DETECTION AND PREVALENCE OF METHICILLIN AND VANCOMYCIN   RESISTANT Staphylococcus aureus AMONG CLINICAL ISOLATES IN ESUTH, ENUGU STATE

Okoye, Ebele Linda; Omeje, Munachimso Janefrances; Ugwuoji, Emmanuel Tobechukwu

doi:10.54117/jcbr.v2i2.13

Cited by 3 publications

(2 citation statements)

References 39 publications

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“…When the MDRI index is greater than 0.2, it indicates that the organisms were isolated in an environment where antibiotics are misused. Additionally, a signi cant portion of the bacterial isolates have been exposed to multiple antibiotics and have subsequently developed resistance to these antibiotics [31,54].The results indicate that this is the case in this study.…”

Section: Discussionsupporting

confidence: 55%

Multidrug Resistance Tracing by Plasmid Profile Analysis and the Curing of Bacteria from Different Clinical Specimens

Okoye

Kemakolam

Ugwuoji

et al. 2022

Preprint

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Human-pathogenic bacteria resistant to one or multiple antibiotics have dramatically increased worldwide in the past decades. These bacteria possess great danger, have become a global issue and it is now impossible to avoid developing strategies for the restoration of treatment options against infections caused by them. This research aims at profiling plasmids of multidrug-resistant bacteria from various clinical specimens such as ear exudate, sputum, urethral swab, wound swab, urine from the catheter, urine, nasal swab, high vaginal swab, stool, eye swab and blood at Chukwuemeka Odumegwu Ojukwu University Teaching Hospital Awka, Anambra State, Nigeria. Our investigation used the Agar diffusion method for susceptibility tests and identification of multidrug-resistant bacteria before plasmid extraction and gel electrophoresis. A plasmid curing test was performed with 10% Sodium Dodecyl Sulphate. Of the 860 bacteria whose resistance profile was determined, only 42 were multidrug-resistant. These bacteria include Pseudomonas aeruginosa 16 (38.10%), Staphylococcus aureus 12 (28.57%), Escherichia coli 9 (21.43%), and Klebsiella pneumoniae 5(11.90%). The molecular weight of their plasmids ranges between 20.884kbp and 187.50kbp. As indicated by the plasmid bands, some bacteria had similar molecular weight while others had no plasmid. The bacterial pattern of the post-curing sensitivity test showed that the bacteria with plasmid bands were cured as they became susceptible to the drugs they were previously resistant to, while the bacteria without plasmid bands remained resistant to the antibiotics. This implies that the latter’s multidrug resistance is non-plasmid mediated. Our analyses highlight the relationship between plasmids and multidrug resistance as well as the role of plasmids in the transmission of drug resistance across bacteria. Thus, in order to lessen the burden that multidrug-resistant bacteria cause and to improve bacterial infections treatments, there should be continued surveillance and periodic research on antibiotic resistance patterns of bacteria from various clinical settings.

show abstract

Section: Discussionsupporting

confidence: 55%

Multidrug Resistance Tracing by Plasmid Profile Analysis and the Curing of Bacteria from Different Clinical Specimens

Okoye

Kemakolam

Ugwuoji

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…When the MDRI index is greater than 0.2, it indicates that the organisms were isolated in an environment where antibiotics are misused. Additionally, a significant portion of the bacterial isolates have been exposed to multiple antibiotics and have subsequently developed resistance to these antibiotics [31,54]. The results indicate that this is the case in this study.…”

Section: Discussionsupporting

confidence: 54%

Multidrug Resistance Tracing by Plasmid Profile Analysis and the Curing of Bacteria from Different Clinical Specimens

Okoye

Kemakolam

Ugwuoji

et al. 2022

Advanced Gut & Microbiome Research

Self Cite

View full text Add to dashboard Cite

Human-pathogenic bacteria resistant to one or multiple antibiotics have dramatically increased worldwide in the past decades. These bacteria possess great danger, have become a global issue, and it is now impossible to avoid developing strategies for the restoration of treatment options against infections caused by them. This research aims at profiling plasmids of multidrug-resistant bacteria from various clinical specimens such as ear exudate, sputum, urethral swab, wound swab, urine from the catheter, urine, nasal swab, high vaginal swab, stool, eye swab, and blood at Chukwuemeka Odumegwu Ojukwu University Teaching Hospital, Awka, Anambra State, Nigeria. Our investigation used the agar diffusion method for susceptibility tests and identification of multidrug-resistant bacteria before plasmid extraction and gel electrophoresis. A plasmid curing test was performed with 10% sodium dodecyl sulphate. Of the 860 bacteria whose resistance profile was determined, only 42 were multidrug-resistant. These bacteria include Pseudomonas aeruginosa 16 (38.10%), Staphylococcus aureus 12 (28.57%), Escherichia coli 9 (21.43%), and Klebsiella pneumoniae 5(11.90%). The molecular weight of their plasmids ranges between 20.884 kbp and 187.50 kbp. As indicated by the plasmid bands, some bacteria had similar molecular weight while others had no plasmid. The bacterial pattern of the postcuring sensitivity test showed that the bacteria with plasmid bands were cured as they became susceptible to the drugs they were previously resistant to, while the bacteria without plasmid bands remained resistant to the antibiotics. This implies that the latter’s multidrug resistance is nonplasmid mediated. Our analyses highlight the relationship between plasmids and multidrug resistance as well as the role of plasmids in the transmission of drug resistance across bacteria. Thus, in order to lessen the burden that multidrug-resistant bacteria cause and to improve bacterial infections treatments, there should be continued surveillance and periodic research on antibiotic resistance patterns of bacteria from various clinical settings.

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Methicillin and vancomycin-resistant Staphylococcus aureus and associated risk factors among patients with wound infection in East Wallaga Zone, Western Ethiopia

Abebe,

Alemkere,

Ayele

2024

Infection Prevention in Practice

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DETECTION AND PREVALENCE OF METHICILLIN AND VANCOMYCIN RESISTANT Staphylococcus aureus AMONG CLINICAL ISOLATES IN ESUTH, ENUGU STATE

Cited by 3 publications

References 39 publications

Multidrug Resistance Tracing by Plasmid Profile Analysis and the Curing of Bacteria from Different Clinical Specimens

Multidrug Resistance Tracing by Plasmid Profile Analysis and the Curing of Bacteria from Different Clinical Specimens

Multidrug Resistance Tracing by Plasmid Profile Analysis and the Curing of Bacteria from Different Clinical Specimens

Methicillin and vancomycin-resistant Staphylococcus aureus and associated risk factors among patients with wound infection in East Wallaga Zone, Western Ethiopia

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