DETECTION AND INCIDENCE OF Geotrichum candidum IN FROZEN BLANCHED VEGETABLES

Splittstoesser, D. F.; Bowers, Jayson; Kerschner, L.; Wilkison, Martha

doi:10.1111/j.1365-2621.1980.tb04089.x

Cited by 15 publications

(4 citation statements)

References 2 publications

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“…It proliferates in refrigerated cakes (Mislivec et al . 1974) and in frozen branched vegetables (Splittstoesser et al. 1980).…”

Section: Introductionmentioning

confidence: 99%

“…However, together with some yeasts, G. candidum is the main agent responsible for the degradation of fresh cheeses and fruit and vegetable juices (Skoropad 1968;Cichowitz and Bandler 1980;Engel et al 1980). It proliferates in refrigerated cakes (Mislivec et al 1974) and in frozen branched vegetables (Splittstoesser et al 1980). It attacks fresh fruit from the orchard as well as warehoused fruit, in which it produces aqueous, viscous or slimy rot (Laville 1974).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity among Geotrichum candidum strains from various substrates studied using RAM and RAPD-PCR

et al. 2002

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Aims: Assessment of genetic diversity within the species Geotrichum candidum and development of tools to trace the strains that play an important role in the agro food industry. Methods and Results: RAM‐PCR and RAPD‐PCR techniques were assessed for their ability to discriminate 57 strains of various morphotypes, substrates and geographical origin. The techniques were complementary and, when combined, allowed us to discriminate isolates. Moreover, we established a link between a taxon and its occupation of an ecological niche, which should not be confused with the substrate of isolation. Conclusions: We observed a high degree of diversity, which could be linked to the variety of the ecological niches chosen and to the high degree of morphological polymorphism encountered within the species. Significance and Impact of the Study: Used in combination, RAM‐PCR and RAPD‐PCR permit traceability and monitoring systems for G. candidum strains during food processing.

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“…It proliferates in refrigerated cakes (Mislivec et al . 1974) and in frozen branched vegetables (Splittstoesser et al. 1980).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic diversity among Geotrichum candidum strains from various substrates studied using RAM and RAPD-PCR

et al. 2002

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“…These results and the synergistic combinations between hydrogen peroxide and some acids that we reported against bacteria in a previous work (Martin and Maris ) prompted us to test the disinfectant efficacy of hydrogen peroxide with different acids (four mineral and 13 organic acids) towards a panel of fungal strains including reference strains selected in the French standards as model for yeasts and fungi representative of contaminants found in the food industry (Splittstoesser et al . ; Anon ,b; Moreau ; Hamdy et al . ).…”

Section: Introductionmentioning

confidence: 97%

Synergism between hydrogen peroxide and seventeen acids against five agri-food-borne fungi and one yeast strain

Martin

Maris

2012

J Appl Microbiol

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Aims: The objective of this study was to evaluate fungicidal efficacy of hydrogen peroxide administered in combination with 17 mineral and organic acids authorized for use in the food industry. Methods and Results: The assays were performed on a 96-well microplate using a microdilution technique based on the checkerboard titration method. The six selected strains (one yeast and five fungi) were reference strains and strains representative of contaminating fungi found in the food industry. Each synergistic hydrogen peroxide/acid combination found after fifteen minutes contact time at 20°C in distilled water was then tested in conditions simulating four different use conditions. Twelve combinations were synergistic in distilled water, eleven of these remained synergistic with one or more of the four mineral and organic interfering substances selected. Hydrogen peroxide/formic acid combination remained effective against four strains and was never antagonistic against the other two fungi. Combinations with propionic acid and acetic acid stayed synergistic against two strains. Those with oxalic acid and lactic acid kept their synergism only against Candida albicans. No synergism was detected against Penicillium cyclopium. Conclusions: Synergistic combinations of disinfectants were revealed, among them the promising hydrogen peroxide/formic acid combination. Significance and Impact of the Study: A rapid screening method developed in our laboratory for bacteria was adapted to fungi and used to reveal the synergistic potential of disinfectants and/or sanitizers combinations.

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“…Growth has been observed in refrigerated cakes [42] and in frozen leaf vegetables [55] and attack on unripe fruits in orchards and ripe fruits in storage, causes a watery, thick or sticky rot [36]. G. candidum is also a commensal organism of human and animal digestive systems [27].…”

Section: Introductionmentioning

confidence: 99%

Identification of Geotrichum candidum at the species and strain level: proposal for a standardized protocol

Gente

Sohier

Coton³

et al. 2006

J Ind Microbiol Biotechnol

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In this study, the M13 primer was used to distinguish Geotrichum candidum from the anamorphic and teleomorphic forms of other arthrospore-forming species (discriminatory power = 0.99). For intraspecific characterization, the GATA4 primer showed the highest level of discrimination for G. candidum among the 20 microsatellite primers tested. A molecular typing protocol (DNA concentration, hybridization temperature and type of PCR machine) was optimized through a series of intra- and interlaboratory trials. This protocol was validated using 75 strains of G. candidum, one strain of G. capitatum and one strain of G. fragrans, and exhibited a discrimination score of 0.87. This method could therefore be used in the agro-food industries to identify and to evaluate biodiversity and trace strains of G. candidum. The results show that the GATA4 primer might be used to differentiate strains according to their ecological niche.

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DETECTION AND INCIDENCE OF Geotrichum candidum IN FROZEN BLANCHED VEGETABLES

Cited by 15 publications

References 2 publications

Genetic diversity among Geotrichum candidum strains from various substrates studied using RAM and RAPD-PCR

Genetic diversity among Geotrichum candidum strains from various substrates studied using RAM and RAPD-PCR

Synergism between hydrogen peroxide and seventeen acids against five agri-food-borne fungi and one yeast strain

Identification of Geotrichum candidum at the species and strain level: proposal for a standardized protocol

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