Detection and isolation of Duck Plague virus from field outbreaks in Assam, India

Neher, Samsun; Barman, Nagendra Nath; Bora, Durlav Prasad; Deka, Dipak; Tamuly, Shantanu; Deka, P. C.; Bharali, Arpita; Das, S. K.

doi:10.18805/ijar.b-3588

Cited by 3 publications

(4 citation statements)

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“…The DNA polymerase gene encodes UL30 protein, which is highly conserved in DPV ( Wallace et al ., 2000 ; OIE, 2012 ; Wu et al ., 2012 ; Ahamed et al ., 2015 ). UL30 is involved in DNA replication and nucleotide metabolism in DPV ( Li et al ., 2006 ; Neher et al ., 2019 ). It is widely used to construct phylogenetic trees and to indicate a distinct division among Alphaherpesvirinae, Betaherpesvirinae, and Gammaherpesvirinae ( Thureen and Keeler, 2006 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of duck plague virus from selected Haor areas of Bangladesh

Khan¹,

Islam²,

Sabuj³

et al. 2021

Open Vet J.

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Background: Duck viral enteritis, commonly known as duck plague (DP), is an acute and contagious fatal disease in ducks, geese, and swans caused by the DP virus (DPV). It poses a serious threat to the growth of duck farming in the Haor (wetland) areas of Bangladesh. Aim: This study aimed to detect the circulating DPV by molecular characterization, followed by phylogenetic analysis, targeting the UL30 gene in infected ducks from five Haor districts in Bangladesh and to observe the variation in the genome sequence between the field virus and vaccine strain of DPV. Methods: A total of 150 samples (liver, 50; intestine, 50; and oropharyngeal tissue, 50) were collected from DP-suspected sick/dead ducks from 50 affected farms in Kishoreganj, Netrokona, B. Baria, Habiganj, and Sunamganj districts in Bangladesh. For the identification of DPV in collected samples, polymerase chain reaction (PCR) was utilized. Nucleotide sequences of the amplified UL30 gene were compared with those of other DPV strains available in GenBank. Results: Of the 150 samples, 90 (60%) were found to be positive for DPV, as confirmed by PCR. Organ-wise prevalence was higher in the liver (72%), followed by the intestine (64%) and oropharyngeal tissue (44%). Regarding areas, the highest and lowest prevalence in the liver and intestine was observed in Habiganj and B. Baria, respectively, whereas the highest and lowest prevalence in the oropharyngeal tissue was observed in B. Baria and Habiganj, respectively. Two isolates, BAU/KA/DPV(B1)/2014 from Kishoreganj and BAU/KA/DPV(B4)/2014 from Sunamganj were sequenced, and phylogenetic analysis revealed that these isolates are evolutionarily closely related to Chinese isolates of DPV. Additionally, the isolates of DPV BAU/KA/DPV(B1)/2014 and BAU/KA/DPV(B4)/2014 showed the highest (98%) similarity to each other. The nucleotide sequence of the isolate BAU/KA/DPV(B1)/2014 exhibited higher nucleotide variability (246 nucleotides) than that of the vaccine strain (accession no. EU082088), which may affect protein function and additional drug sensitivity. Conclusion: Based on the findings of the molecular study, it can be assumed that the Bangladeshi isolates and all Chinese isolates of DPV may have a common ancestry.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of duck plague virus from selected Haor areas of Bangladesh

Khan¹,

Islam²,

Sabuj³

et al. 2021

Open Vet J.

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“…It targeted Ducks showing typical clinical signs of DP including the in contact Healthy ducks and the dead birds suspected to be dead of DPV infection to collect different clinical and postmortem samples for the study. Post Mortem (PM) examination was conducted according to (Neher et al, 2019, El-Tholoth et al, 2019 for dead and live infected birds and it scrutinized all internal organs for presence of typical DP lesions. During the PM review, visceral organs like the heart, esophagus, proventriculus, spleen, liver and intestine were collected.…”

Section: Methods Of Sample Collectionmentioning

confidence: 99%

“…In 1923 the first known occurrence of DVE was recorded in the Netherlands (Mondal et al, 2010, Baudet, 1923.The first DP Virus (DPV) isolation and identification study was published in Bangladesh in 1982, subsequently evaluating the immunogenicity of the DPV vaccine from local isolates (Wu et al, 2012a, Ahamed et al, 2015a, Hossain et al, 2005a, Islam et al, 2005. The morbidity and mortality rates of suspected birds vary between 5% and 100%, depending on the virulence of bird infection and immunological status (Neher et al, 2019) that causes acute hemorrhagic, septic, infectious, and fatal diseases among waterfowls such as ducks, geese, and swans, (Li et al, 2009, You et al, 2018, El-Tholoth et al, 2019, Wu et al, 2012a, which caused major economic losses in the commercial waterfowl industry around the world as a result of sudden deaths, condemnations, and reduced production of eggs (Cai et al, 2010, Lian et al, 2010. Natural infections of waterfowls are susceptible at all ages and there reported that in young ducks from 7 days of age (El-Samadony et al, 2013, El-Tholoth et al, 2019) and infected with direct or indirect contact and developed clinical manifestation within 3 to 7 days after infection (Wang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Duck Plague Virus for Determination of TCID<sub>50</sub>

Jahan

Rahman

Ahmed

et al. 2021

Res. Agric. Livest. Fish.

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Duck plague is an enveloped DNA virus that belongs to the Anatid Herpes Virus; the Herpesviridae family is an acute and highly infectious duck, geese, and swan disease that causes tremendous economic losses of duck rearing in Bangladesh and other duck rearing countries. Therefore, we decided to isolate duck plague virus from recent fields’ outbreaks area and performed molecular detection and phylogenetic analysis to find out the similarities between our findings and other isolates around the world. Visceral organs of 13 suspected ducks from recent outbreaks area were collected by post-mortem examination for inoculum preparation. Several passages were performed to harvest into 9-11 old embryonated eggs Chorioallantoic membrane (CAM) route and duck embryo fibroblast (DEF) primary cell culture. DNA polymerase (446bp) and DNA polymerase (UL, 602bp) genes were used for molecular detection by Polymerase chain reaction (PCR). Pathogenicity was done with duckling and TCID50 on DEF. Molecular characterization was performed from extracted DNA of duckling and 2 Positive PCR products were partially sequenced for phylogenetic analysis of their origin and nucleotide variations. Sequenced data was analyzed to reveal genetic relationships among constructed phylogenetic tree for understanding potential transmission with origin of virus and data was then submitted to gene bank and got accession number for DPV-BR1-MN937272 and DPV-BR-2-MN937273. Among 13 samples, 4(30.77%) were found positive by PCR using DNA polymerase at 446 bp and UL at 602 bp gene. Chorioallantoic membrane (CAM) was observed hemorrhagic after 72 days and duck embryo fibroblast (DEF) become round as showed cytopathic characteristics after 48h of infection .Duckling showed that isolated virus was highly pathogenic as characteristics signs of post-mortem examination. Therefore, this has found that recent isolates have similarity with Bangladesh, India and China isolates. Moreover, TCID50 has confirmed the isolates have accepted titer to be a vaccine strain. Res. Agric., Livest. Fish.8(1): 125-133, April 2021

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“…In India, the disease outbreak was recorded in West Bengal 4 , Karnataka 5 , UĴ ar Pradesh 6 , Kerala 7 , Tamil Nadu [8][9] and Assam 10 . More than 25 outbreaks were recorded in various districts of Assam state in India during the period from August 2012 to December 2015 11 . The present study reports the occurrence of duck viral enteritis outbreak in the Cauvery delta region of Tamil Nadu.…”

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confidence: 99%

Outbreak of duck viral enteritis in the Cauvery delta region of Tamil Nadu

Thilagavathi,

Selvaraj,

Jaisree

et al. 2024

Indian Journal of Veterinary Pathology

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Necropsy was conducted on three ducks, which were reared in the Cauvery delta region in a fl ock containing 3000 birds and were not vaccinated. Tissue samples like intestine, oesophagus, spleen, kidney and liver were collected for polymerase chain reaction (PCR) and histopathology. Grossly, oral cavity and oesophageal mucosa showed adhesion of diphtheritic membrane. Liver revealed multiple white necrotic foci. Epicardium showed petechial haemorrhages. Intestine mucosa revealed annular band haemorrhage, multiple petechiael haemorrhages, annular band with yellowish diphtheritic adhesion and multiple diphtheritic buĴ on ulcers of 2-3 mm diameter. PCR showed amplifi cation produces band size at 446 bp using specifi c primer showed positive for duck viral enteritis virus. Histopathologically, intestinal mucosa revealed fi brinous exudate with desquamated epithelial cells, clumps of bacteria and infl ammatory cells. Based on the gross lesions, PCR and histopathological lesions, the present case was confi rmed as duck viral enteritis.

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Detection and isolation of Duck Plague virus from field outbreaks in Assam, India

Cited by 3 publications

References 20 publications

Molecular characterization of duck plague virus from selected Haor areas of Bangladesh

Molecular characterization of duck plague virus from selected Haor areas of Bangladesh

Molecular Characterization of Duck Plague Virus for Determination of TCID<sub>50</sub>

Outbreak of duck viral enteritis in the Cauvery delta region of Tamil Nadu

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