Detection and Identification of Phytoplasmas in Pomegranate Trees with Yellows Symptoms

Gazel, Mona; Çağlayan, Kadriye; Başpinar, Hüseyin; Mejía, Juan Fernando; Paltrinieri, Samanta; Bertaccini, Assunta; Contaldo, Nicoletta

doi:10.1111/jph.12401

Cited by 20 publications

(12 citation statements)

References 10 publications

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“…The systemic phytopathogen is recognized by a large number of diseases by affecting many plants including vineyard, fruit trees, ornamental plants, and vegetables, as well as insects (Çağlar and Elbeaoino, 2013;Ember et al, 2011;Pracros et al, 2006;Gazel et al, 2016, Weisburg et al, 1989Oshima et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Güller¹,

Usta²

2020

Türk Tarım Ve Doğa Bilimleri Dergisi

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Phytoplasma-type symptoms were noted in tomato (Lycopersicum esculentum L.) in Bingöl province of Turkey. The remarkable symptoms include witch's broom, rosetting, purple curled and crispy leaves, small leaves in the upper branches and excessively elongated calyx. Genomic DNA isolation was performed to identify possible pathogens from the leaves of 11 plants with and without symptom. In the Nested-PCR test performed using universal primer sets, DNA bands of approximately 1200 bp size were obtained in 4 of 11 samples. Randomly chosen two DNA bands primed R16F2n/R16R2 were cloned into an appropriate plasmid vector to further characterizations. The recombinant plasmid DNAs purified were sequenced in both directions. Molecular assays of the 16S rRNA sequence confirmed the existence of the "Canditatus Phytoplasma solani" (16SrXII-A group) (similarity coefficient 1.00) (Accession no: MT279680) and the "Canditatus Phytoplasma trifolii" (16SrVI-A group) (similarity coefficient 1.00) (Accession no: MT279852) in the infected tomato samples. The isolates associated with tomato-phytoplasma were named as 'Bingöl D11' and 'Bingöl D90' isolates, respectively. The phylogenetic dendrogram created also confirmed where both pathogens belong. This current paper is documented in the first record of "Ca. P. solani" (16SrXII-A) and "Ca. P. trifolii" (16SrVI-A) in naturally diseased tomato in Bingöl of Turkey.

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Section: Introductionmentioning

confidence: 99%

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Güller¹,

Usta²

2020

Türk Tarım Ve Doğa Bilimleri Dergisi

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“…On the other hand aster yellows phytoplasmas ('Ca. P. asteris') were reported in Turkey in cherry (Çağlayan et al 2013) and in pomegranate (Gazel et al 2016) and in peach in Canada (Zunnoon-Khan et al 2010) indicating their spreading to fruit tree host plants.…”

Section: Discussionmentioning

confidence: 98%

Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey

Orel

Paltrinieri²,

Ertunç

et al. 2019

Bitki Koruma Bülteni

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Apple, pear and apricot trees showing phytoplasma associated symptoms from Ankara and Isparta provinces were sampled and investigated to verify the presence of phytoplasma-associated diseases. Totally 31 samples were tested with phytoplasma universal and group specific primers and the PCR products were restricted using Tru1I, RsaI and SspI endonucleases. Different RFLP profiles were obtained and selected samples were directly sequenced. The apple samples were found infected with 16SrX-A ('Candidatus Phytoplasma mali'), while the majority of the pear samples were infected with 16SrX-A and 16SrX-C subgroup phytoplasmas in mixed infection. The 16SrX-B ('Candidatus Phytoplasma prunorum'), 16SrX-C ('Candidatus Phytoplasma pyri') and mixed infection of 16SrX-A/16SrX-C and 16SrX-C/16SrI (aster yellows) were detected in the apricot samples. In this study the 16SrI phytoplasmas in apricot and the mixed phytoplasma infections in pear and apricot trees were detected in Turkey for the first time.

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“…Phytoplasma associated symptoms have been observed in many cultivated fruit, vegetable, ornamental and field crops in Turkey (Alp et al 2016;Başpınar et al 1993;. Çağlar et al 2010;Çağlar and Elbeaoino 2013;Özdemir et al 2009;Özdemir and Saygılı 2012;Sertkaya et al 2004;Sertkaya et al 2005;Sertkaya et al 2007;Sertkaya et al 2008;Ulubaş Serçe et al 2006;Gazel et al 2016). Field cucumber (Cucumis sativus L.) has been considered one of the most economically important vegetable crops in Turkey.…”

Section: Indroductionmentioning

confidence: 99%

Hıyar (Cucumis sativus L.) Bitkisinde Aynı Simptomatolojiye Sahip İki Farklı Fitoplazma etmeninin 16S rDNA'sının RFLP ve Nükleotid Dizi Analizleri ile Teşhisi ve Karakterizasyonu

Usta

Güller

Sipahioğlu

2017

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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Phytoplasma-like symptoms were observed in cucumbers (Cucumis sativus L.) in Van province of Turkey. The major symptoms observed were severe dwarfing, witches' broom, rosetting, little leaf, and sterility of plants. Genomic DNA of 8 symptomatic and non-symptomatic plant leaves was isolated for the detection of pathogenic DNA. Of the 8 cucumber leaf samples tested by nested polymerase chain reaction (Nested-PCR), the four yielded the expected 1.25-kb DNA fragments when using universal primer pairs R16mF2/R16mR1 and R16F2n/R16R2. Randomly selected two DNA bands were further cloned into a proper plasmid vector. The recombinant plasmid DNA was sequenced bidirectionally. BLAST and virtual restriction fragment length polymorphism (RFLP) analyses of the 16S rDNA sequence revealed the presence of the "Candidatus Phytoplasma solani" (similarity coefficient 1.00) (GenBank accession no: KX977570) in one of the severely symptomatic cucumber samples and the "Candidatus Phytoplasma trifolii" (similarity coefficient 0.98) (GenBank accession no.: KR080212) in the other. The isolates were designated as Van-trifolii and Van-solani isolates, respectively. No significant differences were observed between the two different phytoplasmas' symptomatology. To the authors' knowledge, this is the first report of 'Ca. P. trifolii' and 'Ca. P. solani' in cucumbers in Turkey.

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Detection and Identification of Phytoplasmas in Pomegranate Trees with Yellows Symptoms

Cited by 20 publications

References 10 publications

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey

Hıyar (Cucumis sativus L.) Bitkisinde Aynı Simptomatolojiye Sahip İki Farklı Fitoplazma etmeninin 16S rDNA'sının RFLP ve Nükleotid Dizi Analizleri ile Teşhisi ve Karakterizasyonu

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