lt;em&gt;Treponema pallidum&lt;/em&gt; 23S rRNA gene by nested multiplex polymerase chain reaction

Gultom, Desy A.; Rosana, Yeva; Efendi, Ida; Indriatmi, Wresti; Yasmon, Andi

doi:10.13181/mji.v26i2.1543

Cited by 4 publications

(6 citation statements)

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“…The use of the Treponema pallidum target gene and the differing clinical specimens affected the results of the multiplex nested PCR. (13,14,21) The target gene and PCR method used in this study refers to the target gene and PCR method used by Gultom et al (16) The study by Gayet-Ageron et al (20) used the 47kDa Treponema pallidum target gene with the real-time PCR method. The study by Grange et al (10) used the tpp47 Treponema pallidum target gene with the nested PCR method.…”

Section: Discussionmentioning

confidence: 99%

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Multiplex nested polymerase chain reaction for Treponema pallidum using blood is more sensitive than using serum

et al. 2018

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UNIVERSA MEDICINAMultiplex nested polymerase chain reaction for Treponema pallidum using blood is more sensitive than using serum Ida Effendi* , **, Yeva Rosana**, Andi Yasmon**, and Wresti Indriatmi*** BACKGROUND Syphilis is a multistage disease transmitted primarily through sexual intercourse. Nowadays, the polymerase chain reaction (PCR) test for Treponema pallidum has been widely used and is expected to overcome problems in diagnostic tests for syphilis. The Treponema pallidum PCR is influenced by type of specimens, PCR methods and target genes. This study aimed to assess the use of blood and serum in multiplex nested PCR for Treponema pallidum, targeting the 23S rRNA. METHODSA cross-sectional study was conducted from April 2015 -April 2016. Sampling was carried out consecutively among patients with clinical features of secondary syphilis who came to Sexually Transmitted Disease (STD) clinics in Jakarta. All sera were also tested with Rapid Plasma Reagin (RPR) and Treponema pallidum Hemagglutination Assay (TPHA) assay, which was considered as the gold standard for this study. We determined the sensitivity and specificity of the multiplex nested PCR for Treponema pallidum using blood and serum. RESULTS PCR test was performed on 122 clinical specimens (61 blood and 61 serum).The positive results of PCR test on blood was 22.95% and serum was 6.56%, while the positive results of serology was 68.85%. The sensitivity of Treponema pallidum multiplex nested PCR on blood was 30.95% compared to serum 9.52% (p=0.006). PCR test on blood is able to detect 3.25 times higher than serum. CONCLUSIONThe use of blood has a higher proportion of positives compared to serum in Treponema pallidum multiplex nested PCR using 23S rRNA target gene.

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Section: Discussionmentioning

confidence: 99%

“…(16) Therefore this study uses multiplex nested PCR to determine the better specimen between blood and serum for the application of this test.…”

Section: Introductionmentioning

confidence: 99%

Multiplex nested polymerase chain reaction for Treponema pallidum using blood is more sensitive than using serum

et al. 2018

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“…Eight factors were associated with syphilis based on the Poisson regression models: (1) reduced schooling (illiterate or elementary school education), (2) a monthly income of less than the minimum wage in the past 12 mo, (3) the use of illicit drugs (inhaled/injectable) at some time, (4) having unprotected sex in the previous 7 d, (5) having unprotected sex with a regular client in the previous 7 d, (6) having unprotected sex with a client who paid more for the encounter in the previous 7 d, (7) >7 y as a sex worker and ( 8) not having a medical/gynecological examination in the previous 12 mo (Table 4). Spending >7 y as a sex worker was identified as the principal risk factor for syphilis in the FSWs (RP>17.0).…”

Section: Factors Associated With Syphilismentioning

confidence: 99%

“…However, due to the global scarcity of this drug and cases of allergy to penicillin in many patients, alternative types of medication, such as macrolide antibiotics (which include azithromycin and erythromycin), have increasingly been used in recent years. 5 Although macrolides have been shown to be effective for the treatment of syphilis, an increasing incidence of drug-resistant strains has been reported in recent years. Drug resistance is associated with the point mutations A2058G and A2059G in the 23S rRNA gene, which have been detected in some strains of T. pallidum.…”

Section: Introductionmentioning

confidence: 99%

Treponema pallidum in female sex workers from the Brazilian Marajó Archipelago: prevalence, risk factors, drug-resistant mutations and coinfections

Coelho¹,

Souza

Costa

et al. 2020

Transactions of the Royal Society of Tropical Medicine and Hygiene

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Background Female sex workers (FSWs) are an especially vulnerable group for syphilis and other sexually transmitted infection (STIs). This study determined the prevalence of syphilis in FSWs and factors associated with this disease in the Marajó Archipelago (northern Brazil), as well as the frequency of point mutations (A2058G and A2059G) in the 23S rRNA gene of Treponema pallidum and coinfections with hepatitis B virus (HBV), hepatitis C virus (HCV) and hepatitis D virus (HDV). Methods FSWs were diagnosed using a rapid qualitative test and the isolates were evaluated for the presence of point mutations by real-time PCR. Blood samples with T. pallidum were tested for the presence of HBV, HCV and HDV by ELISA and confirmed by real-time PCR. The factors associated with syphilis were identified using Poisson regression models. Results Overall, 41.1% FSWs tested positive for syphilis and 23.5% were infected with strains having A2058G/A2059G point mutations. HBV (23.0%) and HCV (8.1%) were detected among FSWs with syphilis. Six factors were associated with syphilis: low levels of education, reduced income, drug use, unprotected sex, a lengthy career in prostitution and a lack of regular medical check-ups. Conclusions These findings indicate an urgent need for implementation of effective strategies to diagnose, prevent and treat syphilis, as well as other STIs, in this Brazilian region.

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“…Gultom dkk. 30 mengembangkan multiplex nested PCR untuk deteksi sekaligus identifikasi Treponema pallidum resisten azitromisin dengan gen target 23S rRNA.…”

Section: Multiplex Nested Pcrunclassified

Pemeriksaan Molekular Treponema pallidum

Effendi

2019

J. Kdokt Meditek

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Treponema pallidum adalah bakteri penyebab sifilis. Sifilis ditemukan pada abad ke XV di dataran Eropa, menyebar ke seluruh dunia dan menjadi isu global sampai saat ini. Sifilis dikenal dengan sebutan raja singa merupakan salah satu penyakit infeksi menular seksual yang penularannya tidak hanya melalui hubungan seksual. Infeksi lokal yang disebabkan oleh masuknya Treponema pallidum akan berkembang cepat menjadi sistemik dan bahkan dapat mengancam nyawa. Treponema pallidum tidak dapat dikultur secara in vitro. Pemeriksaan penunjang untuk menegakkan diagnosis mengandalkan uji serologi. Saat ini, pemeriksaan standar untuk sifilis menggunakan mikroskop lapangan gelap sulit dilakukan. Pemeriksaan baru untuk mendeteksi Treponema pallidum menggunakan uji molekuler sudah mulai dikembangkan sejak abad XX. Penulisan ini mengambil ulasan dari beberapa jurnal penelitian yang menggunakan PCR sebagai alat diagnostik Treponema pallidum untuk membandingkan metode-metode pemeriksaan molekuler PCR Treponema pallidum. Spesifisitas PCR Treponema pallidum mencapai 100%. Sensitivisitas PCR bervariasi untuk setiap gen target dan jenis spesimen yang digunakan. Metode multiplex PCR banyak digunakan karena dapat mendeteksi Treponema pallidum dan patogen lain secara bersamaan. Pemeriksaan molekuler PCR Treponema pallidum masih perlu dikembangkan lebih lanjut di Indonesia. Kata kunci : Treponema pallidum, sifilis, uji serologi, mikroskop, pemeriksaan molekular

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Detection and identification of azithromycin resistance mutations on <em>Treponema pallidum</em> 23S rRNA gene by nested multiplex polymerase chain reaction

Cited by 4 publications

References 27 publications

Multiplex nested polymerase chain reaction for Treponema pallidum using blood is more sensitive than using serum

Multiplex nested polymerase chain reaction for Treponema pallidum using blood is more sensitive than using serum

Treponema pallidum in female sex workers from the Brazilian Marajó Archipelago: prevalence, risk factors, drug-resistant mutations and coinfections

Pemeriksaan Molekular Treponema pallidum

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