Detection and quantification of hepatopancreatic parvovirus in penaeid shrimp by real-time PCR assay

Liu, Tianqi; Yang, Bing; Song, Xiaoling; Wang, Xiuhua; Yuan, Yue; Liu, Li; Huang, Jie

doi:10.1016/j.jip.2013.09.007

Cited by 6 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, real-time PCR requires less time and labor than conventional PCR. Therefore, real-time PCR assays are promising tools for detecting pathogen gDNA from biological fluids such as blood, urine, and sputum [18][19][20][21][22][23].…”

Section: Discussionmentioning

confidence: 99%

Development of Real-time PCR Assays for Detection ofDirofilaria immitisfrom Infected Dog Blood

Kim²,

Jun

et al. 2016

Korean J Clin Lab Sci.

View full text Add to dashboard Cite

Dirofilaria immitis is a filarial nematode parasite that causes cardiopulmonary dirofilariasis in dogs. The purpose of this study was the development of real-time PCR assays for efficient detection of D. immitis. The D. immitis-specific primers confirmed in our previous study and a newly designed TaqMan probe were used for quantitative diagnostics. First, SYBR Green real-time PCR was performed using the specific primers and serially diluted genomic DNA or plasmid DNA, and melting curve analyses were performed after amplification. The melting curve showed one specific peak in each of the genomic and plasmid DNA reactions, suggesting that the primers specifically amplify the D. immitis cytochrome c oxidase subunit I gene. Comparison of SYBR Green and TaqMan real-time PCR using serially diluted plasmid DNA showed higher efficiency and specificity with TaqMan real-time PCR. The real-time PCR assays developed in this study will provide improved diagnostic methods to overcome the limitations of conventional diagnostic tools and facilitate more rapid and accurate diagnoses.

show abstract

Section: Discussionmentioning

confidence: 99%

Development of Real-time PCR Assays for Detection ofDirofilaria immitisfrom Infected Dog Blood

Kim²,

Jun

et al. 2016

Korean J Clin Lab Sci.

View full text Add to dashboard Cite

show abstract

“…For the challenge test, specific-pathogen-free (SPF) juvenile P. vannamei shrimps were collected from Haixingnong Shrimp Breeding Northern Base of BLUMP Seed Industry Technology Co., Ltd. in Weifang city of Shandong Province. The juvenile shrimps were tested to be free of WSSV, IHHNV, Vp AHPND , EHP, YHV, and HPV in the PCR or RT-PCR assays recommended by the OIE Aquatic Manual ( OIE, 2019 ) and previous reports ( Liu et al, 2013 ; Tang et al, 2015 ).…”

Section: Methodsmentioning

confidence: 99%

Investigation of Pathogenic Mechanism of Covert Mortality Nodavirus Infection in Penaeus vannamei

et al. 2022

View full text Add to dashboard Cite

Viral covert mortality disease (VCMD), also known as running mortality syndrome (RMS), is caused by covert mortality nodavirus (CMNV) and has impacted the shrimp farming industry in Asia and Latin America in recent years. The pathogenic mechanism of CMNV infecting Penaeus vannamei was investigated in this study. In the naturally infected shrimp, histopathological and in situ hybridization (ISH) analysis verified that CMNV infection and severe cellar structural damage occurred in almost all cells of the ommatidium. Under transmission electron microscopic (TEM), vacuolation and necrosis, together with numerous CMNV-like particles, could be observed in the cytoplasm of most cell types of the ommatidium. The challenge test showed that a low CMNV infectious dose caused cumulative mortality of 66.7 ± 6.7% and 33.3 ± 3.6% of shrimp in the 31-day outdoor and indoor farming trials, respectively. The shrimp in the infection group grew slower than those in the control group; the percentage of soft-shell individuals in the infection group (42.9%) was much higher than that of the control group (17.1%). The histopathological and ISH examinations of individuals artificially infected with CMNV revealed that severe cellar damage, including vacuolation, karyopyknosis, and structural failure, occurred not only in the cells of the refraction part of the ommatidium, but also in the cells of the nerve enrichment and hormone secretion zones. And the pathological damages were severe in the nerve cells of both the ventral nerve cord and segmental nerve of the pleopods. TEM examination revealed the ultrastructural pathological changes and vast amounts of CMNV-like particles in the above-mentioned tissues. The differential transcriptome analysis showed that the CMNV infection resulted in the significant down-regulated expression of genes of photo-transduction, digestion, absorption, and growth hormones, which might be the reason for the slow growth of shrimp infected by CMNV. This study uncovered unique characteristics of neurotropism of CMNV for the first time and explored the pathogenesis of slow growth and shell softening of P. vannamei caused by CMNV infection.

show abstract

“…Several studies have developed real-time PCR assays with an LOD of 4 to 10 genome (or plasmid) copies (Table 3). [109][110][111][112] However, it appears that these primers only bind to the genomes of isolates derived from specific geographical regions, as listed in Table 3, due to a significant number of primer mismatches with genomes from other geographical sources (see alignment results for quantitative PCR methods in Figure S2b). For instance, Yan et al 111 developed a TaqMan-based real-time PCR utilising the genomic sequence of an HPV isolate from Korea.…”

Section: Hepatopancreatic Parvovirusmentioning

confidence: 99%

Nucleic acid amplification‐based methods for diagnosis of shrimp viral diseases

Lee,

Vijayan,

Roh

et al. 2023

Reviews in Aquaculture

View full text Add to dashboard Cite

Viral diseases are one of the constraints affecting the sustainability of the shrimp farming industry worldwide. The common causes of viral infections include white spot syndrome virus, infectious hypodermal and haematopoietic necrosis virus, hepatopancreatic parvovirus, Penaeus monodon nudivirus, decapod iridescent virus 1, Taura syndrome virus, yellow head virus, infectious myonecrosis virus and Macrobrachium rosenbergii nodavirus. Accurate diagnostic methods are necessary for preventing the spread of these transboundary diseases. Many molecular diagnostic methods for shrimp viral diseases have been developed and are currently in use, including conventional polymerase chain reaction (PCR), nested PCR, loop‐mediated isothermal amplification PCR, recombinase polymerase assay and real‐time PCR. Although the World Organization for Animal Health (founded as OIE) recommends several PCR methods for shrimp disease diagnosis, there has been a lack of efforts to comparatively evaluate the performance of the available methods, resulting in a significant knowledge gap. Therefore, in this review, we compare various PCR assays and commercial PCR‐based diagnostic kits that have been developed and/or published previously, and discuss pros and cons of each of these methods. Our objective of this review is to offer enhanced clarity and a comprehensive overview of molecular diagnostic methods for shrimp viral diseases. This endeavour would serve as an important resource for researchers engaged in relevant studies and professionals within the shrimp‐farming industry, providing valuable guidance and insights.

show abstract

Detection and quantification of hepatopancreatic parvovirus in penaeid shrimp by real-time PCR assay

Cited by 6 publications

References 21 publications

Development of Real-time PCR Assays for Detection ofDirofilaria immitisfrom Infected Dog Blood

Development of Real-time PCR Assays for Detection ofDirofilaria immitisfrom Infected Dog Blood

Investigation of Pathogenic Mechanism of Covert Mortality Nodavirus Infection in Penaeus vannamei

Nucleic acid amplification‐based methods for diagnosis of shrimp viral diseases

Contact Info

Product

Resources

About