Detection and molecular characterization of the rice root-knot nematode Meloidogyne graminicola in Italy

Fanelli, Elena; Cotroneo, A.; Carisio, Loredana; Troccoli, Alberto; Grosso, Silvio; Boero, Cristina; Capriglia, Francesco; Luca, Francesca De

doi:10.1007/s10658-017-1196-7

Cited by 33 publications

(35 citation statements)

References 18 publications

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“…The chemical structures of the active compound were determined using nuclear magnetic resonance (NMR) analysis and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) analysis. 1 H nuclear magnetic resonance (NMR) and 13 C NMR spectra were acquired in MeOH with a Bruker AVANCE III HD600 spectrometer (Bruker Corporation, Faellanden, Switzerland) at 600 MHz for 1 H NMR spectra and 125 MHz for 13 C NMR spectra using tetramethylsilane as the internal standard. HR-ESI-MS analysis was performed using a Waters E2695 model ion trap mass spectrometer (Waters, Milford, MA, USA) [34].…”

Section: Isolation and Structural Determination Of Aspergillus Japonimentioning

confidence: 99%

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Nematicidal Evaluation and Active Compounds Isolation of Aspergillus japonicus ZW1 against Root-Knot Nematodes Meloidogyne incognita

Wang

et al. 2020

Agronomy

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The root-knot nematode is one of the most damaging plant-parasitic nematodes worldwide, and the ecofriendly alternative approach of biological control has been used to suppress nematode populations. Here the nematicidal activity of Aspergillus japonicus ZW1 fermentation filtrate against Meloidogyne incognita was evaluated in vitro and in greenhouse, and the effects of A. japonicus ZW1 fermentation filtrate on seed germination and the active compound of A. japonicus ZW1 fermentation filtrate were determined. The 2-week fermentation filtrate (2-WF) of A. japonicus ZW1 exhibited markedly inhibitory effects on egg hatching, and 5% 2-WF showed potential nematicidal activities on second-stage juveniles (J2s); the mortality of J2s was 100% after 24 h exposure. The internal contents of nematodes were degraded and remarkable protruded wrinkles were present on the body surface of J2s. The nematicidal activity of the fermentation was stable after boiling and was not affected by storage time. A germination assay revealed that 2-WF did not have a negative effect on the viability and germination of corn, wheat, rice, cowpeas, cucumbers, soybeans, or tomato seeds. The pot-grown study confirmed that a 20% fermentation broth solution significantly reduced root galls and egg numbers on tomatoes, and decreased galls and eggs by 47.3% and 51.8% respectively, over Czapek medium and water controls. The active compound from the A. japonicus ZW1 fermentation filtrate was isolated and identified as 1,5-Dimethyl Citrate hydrochloride ester on the basis of nuclear magnetic resonance (NMR) and LC-MS (liquid chromatograph-mass spectrometer) techniques. Thus, fermentation of A. japonicus ZW1 could be considered a potential new biological nematicide for the control of M. incognita.

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Section: Isolation and Structural Determination Of Aspergillus Japonimentioning

confidence: 99%

“…Root-knot nematodes (Meloidogyne spp.) are economically important worldwide pathogens causing considerable damage to many crops, including cucumbers, tomatoes, rice [1][2][3][4], and even cotton [5,6]. Meloidogyne incognita is an important species of root-knot nematodes worldwide due to its direct impact on crop yields [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Nematicidal Evaluation and Active Compounds Isolation of Aspergillus japonicus ZW1 against Root-Knot Nematodes Meloidogyne incognita

Wang

et al. 2020

Agronomy

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“…Root-knot nematodes (RKN) ( Meloidogyne species) are obligate sedentary parasites that cause considerable damage to crops by developing into plant roots. The rice nematode Meloidogyne graminicola is prevalent across Asian and Latin American paddy rice ( Oryza sativa L.) fields [ 1 , 2 ], and was recently reported in Italy [ 3 ]. Severe infections result in stunted root systems, which ultimately result in poor growth and crop production with substantial yield losses.…”

Section: Introductionmentioning

confidence: 99%

Analyses of the Root-Knot Nematode (Meloidogyne graminicola) Transcriptome during Host Infection Highlight Specific Gene Expression Profiling in Resistant Rice Plants

et al. 2020

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The plant-parasitic nematode Meloidogyne graminicola causes considerable damages to rice (Oryza sativa) culture. Resistance to M. graminicola in the related species Oryza glaberrima reduces root penetration by juveniles and stops further nematode development. M. graminicola genes expressed during O. sativa infection were previously characterized but no information is available about the molecular dialogue established with a resistant plant. We compared the M. graminicola transcriptomes of stage-two juveniles (J2s) before and during infection of susceptible or resistant rice. Among 36,121 M. graminicola genes surveyed, 367 were differentially expressed during infection of resistant or susceptible plants. Genes encoding cell wall-degrading enzymes, peptidases and neuropeptides were expressed for a longer time in resistant plants compared to susceptible plants. Conversely, genes related to nematode development were not activated in the resistant host. The majority of M. graminicola effector genes had similar expression patterns, whatever the host genotype. However, two venom allergen-like protein (VAP)-encoding genes were specifically induced in resistant plants and Mg-VAP1 silencing in J2s reduced their ability to colonize roots. This study highlighted that M. graminicola adapts its gene expression to the host susceptibility. Further investigation is required to assess the role of Mg-VAPs in the rice–nematode interaction.

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“…M. graminicola is widely distributed in many rice growing areas in South and Southeast Asia (Jain et al 2012 ). Although M. graminicola is considered a serious pest in the tropics (Jones et al 2013 ), it has recently been detected in rice fields in Italy (Fanelli et al 2017 ). This is the first report of this pest in temperate rice production.…”

Section: Introductionmentioning

confidence: 99%

QTL-seq reveals a major root-knot nematode resistance locus on chromosome 11 in rice (Oryza sativa L.)

et al. 2019

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The root-knot nematode Meloidogyne graminicola is a serious pest in rice affecting production in many rice growing areas. Natural host resistance is an attractive control strategy because the speed of the parasite’s life cycle and the broad host range it attacks make other control measures challenging. Although resistance has been found in the domesticated African rice Oryza glaberrima and the wild rice species O. longistaminata, the introgression of resistance genes to Asian rice O. sativa is challenging. Resistance due to a major gene in O. sativa would greatly aid breeding. Recently two accessions resistant to M. graminicola have been identified in a screen of 332 diverse O. sativa cultivars. In this study, these two resistant cultivars, LD 24 (an indica from Sri Lanka) and Khao Pahk Maw (an aus from Thailand), were crossed with a moderately susceptible cultivar, Vialone Nano (a temperate japonica from Italy). Approximately 175 F 2 progeny of both populations were screened for susceptibility to M. graminicola infection. Between 20 and 23 individuals with highest and lowest galls per plants were pooled to make susceptible and resistant bulks which were sequenced to conduct bulked segregant analysis using the QTL-seq method. This revealed a nematode resistance locus from 23 Mbp to the bottom of rice chromosome 11 in both crosses suggesting a rare introgression of the same locus is responsible for resistance in both cultivars. While this information can be used in marker-assisted breeding, analysis of available SNP data revealed candidate loci and genes worthy of further investigation for gene identification. Electronic supplementary material The online version of this article (10.1007/s10681-019-2427-0) contains supplementary material, which is available to authorized users.

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Detection and molecular characterization of the rice root-knot nematode Meloidogyne graminicola in Italy

Cited by 33 publications

References 18 publications

Nematicidal Evaluation and Active Compounds Isolation of Aspergillus japonicus ZW1 against Root-Knot Nematodes Meloidogyne incognita

Nematicidal Evaluation and Active Compounds Isolation of Aspergillus japonicus ZW1 against Root-Knot Nematodes Meloidogyne incognita

Analyses of the Root-Knot Nematode (Meloidogyne graminicola) Transcriptome during Host Infection Highlight Specific Gene Expression Profiling in Resistant Rice Plants

QTL-seq reveals a major root-knot nematode resistance locus on chromosome 11 in rice (Oryza sativa L.)

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