Detection and Isolation of Winter Dysentery Bovine Coronavirus Circulat in Korea during 2002-2004

Abstract

ABSTRACT. Although winter dysentery (WD) has been suspected to occur frequently in Korea, to date the exact epidemiology of WD has remained unknown. Therefore, we investigated the causative agents of WD by using electron microscopy, ELISA, RT-PCR, and nested PCR from 97 fecal samples of 32 WD-affected herds collected from 8 provinces during [2002][2003][2004]. The bovine coronavirus (BoCV) was consistently detected in all herds with WD. Of other pathogens, only coccidian oocyts were inconsistently but concurrently detected with BoCV. Ten isolates were identified as BoCV by immune electron microscope, immunofluorescent test and ELISA with antiserum to BoCV, and RT-PCR. From these results, it is concluded that WD caused by BoCV occurred in relatively high frequency and was widespread in Korea. The results provide important epidemiological data for the control and establishment of a surveillance system for WD in Korea. KEY WORDS: bovine coronavirus, Korea, winter dysentery prevalence.

Coronavirus infection in intensively managed cattle with respiratory disease

“…However, in other cases, isolates of BoCV with >98% phylogenetic similarity have been identified in enteric and respiratory disease and an isolate of BCoV from a case of winter dysentery demonstrated both respiratory and enteric tropism after experimental infection . Phylogenetic differences between BCoV isolates from different geographical locations are greater than the differences between isolates from different clinical syndromes . Further characterisation of BCoV isolates from Australian cattle is required and the potential for variation in the genome sequence needs to be considered when interpreting qRT‐PCR assays.…”

Tracing the transmission of bovine coronavirus infections in cattle herds based on S gene diversity

“…The sequence of the S2 subunit is more conserved and this subunit is responsible for cell membrane fusion activity (Yoo et al, 1991). The S1 gene has been exploited as a target to study the molecular epidemiology of BCoV infection (Hasoksuz et al, 2002a;Jeong et al, 2005;Liu et al, 2006).…”

Molecular analysis of S gene of spike glycoprotein of winter dysentery bovine coronavirus circulated in Korea during 2002–2003

“…Ten WD strains, KWD1-KWD10, were originally isolated in the G clone of human rectal tumor cells (HRT-18G) from fecal samples of adult dairy or beef cows, with WD in South Korea, and identified as a BCoV by immunoelectron microscopy, ELISA with BCoV-specific antisera and monoclonal antibodies, and RT-PCR specific for the part of BCoV N gene (Jeong et al, 2004). RNA was extracted from the supernatant fluids of HRT-18G cells infected with 10 KWD strains, based on the acid quanidinium-phenol-chloroform RNA extraction method as described by Cho et al (2001a).…”