Detection and Quantification of 4-Methyliniidazole in Cola by Matrix-assisted Laser Desorption Ionization Mass Spectrometry with Fe2O3 Nanoparticles on Zeolite

Abstract

Food additives generally used in carbonated drinks, such as 4-methylimidazole (4MI), caffeine (Caf ), citric acid (CA), and aspartame (Apm), were measured by matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) using nanometer-sized particles of iron oxide (Fe2O3 NPs). The quantification of 4MI in Coca Cola (C-cola) was carried out. In order to improve the reproducibility of the peak intensities, Fe2O3 NPs loaded on ZSM5 zeolite were used as the matrix for quantification. By using 2-ethylimidazole (2EI) as the internal standard, the amount of 4MI in C-cola was determined to range from 88 to 65 μg/355 mL. The results agree with the published value (approx. 72 μg/355 mL). It was found that MALDI using Fe2O3 was applicable to the quantification of 4MI in C-cola.

The Effect of Oxidative Debris on the Laser Desorption/Ionization Efficiency of Graphene Oxide Derivatives for Mass Spectrometric Analysis of Small Molecules and Synthetic Polymers

“…2 Nanomaterials have been extensively investigated as alternatives to organic matrices for matrix-free laser desorption/ionization time-offlight mass spectrometry (LDI-TOF-MS) to avoid issues related to matrix interference. 3,4 Among the nanomaterials developed for this purpose, carbon nanomaterials including carbon dots, carbon nanotubes, and graphene derivatives are the most promising candidates for LDI-TOF-MS applications. 5 Especially, graphene oxide (GO) derivatives show great promise because they are processable in aqueous solution and exhibit an amphiphilic surface, tailorable surface functional groups, and high photo-thermal conversion efficiency, all of which are required for an efficient and functional LDI-TOF-MS platform.…”

Laser Mass Spectrometry

“…By using MALDI-TOFMS, Fujii et al measured food additives generally used in carbonated drinks, such as 4-methyimidazole, caffeine, citric acid, and aspartame, using nanometer-sized particles of iron oxide (Fe2O3) as the matrix. 7 Chen et al reported on the characteristic mass fragmentations of phenethylamine/ tryptamine standards. 8 Shimma's group applied MALDI imaging mass spectrometry (MALDI-IMS) to the visualization method for imidacloprid in Drosophila melanogaster 9 and asparaptine in asparagus.…”