Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee Apis dorsata

Tajabadi, Naser; Mardan, Makhdzir; Manap, Mohd Yazid Abdul; Mustafa, Shuhaimi; Meimandipour, Amir; Nateghi, Leila

doi:10.1007/s13592-011-0069-x

Cited by 40 publications

(38 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…trophallaxis). Bacteria from the Lactobacillus (LAB) genus are some of the most important intestinal symbionts, and the LAB members most frequently isolated from honeybees are: Lactobacillus kunkeei, L. plantarum, L. pentosus, L. fermentum, L. floricola, L. acidophilus and L. johnsonii (4,12,27,41,42,44). Honeybee Lactobacillus bacteria, as all members of this genus, produce a short chain fatty acids which can be absorbed through the rectal wall and provide additional nutrition.…”

Section: Praca Oryginalnamentioning

confidence: 99%

Impact of vertebrate probiotics on honeybee yeast microbiota and on the course of nosemosis

Ptaszyńska¹,

Borsuk²,

Mułenko³

et al. 2016

Medycyna Weterynaryjna

View full text Add to dashboard Cite

The natural intestine microbiota of honeybees is dominated by Gram-negative bacteria from such families as: Enterobacteriaceae, Alcaligenaceae and Pseudomonadaceae, less numerous are Gram-positive bacteria, yeasts and other fungi. Our research was focused on the impact of commercial vertebrae probiotics, on honeybees’ intestine yeast number, hemolymph lysozyme activity and nosemosis development. The diet of honeybees was supplemented with two vertebrate probiotics recommended in beekeeping management. The former consisted of Lactobacillus casei, L. plantarum, Saccharomyces cerevisiae, and Rhodopseudomonas palustris. The latter consisted of L. acidophilus, L. delbrueckii, and Bifidobacterium bifidum. Yeasts isolated from honeybees’ intestine and detected in our research belonged to two genera: Candida and Saccharomyces. The average number of yeast Colony Forming Units (CFUs) in uninfected honeybees fed only sugar syrup was 3850 (±400.33) and after Nosema spp. infection the number rose to 19500 (±1644.40) per one honeybee. The addition of a probiotic decreased the number of detected yeast CFUs in both groups of infected and uninfected honeybees. Nosema infection did not affect lysozyme activity; therefore it was the administration of probiotics that had the main impact on the rise of lysozyme level. Most likely very vivid probiotic bacteria were treated as invaders by honeybees’ immune defense, which resulted in almost 40% rise observed in lysozyme activity. Furthermore, probiotic supplementation accelerated the nosemosis development even twofold. Probably, increased acidity of the honeybees’ intestine, which is a consequence of the uncontrolled growth of lactic acid bacteria, created conditions favorable to faster nosemosis development. Therefore, every administration of diet supplements with live microorganisms whose impact on honeybees biology and physiology is not well studied should be carried out with the greatest caution.

show abstract

Section: Praca Oryginalnamentioning

confidence: 99%

Impact of vertebrate probiotics on honeybee yeast microbiota and on the course of nosemosis

Ptaszyńska¹,

Borsuk²,

Mułenko³

et al. 2016

Medycyna Weterynaryjna

View full text Add to dashboard Cite

show abstract

“…Lactic acid bacteria are remarkably abundant in honey stomach of honey bees and mainly belong to the genera Lactobacillus , Bifidobacterium , and Enterococcus (Tajabadi et al , 2011a, 2011b). …”

Section: Discussionmentioning

confidence: 99%

“…The isolates were incubated for 3–4 days at 37 °C under anaerobic conditions, using Anaerobic Jars with Anaerocult (Merck, Darmstadt, Germany) (Tajabadi et al , 2011b). Next, 92 colonies with varying morphologies were chosen and sub-cultured as pure cultures.…”

Section: Methodsmentioning

confidence: 99%

“…They are found in a wide variety of habitats, including vegetable and the gastrointestinal tracts of animals, like honeybees (Tannock, 2004; Schrezenmeir and de Vrese, 2001; Fujisawa and Mitsuoka, 1996) and are employed in manufacturing of fermented foods, such as soybean products, fish products, vegetable products, bread, alcoholic beverages, dairy, and for cheese production (Kandler et al , 1986; Rhee et al , 2011; Caplice and Fitzgerald, 1999). The dominant LAB species in the honey stomach of honeybees’ A. mellifera , A. dorsata , and bumblebees have been reported to belong to the Lactobacillus genus (Olofsson and Vasquez, 2008; Vásquez et al , 2009; Tajabadi et al , 2011b). …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from honey stomach of honeybee

Tajabadi¹,

Mardan²,

Saari³

et al. 2013

Braz. J. Microbiol.

Self Cite

View full text Add to dashboard Cite

This study aimed to isolate and identify Lactobacillus in the honey stomach of honeybee Apis dorsata. Samples of honeybee were collected from A. dorsata colonies in different bee trees and Lactobacillus bacteria isolated from honey stomachs. Ninety two isolates were Gram-stained and tested for catalase reaction. By using bacterial universal primers, the 16S rDNA gene from DNA of bacterial colonies amplified with polymerase chain reaction (PCR). Forty-nine bacterial 16S rDNA gene were sequenced and entrusted in GenBank. Phylogenetic analysis showed they were different phylotypes of Lactobacillus. Two of them were most closely relevant to the previously described species Lactobacillus plantarum. Other two phylotypes were identified to be closely related to Lactobacillus pentosus. However, only one phylotype was found to be distantly linked to the Lactobacillus fermentum. The outcomes of the present study indicated that L. plantarum, L. pentosus, and L. fermentum were the dominant lactobacilli in the honey stomach of honeybee A. dorsata collected during the dry season from Malaysia forest area - specifically “Melaleuca in Terengganu”.

show abstract

“…Within the most isolated species of Lactobacilli genus are those belonging to the species [17], and Lactobacillus acidophilus. Among Biidobacterium genus, novel species were identiied, B. asteroids and B. coryneform [8].…”

Section: Honey As Probiotic Sourcementioning

confidence: 99%

Honey as a Functional Food

Luchese¹,

Prudêncio²,

Guerra³

2017

Honey Analysis

View full text Add to dashboard Cite

The most well-known functional properties of honey are its antioxidant and antimicrobial activities. The bioactive components of honey are afected by the lora from which it is produced and by geographical variations. Phenolic compounds promote, among other activities, high antioxidant action, being capable of minimizing intracellular oxidative damage associated with cellular aging, apoptosis and neurodegenerative diseases. A living cell system would provide a beter platform for determining antioxidant activity, since the bioactive honey compounds can act modulating antioxidant defense gene expression. Indeed, phenolic compounds, amino acids and reducing sugars are among the substances responsible for honey antioxidant activity. Most of phenolic compounds also exert antimicrobial activity against a number of pathogens and spoilage microorganisms. The antimicrobial activity of honey is also due to the action of enzymes. In addition, honey was found to contain lactic acid bacteria (LAB), which itself produce a myriad of active compounds that remain in variable amounts in mature honey. In addition, these antioxidant compounds might play a key role as prebiotic, protecting and stimulating growth of probiotic bacteria. Oligosaccharides present in honey are well-known prebiotic substances stimulating growth, activity and protecting probiotic bacteria during passage through the gastrointestinal tract and during storage of the products. This chapter describes the main bioactive components of honey, especially with respect to the phenolic compounds and their antioxidant activity and assay methods.

show abstract

Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee Apis dorsata

Cited by 40 publications

References 26 publications

Impact of vertebrate probiotics on honeybee yeast microbiota and on the course of nosemosis

Impact of vertebrate probiotics on honeybee yeast microbiota and on the course of nosemosis

Identification of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from honey stomach of honeybee

Honey as a Functional Food

Contact Info

Product

Resources

About