Detection and Imaging of the Plant Pathogen Response by Near‐Infrared Fluorescent Polyphenol Sensors

Nißler, Robert; Müller, Andrea T.; Dohrman, Frederike; Kurth, Larissa; Li, Han; Cosio, Eric G.; Flavel, Benjamin S.; Giraldo, Juan Pablo; Mithöfer, Axel; Kruss, Sebastian

doi:10.1002/anie.202108373

Cited by 40 publications

(25 citation statements)

References 76 publications

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“…Such nanomaterials contained some minor fractions of other chiralities after purification (Figure a); however, they also enabled one to obtain a ratiometric sensing of H 2 O 2 in leaf tissue (Figure b). More recently, PEG–phospholipid-modified SWCNTs were shown to be sensitive to plant secondary metabolites (polyphenols) . Soybean seedling (glycine max (G. max)) grew inside the sensor-agar, while challenging the plant with a pathogen-derived elicitor triggered a plant-defense response (polyphenol secretion), continuously monitored by NIR stand-off imaging, such sensors were able to depict pathogen-induced plant defense (Figure c).…”

Section: Monochiral Nanosensors For Precision Agriculturementioning

confidence: 99%

Prospects of Fluorescent Single-Chirality Carbon Nanotube-Based Biosensors

Nißler

Ackermann

et al. 2022

Anal. Chem.

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Semiconducting single-wall carbon nanotubes (SWCNTs) fluoresce in the near-infrared (NIR), and the emission wavelength depends on their structure (chirality). Interactions with other molecules affect their fluorescence, which has successfully been used for SWCNT-based molecular sensors. So far, most such sensors are assembled from crude mixtures of different SWCNT chiralities, which causes polydisperse sensor responses as well as spectral congestion and limits their performance. The advent of chirality-pure SWCNTs is about to overcome this limitation and paves the way for the next generation of biosensors. Here, we discuss the first examples of chirality-pure SWCNT-based fluorescent biosensors. We introduce routes to such sensors via aqueous two-phase extraction-assisted purification of SWCNTs and highlight the critical interplay between purification and surface modification procedures. Applications include the NIR detection and imaging of neurotransmitters, reactive oxygen species, lipids, bacterial motives, and plant metabolites. Most importantly, we outline a path toward how such monodisperse (chirality-pure) sensors will enable advanced multiplexed sensing with enhanced bioanalytical performance.

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Section: Monochiral Nanosensors For Precision Agriculturementioning

confidence: 99%

Prospects of Fluorescent Single-Chirality Carbon Nanotube-Based Biosensors

Nißler

Ackermann

et al. 2022

Anal. Chem.

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“…A key to all these applications is versatile non-covalent and recently covalent surface chemistry 53,94,95 . It enabled imaging of neurotransmitters 96-100 , reactive oxygen species [101][102][103][104][105][106][107] , polyphenols 108 , disease markers 33,53,109,110 and many other important analytes and biological targets. Translated to EB-NS, the versatile surface chemistry presented by us in this work will enable similar applications.…”

Section: Discussionmentioning

confidence: 99%

Covalently Functionalized Egyptian Blue Nanosheets for Near-Infrared Bioimaging

Selvaggio

Herrmann

Hill

et al. 2022

Preprint

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Fluorophores emitting in the near-infrared (NIR) wavelength region present optimal characteristics for photonics and especially bioimaging. Unfortunately, only few NIR fluorescent materials are known and even fewer are biocompatible. For this reason, the scientific interest in designing novel NIR fluorophores is very high. Egyptian Blue (CaCuSi4O10, EB) is a NIR fluorescent layered silicate that can be exfoliated into fluorescent nanosheets (EB-NS). So far, its surface chemistry has not been tailored but this is crucial for colloidal stability and biological targeting. Here, we demonstrate covalent surface functionalization of EB nanosheets (EBfunc) via Si-H activation using hydrosilanes with variable functionalities. EB-NS were first grafted with the visible fluorescent pyrene (Pyr) moieties to prove conjugation by colocalization of the Vis/NIR fluorescence on the (single) EB-NS level. The same procedure was performed and validated with carboxyl group (COOH)-containing hydrosilanes. These groups can serve as generic handle for further (bio)functionalization of the EB-NS surface. Finally, folic acid (FA) was conjugated to these COOH-functionalized EB-NS to target folic acid receptor-expressing cancer cells. These results highlight the potential of this surface chemistry approach to modify EB-NS and enable targeted NIR imaging for biomedical applications.

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“…In this expedition, fluorescent SWCNT represent again an archetypical platform owing to their high chemical sensitivity , coupled with NIR imaging capabilities that can be engineered for analyte sensing. , Several groups disseminated on the globe are working in this direction with demonstrations to sense, for example, proteins, neurotransmitters, arsenic, and plant pathogens …”

Section: Some Next Challenges: Placing the Complex Sample At The Core...mentioning

confidence: 99%

On Some Current Challenges in High-Resolution Optical Bioimaging

Bon

Cognet

2022

ACS Photonics

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In this Perspective we propose our current point of view and a suggestive roadmap on the field of high-resolution optical microscopy dedicated to bioimaging. Motivated by biological applications, researchers have indeed devised an impressive amount of strategies to address the diverse constraints of imaging and studying biological matter down to the molecular scale, making this interdisciplinary research field a vibrant forum for creativity. Throughout the discussion, we highlight several striking recent successes in this quest. We also identify some next challenges still ahead to apprehend biological questions in increasingly complex living organisms for integrative studies in a minimally invasive manner.

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Detection and Imaging of the Plant Pathogen Response by Near‐Infrared Fluorescent Polyphenol Sensors

Cited by 40 publications

References 76 publications

Prospects of Fluorescent Single-Chirality Carbon Nanotube-Based Biosensors

Prospects of Fluorescent Single-Chirality Carbon Nanotube-Based Biosensors

Covalently Functionalized Egyptian Blue Nanosheets for Near-Infrared Bioimaging

On Some Current Challenges in High-Resolution Optical Bioimaging

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