Detection and molecular characterization of Lymphocystivirus in Brazilian ornamental fish

Maganha, Samara Rita de Lucca; Cardoso, Pedro Henrique Magalhães; Balian, Simone de Carvalho; Almeida-Queiroz, Sabrina Ribeiro de; Fernandes, Andrezza Maria; Sousa, Ricardo Luiz Moro de

doi:10.1007/s42770-019-00196-x

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…The MCP gene is highly conserved in the family of Iridoviridae and has been used for phylogenetic and evolutionary analyses (Hossain et al, 2008;Maganha, 2020). The alignment analysis on the MCP using BLAST showed a high nucleotide sequence identity to 51 entries of Lymphocystis disease virus MCP genes.…”

Section: Clinical Signsmentioning

confidence: 99%

“…Lymphocystis disease causes severe economic losses in the aquaculture and ornamental fish industries. Affected fish has low market value due to disease-associated anemia, the appearance of external lesions, reduction in the growth rate, high susceptibility to secondary bacterial infections, and high rates of cannibalism (Hick et al, 2016;Maganha, 2020) Detection and genotype determination of lymphocystis ..... (Murwantoko) LCDV is a large iridovirus with virions of heterologous size characterized by an icosahedral capsid approximately 200-230 nm in diameter, a double-layered capsid with an outer envelope and a fringe of fibril-like external protrusions (Chinchar et al, 2017). LCDV is distinguished from other iridoviruses by a genomic GC content of 29% compared with 48-55% (Hick et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Phylogenetic and evolutionary analyses of the family Iridoviridae are mainly based on the highly conserved major capsid protein (MCP) gene (Hossain et al, 2008;Maganha, 2020). Several genotypes were present in the genus Lymphocystivirus, based on MCP gene sequence and the pathogenicity of lymphocystiviruses: genotype I (consisting of LCDV-1); genotype II (consisting of Japanese flounder isolates and sea bass isolate); genotype III (consisting of rockfish isolates) (Kitamura et al, 2006); genotype IV (for the sea bass isolate), genotype V (for painted glass fish isolates) and genotype VI (for gourami isolates) (Hossain et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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DETECTION AND GENOTYPE DETERMINATION OF LYMPHOCYSTIS DISEASE VIRUS FROM ORANGE CLOWNFISH Amphiprion percula IN BATAM

Murwantoko¹,

Wijayanti²,

Agustatik³

et al. 2022

Indonesian Aquaculture Journal

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The orange clownfish, Amphiprion percula, is a popular fish in the global marine ornamental trade. In March 2021, several orange clown fish at Batam Mariculture Development Center exhibited lethargic behavior with wart-like nodules on the fins suggesting a viral disease infection. This study aimed to detect the suspected viral disease agent from the clownfish and determine its genotype based on the major capsid protein gene. The fish tissues with wart-like nodules were collected and fixed in 96% ethanol. The DNA was extracted from the tissues and used as the template for the amplification of the major capsid protein (MCP) and myristylated membrane protein (MMP) genes using polymerase chain reaction (PCR). The nucleotide sequences of the PCR products were analyzed for their homology using the Basic Local Alignment Search Tool (BLAST). Multiple alignments of the amino acid sequence of MCP were performed using MEGA-X to determine the genotype. The PCR amplification produced the expected bands for detecting MCP, MMP and DNAPol genes. These results indicated the presence of Lymphocystis disease virus (LCDV), designated as LCDV-Oc-Btm. The sequencing of MCP and MMP genes produced the 1221 and 407 nucleotides, respectively. The BLAST analysis showed the highest identity was obtained with the species of LCDV-1 (LCDV-Sa strain) at 91.04 % and 88.19 % for MCP and MMP, respectively. The UPGMA phylogenic tree showed LCDV-Oc-Btm differs from the existing genotype and can be assigned as a new genotype. This study concludes that LCDVOc-Btm is a novel species of lymphocystis disease virus.

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Section: Clinical Signsmentioning

confidence: 99%