Detection and molecular characterization of enteric viruses in children with acute gastroenteritis in Northern Italy

Biscaro, Valeria; Piccinelli, Giorgio; Gargiulo, Franco; Ianiro, Giovanni; Caruso, Arnaldo; Caccuri, Francesca; Francesco, Maria Antonia De

doi:10.1016/j.meegid.2018.02.011

Cited by 61 publications

(58 citation statements)

References 51 publications

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“…This disparity amongst studies may be due to the number of viruses analyzed, viral detection methods, season of sampling, and sample size. The most common coinfections were between RVA and HAstV which is in contrast with reports of Biscaro et al, Ferreira et al, and Ouédraogo et al who had RVA and HAdV as the most prevalent. Pang and Vesikari previously reported the rate of HAstV and RVA coinfections among gastroenteritis in children to be extremely low (10 of 1528 or 0.006%).…”

Section: Discussioncontrasting

confidence: 99%

“…A relatively low frequency of coinfection (9.7%) was observed in this study, which is lower than previously reported in Burkina Faso (35.7%) and Brazil (14%), but higher compared with studies from China (5.2%), Northern Italy (2.3%), and Nigeria (7.1%) . This disparity amongst studies may be due to the number of viruses analyzed, viral detection methods, season of sampling, and sample size.…”

Section: Discussioncontrasting

confidence: 93%

“…RVA was detected in 16.6% cases, which is lower to studies from Angola, Burkina Faso, Northern Italy, Enugu, and Edo States, Nigeria . Both NoV and HAdV in this study were found in 5.1% of children with gastroenteritis which is similar to that reported in Republic Democratic of Congo and North East, Nigeria but lower compared with studies in Albania, Burkina Faso, Northern Italy, and Ondo State, Nigeria . These differences might be due to viral detection methods used, seasonal variation and nonintroduction of RVA immunization.…”

Section: Discussionsupporting

confidence: 88%

“…Worldwide, RVA is responsible for many deaths, mainly among young children in Asia and Africa . RVA was detected in 16.6% cases, which is lower to studies from Angola, Burkina Faso, Northern Italy, Enugu, and Edo States, Nigeria . Both NoV and HAdV in this study were found in 5.1% of children with gastroenteritis which is similar to that reported in Republic Democratic of Congo and North East, Nigeria but lower compared with studies in Albania, Burkina Faso, Northern Italy, and Ondo State, Nigeria .…”

Section: Discussioncontrasting

confidence: 49%

“…Worldwide, the prevalence of enteric viruses (EVs) in children with acute gastroenteritis has been reported to range from 29.6% to 85.6% . Previous studies in Nigeria showed the different prevalence of EVs infection .…”

Section: Introductionmentioning

confidence: 99%

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Epidemiology of enteric viruses in children with gastroenteritis in Ogun State, Nigeria

Arowolo

Ayolabi

Lapinski

et al. 2019

Journal of Medical Virology

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Acute gastroenteritis (AGE) remains a global public health concern and Nigeria is one of the two countries accounting for 42% of global under‐5 deaths attributable to gastroenteritis. This study aimed to determine the prevalence, seasonality, and risk factors of enteric viruses (EVs) in children with AGE in Ogun State, Nigeria. Stool samples collected from children under‐5 from three different hospitals between February 2015 and April 2017 were analyzed using molecular methods for the presence of four EVs (group A rotavirus [RVA], norovirus [NoV], human astrovirus [HAstV], and human adenovirus [HAdV]). Among the 175 samples analyzed, 63 (36%) were positive for at least one EV. The most prevalent was HAstV (19.4%), followed by RVA (16.6%), NoV (5.1%), and HAdV (5.1%). Mixed infections were found in 17 cases. No significant association was observed with age, sex, and risk factors. Though not significant, EV prevalence was higher in the dry season. Positive cases (asides HAdV) had no correlation with temperature and/or humidity. This study provides information on the prevalence and seasonal fluctuations of EVs, which will be of value in the effective management of patients and control strategies for viral gastroenteritis in the country.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 93%

Section: Discussionsupporting

confidence: 88%

Section: Discussioncontrasting

confidence: 49%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Epidemiology of enteric viruses in children with gastroenteritis in Ogun State, Nigeria

Arowolo

Ayolabi

Lapinski

et al. 2019

Journal of Medical Virology

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Association Between Acute Gastroenteritis and Continuous Use of Proton Pump Inhibitors During Winter Periods of Highest Circulation of Enteric Viruses

et al. 2019

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IMPORTANCE An increased risk of acute bacterial enteric infections has been reported among patients receiving proton pump inhibitor (PPI) therapy. The risk of acute gastroenteritis (AGE) of viral origin associated with continuous PPI exposure has been less studied. OBJECTIVE To investigate the association between continuous PPI therapy and AGE occurrence during winter epidemic periods when the circulation of enteric viruses is the highest. DESIGN, SETTING, AND PARTICIPANTS A matched cohort study was performed using a prospectively collected drug dispensing database from a large panel of community pharmacies in continental France. All patients recorded in the database during the 2015 to 2016 winter season, with documented age, sex, and use of an identifiable regular panel pharmacy, were eligible for the study. Each patient exposed to continuous PPI therapy was matched to 3 unexposed patients, according to year of birth, sex, and identifiable regular panel pharmacy. Analyses were performed between January 2017 and December 2018. EXPOSURE Continuous PPI use during the 2015 to 2016 AGE winter epidemic. MAIN OUTCOMES AND MEASURES The occurrence of at least 1 AGE episode during the 2015 to 2016 AGE winter epidemic was the main outcome. Episodes of AGE were identified using a previously validated algorithm based on drug dispensing data. Relative risks of AGE were estimated using a multivariable log-binomial model adjusted for age, sex, and treatments for chronic conditions. RESULTS There were 233 596 patients receiving PPI therapy (median [interquartile range] age, 71 [62-81] years; 55.8% female) and 626 887 matched patients not receiving PPI therapy (median [interquartile range] age, 70 [61-80] years; 56.3% female) included in the analyses. At least 1 AGE epidemic episode was identified in 3131 patients (1.3%) receiving PPI therapy and in 4327 patients (0.7%) not receiving PPI therapy. The adjusted relative risk of AGE for those receiving PPI therapy was 1.81 (95% CI, 1.72-1.90) for all ages considered, 1.66 (95% CI, 1.54-1.80) among those aged 45 to 64 years, 2.19 (95% CI, 1.98-2.42) among those aged 65 to 74 years, and 1.98 (95% CI, 1.82-2.15) among those aged 75 years and older. CONCLUSIONS AND RELEVANCE Continuous PPI therapy was associated with an increased risk of developing AGE during periods of highest circulation of enteric viruses. These findings support the hypothesis that PPI use is associated with an increased risk of enteric viral infections.

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Prevalence, molecular characterization, and clinical features of human bocavirus in children under 5 years of age with acute gastroenteritis admitted to a specialized children's hospital in Iran: A cross‐sectional study

Kachooei,

Karbalaie Niya,

Khales

et al. 2023

Health Science Reports

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Background and AimsAlthough some reports have confirmed the role of human bocavirus (HBoV) in respiratory infections, the importance of this virus in causing acute gastroenteritis has not yet been proven. This study aimed to determine the molecular prevalence of HBoV in children under 5 years old with gastroenteritis and to compare the clinical symptoms of HBoV‐positive and ‐negative gastroenteritis cases.MethodsA total of 100 stool samples were collected from children with gastroenteritis hospitalized in a pediatric hospital in Tehran, Iran. Demographic and clinical data were collected from patients' medical records. Viral genomic DNA was extracted from stool samples and amplified using the PCR assay. Finally, sequencing was used to determine the genotype of HBoV.ResultsThe HBoV genome was detected in 14 samples (14%). The highest prevalence of HBoV was observed in the age range of 24–60 months (n = 5; 35.7%); However, no statistically significant relationship was observed between the prevalence of HBoV and age groups (p = 0.09). Nine (64.3%) and 5 (35.7%) HBoV‐positive cases were boys and girls, respectively (p = 0.45). Fever, vomiting, and heartache were seen in 5 (35.7%), 3 (21.4%), and 1 (7.1%) HBoV‐positive patients, respectively. Overall, no significant difference was observed in any of the investigated clinical manifestations between patients positive or negative for HBoV. Five HBoV‐positive samples were subjected to sequencing and all five sequenced samples were genotype 3.ConclusionHBoV infections can be considered a risk factor for causing at least a portion of acute gastroenteritis cases in children under 5 years of age.

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Detection and molecular characterization of enteric viruses in children with acute gastroenteritis in Northern Italy

Cited by 61 publications

References 51 publications

Epidemiology of enteric viruses in children with gastroenteritis in Ogun State, Nigeria

Epidemiology of enteric viruses in children with gastroenteritis in Ogun State, Nigeria

Association Between Acute Gastroenteritis and Continuous Use of Proton Pump Inhibitors During Winter Periods of Highest Circulation of Enteric Viruses

Prevalence, molecular characterization, and clinical features of human bocavirus in children under 5 years of age with acute gastroenteritis admitted to a specialized children's hospital in Iran: A cross‐sectional study

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