Detection and identification of equine Theileria and Babesia species by reverse line blotting: epidemiological survey and phylogenetic analysis

Nagore, Daniel; Garcı́a-Sanmartı́n, Josune; Garcı́a-Pérez, Ana L.; Juste, Ramón A.; Hurtado, Ana

doi:10.1016/j.vetpar.2004.04.010

Cited by 122 publications

(119 citation statements)

References 24 publications

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“…equi sequence from South Africa (accession number: Z15105) (Allsopp et al, 1994 Nagore et al (2004a) …”

Section: Resultsmentioning

confidence: 99%

“…Subsequently, other studies led to the identification of two genetically distinct Theileria and two other Babesia genotypes infecting Spanish horses (Criado-Fornelio et al, 2004 andNagore et al, 2004a). Another study (Criado et al, 2006) showed genetically distinct T. equi-like isolates.…”

Section: Discussionmentioning

confidence: 99%

“…Advances in molecular biological techniques have resulted in the improved detection, identification and genetic characterization of many haemoparasites (Caccio et al, 2000 andNagore et al, 2004a). PCR has been applied for the detection of many species of Babesia and Theileria and has been shown to have higher sensitivity and specificity compared with serological assays (Geysen et al, 2003, Buling et al, 2007, Jefferies et al, 2007and Sibeko et al, 2008.…”

mentioning

confidence: 99%

“…A sensitive and specific reverse line blot (RLB) hybridization assay, based on a sequence specific PCR that targets parasite 18S rRNA genes, has been developed for detection of Theileria and Babesia infections, including piroplasmosis in horses (Gubbels et al, 1999 andNagore et al, 2004a). This assay enables the identification of mixed infections and has also proven to be a valuable tool in the identification of novel piroplasm species or genotypes (Georges et al, 2001, Criado-Fornelio et al, 2004, Nagore et al, 2004a, Nagore et al, 2004band Nijhof et al, 2005.…”

mentioning

confidence: 99%

“…This assay enables the identification of mixed infections and has also proven to be a valuable tool in the identification of novel piroplasm species or genotypes (Georges et al, 2001, Criado-Fornelio et al, 2004, Nagore et al, 2004a, Nagore et al, 2004band Nijhof et al, 2005.…”

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confidence: 99%

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Sequence heterogeneity in the 18S rRNA gene within Theileria equi and Babesia caballi from horses in South Africa

Bhoora

Franssen

Oosthuizen

et al. 2009

Veterinary Parasitology

131

126

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“…equi sequence from South Africa (accession number: Z15105) (Allsopp et al, 1994 Nagore et al (2004a) …”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Sequence heterogeneity in the 18S rRNA gene within Theileria equi and Babesia caballi from horses in South Africa

Bhoora

Franssen

Oosthuizen

et al. 2009

Veterinary Parasitology

131

126

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Molecular detection of piroplasms in domestic donkeys in Xinjiang, China

Cui,

Cao,

et al. 2024

Veterinary Medicine & Sci

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BackgroundPiroplasmosis is a common and prevalent tick‐borne disease that affects equids.ObjectivesTo determine the infection and molecular characteristics of the piroplasms in donkeys from Xinjiang, northwestern China, we undertook a cross sectional study by collecting representative samples across several counties within the region.MethodsA total of 344 blood samples were collected from adult domestic donkeys from 13 counties in Xinjiang. PCR was conducted to test for T. equi and B. caballi in the blood samples based on the equine merozoite antigen‐1 (Ema‐1) gene and the 48 kDa rhoptry protein (BC48) gene, respectively.ResultsSixteen blood samples tested positive for piroplasms and the overall infection rate was 4.7% (16/344). Seven of the 13 counties were positive for piroplasms. Among the 16 piroplasm‐positive samples, 15 were singly infected with T. equi with an infection rate of 4.4% (15/344), and coinfection with T. equi and B. caballi was detected in one sample (0.3%, 1/344) from Wushi. Four T. equi sequence genotypes were identified and grouped into different branches of the evolutionary trees.ConclusionThese findings suggest that the infection rate of piroplasms is low in domestic donkeys in southern Xinjiang and that T. equi genotypes have a regional distribution.

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Reverse Line Blot Hybridization with Species-Specific Oligonucleotide Probes: Application to Piroplasm Detection

Hurtado

2014

Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies

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Reverse line blot (RLB) hybridization has become a well-established and widely used method for the multiplex identification of several Babesia and Theileria species in hosts and tick vectors. The procedure is based on the simultaneous PCR amplification of a polymorphic region of the 18S rRNA gene from different piroplasms followed by identification of the individual species by hybridization to species-specific oligonucleotide probes covalently linked to a nylon membrane in a macroarray format.

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Detection and identification of equine Theileria and Babesia species by reverse line blotting: epidemiological survey and phylogenetic analysis

Cited by 122 publications

References 24 publications

Sequence heterogeneity in the 18S rRNA gene within Theileria equi and Babesia caballi from horses in South Africa

Sequence heterogeneity in the 18S rRNA gene within Theileria equi and Babesia caballi from horses in South Africa

Molecular detection of piroplasms in domestic donkeys in Xinjiang, China

Reverse Line Blot Hybridization with Species-Specific Oligonucleotide Probes: Application to Piroplasm Detection

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