Detection and Identification of Potyviruses in Zantedeschia

Pham, K.T.K.; Langeveld, S.A.; Lemmers, M.E.C.; Derks, A.F.L.M.

doi:10.17660/actahortic.2002.568.20

Cited by 15 publications

(9 citation statements)

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“…BLAST analysis of the CP nucleotide sequence revealed highest identity to konjac mosaic virus (KoMV) isolates (synonym zantedeschia mosaic virus) available in GenBank (EU544542, LC114491, LC114492, AB219545) with 96% to 99%. In Europe, KoMV was previously detected in Germany (Lesemann and Winter 2002) and in the Netherlands (Pham et al 2002). To our knowledge, this is the first report on the occurrence of KoMV in Hungary.…”

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confidence: 57%

First report of konjac mosaic virus in Zantedeschia from Hungary

2019

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In the spring of 2018 a distorted Zantedeschia 'Picasso' plant was observed in Nyársapát, Hungary. The plant was shorter, had narrower, smaller and more acuminate light green leaves with greenish-yellow longitudinal mosaic compared to healthy plants. The spathe did not open; outer color was light green with longitudinal purple stripes. Healthy plants had light purple outer spathe which opened normally. The arrival of warm weather and decreasing rate of plant growth caused masked symptoms, but the leaf margin remained chlorotic. These symptoms indicated/denoted a possible virus infection. Nicotiana tabacum cv. Xanthi, Nicotiana benthamiana and Chenopodium murale plants were inoculated, during a five week period no symptoms were observed. ACP-ELISA based on MAb PTY1 antibody (Jordan and Hammond 1991)provided by Agdiawere carried out in duplicate of the leaf sap of Zantedeschia plant. Absorbance values were 1.694 and 1.698, while negative controls were 0.002 and 0.007, respectively. These results indicated potyvirus infection. To identify the virus, RT-PCR amplification was carried out on the same leaf from which ELISA tests were performed. Universal potyvirus primers, poty7941 and poly T 2 were used, as earlier described (Salamon and Palkovics 2005). A single PCR product was cloned into pGEM®-T Easy vector and the nucleotide sequence was determined (GenBank accession No. MH979038), which was 1618 nt long, including the complete coat protein region. The sequence determined included a single XbaI restriction site; XbaI digestion of the original PCR product yielded only the two predicted Open Access This article is distributed under the terms of the Creative Comm ons Attribution 4.0 International License (http:// creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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confidence: 57%

First report of konjac mosaic virus in Zantedeschia from Hungary

2019

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“…was achieved with a broad spectrum monoclonal antibody. DSMZ AS 0537 in Germany (Lesemann and Winter, 2000) and in the Netherlands with monoclonal antibody PTY I (Pham et al. 2000).…”

Section: Reports Of "New" Viruses and New Diseases---1985-2003mentioning

confidence: 99%

Quarantine Viruses, Viroids and Phytoplasmas That Affect Movement of Ornamental Plants

Lawson¹,

Hsu²

2006

Acta Hortic.

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www.invasivespeeies.org.Newlnitiatives.html) provides a guide for trading partners and alerts them to pests of quarantine concern in the U.S. APHIS activities include an inspection at ports-of-entry, surveys of exotic pests, methods of risk mitigation and eradication programs. APHIS also oversees a post-entry program for vegetatively propagated materials entering the U.S. and a pie-clearance program from specific countries that allows products to enter without additional inspection following importation. The Pest List currently includes seventy viruses but only six of them infect ornamentals. Included are Arabis mosaic virus, Elm mottle virus, Raspberry ringspot virus, Tobacco ring.spot virus (Andean potato calico strain). Tomato b/ackring virus, and Colombian datura virus. During the last two years Raspberry ringspot virus, Tomato blackring virus, and Arabis mosaic virus were detected in samples sent to Agdia, Inc., (http://www.agdia.com). There are 93 genera and families of plants in the prohibited quarantine category in the U.S. regulations. Most are propagated vegetatively. There are very few restrictions on import of seeds. Prohibitions may apply to certain countries or to all countries depending on the risk assessment (United States Department of Agriculture (USDA) Title 7-Code of Federal Regulations-Part 319). Only four genera of cut flowers, including Rhododendron and azaleas, Camellia, Gardenia, Rosa and Svringu vu/guns are required to have written permits issued by the Animal and Plant Health Inspection Service of the USDA for entry into the U.S. Importers must also obtain phytosanitary certificates from the exporting country. The prohibited category includes many genera of nursery stock, plants, roots, bulbs and other plant products. Prohibited does not mean that all the materials classified as prohibited are restricted from entering the country (USDA Title 7-Code of Federal Regulations-Part 3 19). Prohibition is a function of both host and country of origin. Some articles that are intended for propagation may be in a restricted category that allows entry when specific requirements are fullfilled. For example. Chrysanthemum vegetative material can enter the country with a phytosanitary certificate from the nation of origin and a declaration that the greenhouse where the plants were grown was free of white rust based on visual inspection monthly for a period of four months prior to shipment. Imported Dianthus from Great Britian must be accompanied by a phytosanitary certificate and an additional declaration verifying that the propagated material was grown in a greenhouse free of Carnation etched ring virus, Carnation "streak' virus and Carnation "fleck" virus. In addition to the requirements imposed on the exporter, the importer may have to grow designated restricted articles in post-entry quarantine. Imported Chrysanthemum. Crocosmia, Dianthus, Gladiolus, Hibiscus, Hydrangea, Jasminum, Rosa, Syringa and Watsonia must all be grown in a protected environment that meets State regulations and must be perio...

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“…A newly identified calla virus, ZaMMV, was found widely spread in the fields in Taiwan and probably in other countries ). The major symptoms of calla lily induced by these four viruses are mosaic, yellow stripe, green island and mild mosaic (Zettler and Hartman 1987;Chang et al 2001;Pham et al 2002;Chen et al 2003;. CLLV alone does not produce symptoms in calla lilies and may not have a direct impact on the crop (Chen et al 2004).…”

Section: Introductionmentioning

confidence: 98%

Detection of four calla potyviruses by multiplex RT-PCR using nad5 mRNA as an internal control

Huang

Lee

et al. 2009

Eur J Plant Pathol

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Dasheen mosaic virus (DsMV), Turnip mosaic virus (TuMV), Konjac mosaic virus (KoMV) and Zantedeschia mild mosaic virus (ZaMMV) are important potyviruses previously identified in calla lily plants in Taiwan. In order to save time and cost of virus detection, a multiplex RT-PCR assay was developed for these calla potyviruses. Specific primers for each virus were designed based on the sequences of 3′ terminal region of respective viruses. To prevent false negative results, a primer pair specific to plant mitochondrial nad5 mRNA was used to produce a 185-bp fragment as an internal control of RT-PCR. The specificities of primers were confirmed by means of simplex and multiplex PCR assays. Optimal primer concentration ratio was identified by multiplex PCR assay. Total RNAs purified from virus-infected plants were used directly or mixed in different combinations, and then tested by multiplex RT-PCR. The result indicated that the expected RT-PCR products could be specifically amplified and identified on the basis of their molecular sizes. The detection sensitivity of multiplex RT-PCR was 25-625 times higher than that of indirect-ELISA (I-ELISA) depending on the virus. When applied to field surveys, multiplex RT-PCR could detect more single as well as mixed infection samples than I-ELISA. Accordingly, our multiplex RT-PCR assay provides a simple, rapid and reliable method for multiple potyvirus detection in calla lily.

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Detection and Identification of Potyviruses in Zantedeschia

Cited by 15 publications

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First report of konjac mosaic virus in Zantedeschia from Hungary

First report of konjac mosaic virus in Zantedeschia from Hungary

Quarantine Viruses, Viroids and Phytoplasmas That Affect Movement of Ornamental Plants

Detection of four calla potyviruses by multiplex RT-PCR using nad5 mRNA as an internal control

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