Detection and identification of platelet antibodies using a sensitive multiplex assay system–platelet antibody bead array

Abstract

The PABA is a rapid, highly sensitive and specific, multiplex bead-based assay for detecting human platelet antibodies. Such a simple yet high-throughput platform will facilitate practical, routine testing for the identification of platelet-specific antibodies.

Timely diagnosis and treatment of neonatal alloimmune thrombocytopenia caused by anti HPA-3a antibody

“…[16–20] Thus, HPA-3a would appear to be significantly immunogenic factor only less than HPA-1a and HPA-5b. With the development of technology, numerous new approaches provide highly sensitive and specific, multiplex bead-based assay for detecting human platelet antibodies, [21–23] which can contribute to a high diagnostic rate of the NAIT.…”

Patients treated with oxaliplatin are at risk for thrombocytopenia caused by multiple drug-dependent antibodies

“…The strongest antibodies were specific for dexamethasone and leucovorin in patient 1 and irinotecan in patient 2. In a platelet antibody bead array assay, 11 it was found that dexamethasone-and leucovorin-dependent antibodies from patient 1 and the irinotecan-dependent antibody from patient 2 recognized a IIb /b 3 integrin (GPIIb/IIIa) and the oxaliplatindependent antibody from patient 1 recognized GPIb/IX (supplemental Figure 1, available on the Blood Web site). Other DDAb targets were not identified.…”

Severe thrombocytopenia in a patient following liver transplantation caused by HPA‐1a antibodies produced by the liver donor

“…Detection and identification of platelet‐reactive antibodies was performed at the Platelet & Neutrophil Immunology International Reference Lab, BloodCenter of Wisconsin (BCW), Milwaukee using both a flow cytometry test with intact target platelets, and a highly sensitive and specific Platelet Antibody Bead Array (PABA) for detection and identification of platelet specific antibodies against human platelet alloantigens (HPA). Flow cytometry testing was performed as previously described .…”

“…Beads were washed 3 times and incubated with fluorescent anti‐human IgG (Jackson ImmunoResearch Labs, West Grove, PA) for detection of patient HPA antibodies. Bead fluorescence intensity was measured on a Luminex 200 (Luminex Corporation) and results determined using an in‐house developed software program to call HPA antibody specificity …”