Detection and identification of TMV infecting tomato under protected cultivation in Paraná State

Plant virus-based nanoparticles (VNPs) are a novel class of nanocarriers with unique potential for biomedical applications. VNPs have many advantageous properties such as ease of manufacture and high degree of quality control. Their biocompatibility and biodegradability make them an attractive alternative to synthetic nanoparticles (NPs). Nevertheless, as with synthetic NPs, to be successful in drug delivery or imaging, the carriers need to overcome several biological barriers including innate immune recognition. Plasma opsonization can tag (V)NPs for clearance by the mononuclear phagocyte system (MPS), resulting in shortened circulation half lives and non-specific sequestration in non-targeted organs. PEG coatings have been traditionally used to ‘shield’ nanocarriers from immune surveillance. However, due to broad use of PEG in cosmetics and other industries, the prevalence of anti-PEG antibodies has been reported, which may limit the utility of PEGylation in nanomedicine. Alternative strategies are needed to tailor the in vivo properties of (plant virus-based) nanocarriers. We demonstrate the use of serum albumin (SA) as a viable alternative. We demonstrate that SA conjugation to tobacco mosaic virus (TMV)-based nanocarriers results in a ‘camouflage’ effect more effective than PEG coatings. SA-’camouflaged’ TMV particles exhibit decreased antibody recognition, as well as enhanced pharmacokinetics in a Balb/C mouse model. Therefore, SA-coatings may provide an alternative and improved coating technique to yield (plant virus-based) NPs with improved in vivo properties enhancing drug delivery and molecular imaging.

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“…vegetables) [42,43] and tobacco [44]. Antibodies against TMV, therefore, may be prevalent in the human population.…”

Section: Resultsmentioning

confidence: 99%

Serum albumin ‘camouflage’ of plant virus based nanoparticles prevents their antibody recognition and enhances pharmacokinetics

et al. 2016

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“…Viral vectors are primarily eliminated from the body through antibody clearance, and TMV-specific antibodies have been previously found in blood collected from both smokers and non-smokers, presumably due to the prevalence of the virus in crops and tobacco cigarettes. 35–38 Repeat administration of TMV-based therapeutics would also lead to development of TMV-specific antibodies, so it is critical to test the ability of stealth-coated particles to prevent immune recognition. Immuno dot blots with anti-TMV antibodies and fluorescent TMV particles were performed to test the shielding properties of the SA-TMV particles (Fig.…”

Section: Resultsmentioning

confidence: 99%

Cryo-electron tomography investigation of serum albumin-camouflaged tobacco mosaic virus nanoparticles

et al. 2017

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Nanoparticles offer great potential in drug delivery and imaging, but shielding strategies are necessary to increase circulation time and performance. Structure-function studies are required to define the design rules to achieve effective shielding. With several formulations reaching clinical testing and approval, the ability to assess and detail nanoparticle formulations at the single particle level is becoming increasingly important. To address this need, we use cryo-electron tomography (cryo-ET) to investigate stealth-coated nanoparticles. As a model system, we studied the soft matter nanotubes formed by tobacco mosaic virus (TMV) coated with human serum albumin (SA) stealth proteins. Cryo-ET and subtomogram averaging allow for visualization of individual SA molecules and determination of their orientations relative to the TMV surface, and also for measurement of the surface coverage provided by added stealth proteins. This information fills a critical gap in the understanding of the structural morphology of stealth-coated nanoparticles, and therefore cryo-ET may play an important role in guiding the development of future nanoparticle-based therapeutics.

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“…The detection of Artichoke latent virus (ArLV), TMV, TSWV, BBWV and RpRSV also conducted with RT-PCR and total RNAs were extracted according to Foissac et al (2005) The assays were performed with highly specific primers to Artichoke latent virus ArLV5BL: 5'-GAT CTA GCG ATA CAC ATG CAC AAC C-3' and ArLV3BL: 5'-CGC TCA AGC TCT CGA ACT AAC TGA AC-3' (Lumia et al, 2003). RT-PCR assays for TMV, TSWV, BBWV and RpRSV were performed with using highly specific primers designed by Silva et al (2008), Lotos et al (2009), Ferriol et al (2011) and Treiber (2010), respectively.…”

Section: Methodsmentioning

confidence: 99%

The New Report of Artichoke latent virus (ArLV) From Globe Artichoke in Turkey

Erkan¹,

Gümüş²,

Duman³

et al. 2014

Ege Üniversitesi Ziraat Fakültesi Dergisi

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n recent years, some of artichoke growers in Aegean region of Turkey stated that globe artichoke (Cynara cardunculus L. subsp. scolymus (L.) Hayek) plants in their fields have showed virus-like symptoms. So, in order to identify viruses infecting globe artichoke, a survey was conducted between April 2011 and March 2012 and totally 50 globe artichoke samples were collected from the production areas in the mentioned region from which was provided more than 30 % of total globe artichoke production in Turkey. The samples were analyzed by DAS-ELISA, RT-PCR and biological indexing methods for Artichoke latent virus (ArLV), Tobacco mosaic virus (TMV), Tomato spotted wilt virus (TSWV), Broad bean wilt virus (BBWV) and Raspberry ringspot virus (RpRSV). TMV, TSWV, BBWV and RpRSV were not detected in all of samples tested. In RT-PCR, amplicons of the expected size (ca. 282 bp) were obtained from 9 out of 35 symptomatic plant samples bearing virus-like symptoms. The amplified and sequenced 9 PCR products (Accession No: KC622053) showed 86 % nucleotide sequence identity with "Artichoke latent virus mRNA unknown function (GenBank Accession No:X87255.1)" isolate in BlastN analysis. Moreover, when mechanically inoculated with the sap from plant samples, that were positive for ArLV in RT-PCR, selected test plants produced the typical symptoms of ArLV infection. As to the result of the literature review on the topic in question, this is the latest report of natural ArLV infection from globe artichoke in Turkey.

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Detection and identification of TMV infecting tomato under protected cultivation in Paraná State

Abstract: During an inspection in plastic houses in

Cited by 12 publications

References 10 publications

Serum albumin ‘camouflage’ of plant virus based nanoparticles prevents their antibody recognition and enhances pharmacokinetics

Serum albumin ‘camouflage’ of plant virus based nanoparticles prevents their antibody recognition and enhances pharmacokinetics

Cryo-electron tomography investigation of serum albumin-camouflaged tobacco mosaic virus nanoparticles

The New Report of Artichoke latent virus (ArLV) From Globe Artichoke in Turkey

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