Detection and molecular characterization of Livestock-Associated MRSA in raw meat on retail sale in North West England

Pichon, Bruno; Wilkinson, Hayley; Doumith, Michel; Hill, Robert L.; McLauchlin, Jim; Kearns, Angela

doi:10.1111/lam.12709

Cited by 40 publications

(31 citation statements)

References 29 publications

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“…Based on CLSI subclasses criteria, 27 out of 29 MRSA strains (93.1%) were multidrug-resistant MRSA strains ( Table 5 ). In several studies ( Abdalrahman et al., 2015 ; Fox et al, 2017 ; Li et al, 2017 ; Normanno et al, 2015 ), a large proportion of MRSA strains isolated from meat were also revealed to be multidrug-resistant. Ge et al (2017) reported that multidrug-resistance (MDR) rates in MRSA (37.2%, 29/78) were higher than those of methicillin-susceptible Staphylococcus aureus (MSSA) (8.2%, 78/954).…”

Section: Resultsmentioning

confidence: 99%

Prevalence and Characteristics of Antimicrobial-Resistant Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus from Retail Meat in Korea

Kim¹,

Kim²,

Kim³

et al. 2020

Food Sci Anim Resour

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This study was to investigate the prevalence and characteristics of antimicrobial-resistant Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) from 4,264 retail meat samples including beef, pork, and chicken in Korea between 2013 and 2018. A broth microdilution antimicrobial susceptibility testing was performed for S. aureus . Molecular typing by multilocus sequence typing (MLST), spa typing, and pulsed-field gel electrophoresis (PFGE), was performed on mecA -positive S. aureus strain. S. aureus was isolated at a rate of 18.2% (777/4,264), of which MRSA comprised 0.7% (29 strains). MLST analysis showed that 11 out of the 29 MRSA isolates were predominantly sequence type (ST) 398 (37.9%). In addition, ST72, ST692, ST188, ST9, and ST630 were identified in the MRSA isolates. The spa typing results were classified into 11 types and showed a high correlation with MLST. The antimicrobial resistance assays revealed that MRSA showed 100% resistance to cefoxitin and penicillin. In addition, resistance to tetracycline (62.1%), clindamycin (55.2%), and erythromycin (55.2%) was relatively high; 27 of the 29 MRSA isolates exhibited multidrug resistance. PFGE analysis of the 18 strains excluding the 11 ST398 strains exhibited a maximum of 100% homology and a minimum of 64.0% homology. Among these, three pairs of isolates showed 100% homology in PFGE; these results were consistent with the MLST and spa typing results. Identification of MRSA at the final consumption stage has potential risks, suggesting that continuous monitoring of retail meat products is required.

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Section: Resultsmentioning

confidence: 99%

Prevalence and Characteristics of Antimicrobial-Resistant Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus from Retail Meat in Korea

Kim¹,

Kim²,

Kim³

et al. 2020

Food Sci Anim Resour

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“…In a single-hospital study in Germany, admission screening at one hospital identified 25% of MRSA isolates to be of livestock-associated type, yet LA-MRSA amounted to only 7% of MRSA isolates from clinical sample submissions (Köck et al, 2011). The identification since 2005 of widespread LA-MRSA colonization of pig herds in many countries with intensive pig production (EFSA, 2009;Lassok & Tenhagen, 2013), plus in retail pork and other meats (Fox et al, 2017;Lassok & Tenhagen, 2013;Verhegghe et al, 2016), has led to a focus on characterization and control of this AMR organism, plus substantial interest in the media and among a general public familiar with MRSA from healthcare and community sources.…”

Section: Current Issues For Amr In Livestock Bacteriamentioning

confidence: 99%

Antimicrobial Resistance on Farms: A Review Including Biosecurity and the Potential Role of Disinfectants in Resistance Selection

Davies

Wales

2019

Comp Rev Food Sci Food Safe

124

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Resistance to therapeutic antimicrobial agents is recognized as a growing problem for both human and veterinary medicine, and the need to address the issue in both of these linked domains is a current priority in public policy. Efforts to limit antimicrobial resistance (AMR) on farms have so far focused on control of the supply and use of antimicrobial drugs, plus husbandry measures to reduce infectious disease. In the United Kingdom and some other countries, substantial progress has been made recently against targets on agricultural antimicrobial drug use. However, evidence suggests that resistant pathogenic and commensal bacteria can persist and spread within and between premises despite declining or zero antimicrobial drug use. Reasons for this are likely complex and varied but may include: bacterial adaptations to ameliorate fitness costs associated with maintenance and replication of resistance genes and associated proteins, horizontal transmission of genetic resistance determinants between bacteria, physical transfer of bacteria via movement (of animals, workers, and equipment), ineffective cleaning and disinfection, and co‐selection of resistance to certain drugs by use of other antimicrobials, heavy metals, or biocides. Areas of particular concern for public health include extended‐spectrum cephalosporinases and fluoroquinolone resistance among Enterobacteriaceae, livestock‐associated methicillin‐resistant Staphylococcus aureus, and the emergence of transmissible colistin resistance. Aspects of biosecurity have repeatedly been identified as risk factors for the presence of AMR on farm premises, but there are large gaps in our understanding of the most important risk factors and the most effective interventions. The present review aims to summarize the present state of knowledge in this area, from a European perspective.

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“…Despite the recognition of CC398 LA‐MRSA in neighbouring mainland Europe its epidemiology has been less well‐studied in the United Kingdom. Nonetheless, small numbers of CC398 MRSA isolates have been found, largely since 2012, from bovine milk (Paterson et al ), pigs (Hartley et al ; Hall et al ), poultry (Anonymous ), retail pork and poultry meat (Hadjirin et al ; Fox et al ), horses (Loeffler et al ; Bortolami et al ) and humans (Ward et al ; Harrison et al ; Kinross et al ) in the UK. Yet while clearly present in the UK and spread among different host species few data are available on the prevalence of CC398 MRSA in the UK human population.…”

Section: Introductionmentioning

confidence: 99%

Low prevalence of livestock‐associated methicillin‐resistantStaphylococcus aureusclonal complex 398 andmecCMRSA among human isolates in North‐West England

Paterson

2020

J Appl Microbiol

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Aims Livestock‐associated methicillin‐resistant Staphylococcus aureus (LA‐MRSA) is an important public health problem in many countries. Despite reports of such isolates being found in both animals and humans in the United Kingdom few data are available on the prevalence in humans of such isolates. A prevalence study was therefore undertaken in the north‐west of England. Methods and Results One thousand two hundred and forty‐two human MRSA isolates collected during 2015 were screened by PCR to detect two of the major forms of LA‐MRSA: clonal complex (CC)398 and mecC MRSA. Isolates identified were further characterized by antimicrobial susceptibility testing and whole‐genome sequencing using HiSeq technology. A single mecC MRSA isolate and three CC398 LA‐MRSA were identified among the isolates screened. All four isolates were from MRSA screening. A phylogenetic analysis, including previously sequenced isolates from the United Kingdom, provided strong evidence for the genomic and epidemiological linkage between a pair of animal and human isolates of CC398 LA‐MRSA in England. These data are indicative of animal and humans isolates of CC398 LA‐MRSA being involved in the same transmission network and is the first demonstration of such closely linked animal and human isolates of this lineage in the UK. Conclusions The study indicates there is a low prevalence of CC398 LA‐MRSA and mecC MRSA among MRSA isolates in the sampled population. Significance and Impact of the Study While the study demonstrates that LA‐MRSA were rare among human MRSA isolates in the sampled population, the data provide a baseline for the future surveillance of what is a significant public health challenge in some regions. The demonstration of linked human and animal isolates of CC398 LA‐MRSA, supported by genomic and epidemiological data, reinforces the need for such surveillance and for continued awareness of the risks that LA‐MRSA may pose in the UK.

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Detection and molecular characterization of Livestock-Associated MRSA in raw meat on retail sale in North West England

Cited by 40 publications

References 29 publications

Prevalence and Characteristics of Antimicrobial-Resistant Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus from Retail Meat in Korea

Prevalence and Characteristics of Antimicrobial-Resistant Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus from Retail Meat in Korea

Antimicrobial Resistance on Farms: A Review Including Biosecurity and the Potential Role of Disinfectants in Resistance Selection

Low prevalence of livestock‐associated methicillin‐resistantStaphylococcus aureusclonal complex 398 andmecCMRSA among human isolates in North‐West England

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