Detection and molecular characterization of chicken infectious anaemia virus in young chicks in Punjab region of north-western India

Mohandas, Sreekala S.; Gurpreet, K.; Dwivedi, P. N.

doi:10.1007/s42770-019-00160-9

Cited by 7 publications

(4 citation statements)

References 32 publications

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“…The molecular epidemiology of CAV had been previously described, and several genotypes of CAV had been determined by phylogenetic analysis ( 15 , 16 , 19 , 29 , 30 ). In this study, phylogenetic analysis using full-length CAV genomic sequences was performed according to previous studies.…”

Section: Discussionmentioning

confidence: 99%

Genomic Characterization of Chicken Anemia Virus in Broilers in Shandong Province, China, 2020–2021

Liu

Li²,

Yin

et al. 2022

Front. Vet. Sci.

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Chicken infectious anemia (CIA), caused by chicken anemia virus (CAV), is an immunosuppressive disease characterized by growth retardation, aplastic anemia, lymphoid depletion, and immunodepression in young chickens. In this study, 33 CAV strains were isolated from broilers in Shandong Province during 2020–2021. Phylogenetic analysis of full-length genome sequences showed that most CAV strains isolated in this study were scattered across different branches, but mainly clustered in two genotypes, indicating a certain regional characteristic. Analysis of VP1 protein identified several amino acid substitutions which were relevant with the virulence and virus spread efficiency. Interestingly, four putative DNA recombination events were detected in the genomes of novel isolated CAV strains. In summary, this study demonstrated a genomic diversity of CAV in broilers isolated in Shandong Province during 2020–2021, and provided information for the further study of CAV molecular epidemiology and viral evolution.

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Section: Discussionmentioning

confidence: 99%

Genomic Characterization of Chicken Anemia Virus in Broilers in Shandong Province, China, 2020–2021

Liu

Li²,

Yin

et al. 2022

Front. Vet. Sci.

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“…In addition, the VP2 protein is used as a target antigen to produce monoclonal antibodies. However, research on the VP2 gene is limited [27]. Therefore, a better understanding of this protein could aid in managing CAV infection more effectively.…”

Section: Discussionmentioning

confidence: 99%

“…Chicks below two to three weeks of age exhibit symptoms that include anemia, T Egyptian Journal of Veterinary Sciences https://ejvs.journals.ekb.eg/ stunted growth, lethargy, drooping wings, untidy feathers, paleness of the soft tissues, crest, wattles, and beak [10]. Additionally, blood loss (intramuscular and subcutaneous) and abnormalities of the thymus and bone marrow have been observed [11].…”

Section: Introductionmentioning

confidence: 99%

Genetic Differences and Pathogenicity of Chicken Anemia Virus Strains in Broiler’s Baby Chicks

Elsamadony,

Mekky,

Fedawy

et al. 2025

Egyptian Journal of Veterinary Sciences

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HIS STUDY aims to molecular characterization of the Chicken Anemia Virus (CAV) in young broilers in the Giza governorate, Egypt in 2021, assess the genetic diversity among the proliferating viruses, and vaccine strains, and evaluate their pathogenicity to improve viral management. Viral protein 2 (VP2) gene was amplified and subsequently conducted molecular-level characterization. In five out of the ten flocks sampled CAV was detected. Furthermore, the examination of the phylogenetic connections performed according to the sequences of the VP2 gene demonstrated the classification of the observed field strains within genogroups I, II, and IIIb. In silico analysis was performed on Cux-1 and the detected five strains to investigate the secondary structures of VP2 proteins. The findings indicated a significant resemblance in the distribution and quantity of α-helical and β-strand topologies. To assess the pathogenicity of the virus, two isolates from genotype III (MZ574096.1) and genotype II (MZ477000.1) were administered intramuscularly to sets of dayold, specific pathogen-free chicks. At 28 days following inoculation, the Red Blood Corpuscle count (X106µL) was (1.4 ±0.39 and 1.8± 0.12).

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“…Infection with CIAV can heighten the virulence of attenuated vaccines or diminish the efcacy of some vaccines, such as Marek's disease virus (MDV) vaccine, thus resulting in the inefectiveness of the vaccination [3,4]. Since its frst isolation in Japan, CIAV has been widely prevalent worldwide [5][6][7][8][9][10][11]. CIAV is able to spread in a vertical or horizontal manner, remaining resilient in chicken focks and posing a challenge to be eliminated, causing major fnancial losses to the global poultry breeding industry [12].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Identification, Sequence Analysis, and Pathogenicity of a CIAV Strain from Aegypius monachus

Yin

Tang²,

Hu³

et al. 2023

Transboundary and Emerging Diseases

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A cluster of vultures, artificially bred in a zoo in Shandong Province, China, displayed signs of emaciation and some even died, which raised the suspicion of an immunosuppressive pathogen. Upon conducting nucleic acid testing on the clinical samples, it was found that the CIAV was present in the tissue of dead vulture. In this study, samples of dead vulture tissue were used to isolate a chicken infectious anemia virus (CIAV) via the MDCC-MSB1 cell line, which was designated the SDTY2021-TJ strain. The full-length genomic sequence of SDTY2021-TJ was determined and analyzed in detail. The full genomic DNA of SDTY2021-TJ was found to be 2298 bp, with no omissions or additions in the coding region. The homology between the full-length genomic sequence of SDTY2021-TJ and the reference strain ranged from 95.5% (Del-Ros) to 98.7% (YN04). In comparison with the reference strain, the VP1 protein of SDTY2021-TJ contained a number of mutations. To assess the virulence of SDTY2021-TJ, one-day-old SPF chickens were inoculated with both high and low doses of the pathogen. The results demonstrated that SDTY2021-TJ had a considerable pathogenicity to SPF chickens, as the high-dose group caused a 50% mortality rate, and even the low-dose group caused a 30% mortality rate. The chickens infected with the disease exhibited paleness in the cockscomb and stunted growth, as well as a compromised response to NDV and AIV-H9 inactivated vaccine. Furthermore, histological observation revealed an atrophy and degeneration of the thymus. To the best of our knowledge, this is the first instance of CIAV being isolated from artificially bred Aegypius monachus, implying that wild birds may be involved in the transmission and spread of CIAV.

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Detection and molecular characterization of chicken infectious anaemia virus in young chicks in Punjab region of north-western India

Cited by 7 publications

References 32 publications

Genomic Characterization of Chicken Anemia Virus in Broilers in Shandong Province, China, 2020–2021

Genomic Characterization of Chicken Anemia Virus in Broilers in Shandong Province, China, 2020–2021

Genetic Differences and Pathogenicity of Chicken Anemia Virus Strains in Broiler’s Baby Chicks

Isolation, Identification, Sequence Analysis, and Pathogenicity of a CIAV Strain from Aegypius monachus

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