Detection and quantification of Histomonas meleagridis by real-time PCR targeting single copy genes

Hussain, Imtiaz; Jaskulska, Barbara; Heß, Michael; Bilić, Ivana

doi:10.1016/j.vetpar.2015.08.011

Cited by 7 publications

(6 citation statements)

References 23 publications

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“…Designing primers based on the rpb1 gene according to Malik et al . [33], allowed us to create a highly specific and sensitive system: this gene is both discriminatory between different species and genotypes and is highly conserved within the same species [43,44]. All isolated strains of Trichomonas sp .…”

Section: Discussionmentioning

confidence: 99%

Specific clones of Trichomonas tenax are associated with periodontitis

et al. 2019

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Trichomonas tenax, an anaerobic protist difficult to cultivate with an unreliable molecular identification, has been suspected of involvement in periodontitis, a multifactorial inflammatory dental disease affecting the soft tissue and bone of periodontium. A cohort of 106 periodontitis patients classified by stages of severity and 85 healthy adult control patients was constituted. An efficient culture protocol, a new identification tool by real-time qPCR of T. tenax and a Multi-Locus Sequence Typing system (MLST) based on T. tenax NIH4 reference strain were created. Fifty-three strains of Trichomonas sp. were obtained from periodontal samples. 37/106 (34.90%) T. tenax from patients with periodontitis and 16/85 (18.80%°) T. tenax from control patients were detected by culture (p = 0.018). Sixty of the 191 samples were tested positive for T. tenax by qPCR, 24/85 (28%) controls and 36/106 (34%) periodontitis patients (p = 0.089). By combining both results, 45/106 (42.5%) patients were positive by culture and/or PCR, as compared to 24/85 (28.2%) controls (p = 0.042). A link was established between the carriage in patients of Trichomonas tenax and the severity of the disease. Genotyping demonstrates the presence of strain diversity with three major different clusters and a relation between disease strains and the periodontitis severity (p<0.05). More frequently detected in periodontal cases, T. tenax is likely to be related to the onset or/and evolution of periodontal diseases.

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Section: Discussionmentioning

confidence: 99%

Specific clones of Trichomonas tenax are associated with periodontitis

et al. 2019

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“…Research on the bovine parabasalid pathogen Tritrichomonas foetus led to the landmark discoveries of eukaryotic H 2 production in 1957 (370) and the hydrogenosome in 1973 (76). Since then, equivalent organelles have been reported in other pathogenic parabasalids, such as T. vaginalis (16,371), Trichomonas tenax (372), Dientamoeba fragilis (373,374), Pentatrichomonas hominis (375), and Histomonas meleagridis (376)(377)(378). Group A1 [FeFe]-hydrogenases and their maturation factors are localized in these organelles, where they mediate H 2 production (379,380).…”

Section: Eukaryamentioning

confidence: 99%

Molecular Hydrogen Metabolism: a Widespread Trait of Pathogenic Bacteria and Protists

Benoit

Maier

Sawers

et al. 2020

Microbiol Mol Biol Rev

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SUMMARY Pathogenic microorganisms use various mechanisms to conserve energy in host tissues and environmental reservoirs. One widespread but often overlooked means of energy conservation is through the consumption or production of molecular hydrogen (H2). Here, we comprehensively review the distribution, biochemistry, and physiology of H2 metabolism in pathogens. Over 200 pathogens and pathobionts carry genes for hydrogenases, the enzymes responsible for H2 oxidation and/or production. Furthermore, at least 46 of these species have been experimentally shown to consume or produce H2. Several major human pathogens use the large amounts of H2 produced by colonic microbiota as an energy source for aerobic or anaerobic respiration. This process has been shown to be critical for growth and virulence of the gastrointestinal bacteria Salmonella enterica serovar Typhimurium, Campylobacter jejuni, Campylobacter concisus, and Helicobacter pylori (including carcinogenic strains). H2 oxidation is generally a facultative trait controlled by central regulators in response to energy and oxidant availability. Other bacterial and protist pathogens produce H2 as a diffusible end product of fermentation processes. These include facultative anaerobes such as Escherichia coli, S. Typhimurium, and Giardia intestinalis, which persist by fermentation when limited for respiratory electron acceptors, as well as obligate anaerobes, such as Clostridium perfringens, Clostridioides difficile, and Trichomonas vaginalis, that produce large amounts of H2 during growth. Overall, there is a rich literature on hydrogenases in growth, survival, and virulence in some pathogens. However, we lack a detailed understanding of H2 metabolism in most pathogens, especially obligately anaerobic bacteria, as well as a holistic understanding of gastrointestinal H2 transactions overall. Based on these findings, we also evaluate H2 metabolism as a possible target for drug development or other therapies.

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“…Diagnosis of histomonosis was based on postmortem investigations and presence of H meleagridis was confirmed by the PCR protocol according to Hussain et al 25 and was performed on tissue material from caecal lesions.…”

Section: Methodsmentioning

confidence: 99%

“…Coccidiosis was diagnosed based on clinical signs (diarrhoea, increase of mortality), the mucosal gross lesions in the intestine, together with microscopic investigation of scrapings from the intestinal mucosa. Diagnosis of histomonosis was based on postmortem investigations and presence of H meleagridis was conirmed by the PCR protocol according to Hussain et al 25 and was performed on tissue material from caecal lesions.…”

Section: Coccidiosis and Histomonosismentioning

confidence: 99%

Influence of alternative husbandry systems on postmortem findings and prevalence of important bacteria and parasites in layers monitored from end of rearing until slaughter

et al. 2018

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In the present study 66 layer flocks housed in different alternative husbandry systems were monitored from placement of birds on the farm until slaughter to evaluate a possible influence on the occurrence of selected non-infectious as well as infectious diseases. Postmortems were performed and the occurrence of extraintestinal and was investigated. No specific postmortem findings were seen in pullets. Non-infectious diseases were mainly found in layers. Most prominent postmortem findings in layers were reproductive tract lesions and the presence of intestinal helminths. From each flock and from approximately 65 per centof the flocks were isolated. No significant differences were seen in regard to the housing system, but the prevalence of increased with the age of birds. The majority of reproductive tract lesions could be associated with alone or co-infections. The prevalence of and was not influenced by the housing system, but significantly increased with age. Cestodes were present in six flocks. Histomonosis was detected twice. was found in 5 pullet and 20 layer flocks. Additional investigations were performed on demand. Again, reproductive tract lesions were the most prominent postmortem findings. In one flock each histomonosis or erysipelas was diagnosed, respectively. Severe affection by was found once. Necrotic enteritis was seen in two layer flocks.

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Detection and quantification of Histomonas meleagridis by real-time PCR targeting single copy genes

Cited by 7 publications

References 23 publications

Specific clones of Trichomonas tenax are associated with periodontitis

Specific clones of Trichomonas tenax are associated with periodontitis

Molecular Hydrogen Metabolism: a Widespread Trait of Pathogenic Bacteria and Protists

Influence of alternative husbandry systems on postmortem findings and prevalence of important bacteria and parasites in layers monitored from end of rearing until slaughter

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