Detection and quantification of Plasmodium falciparum in human blood by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: a proof of concept study

Stauning, Marius Ahm; Jensen, Christian Salgård; Staalsøe, Trine; Kurtzhals, Jørgen A. L.

doi:10.1186/s12936-023-04719-8

Cited by 4 publications

(4 citation statements)

References 22 publications

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“…Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has brought a revolution in the diagnostic practice for the identification of bacteria and is widely recognized as a method that is fast, and robust, inexpensive, with minimal risk of operator bias. Stauning et al reported a potential application of MALDI-TOD MS providing proof-of-concept for MALDI-TOF MS-based diagnosis of human malaria [118]. The study concluded that MALDI-TOF MS can be applied to the detection and quantification of P. falciparum in human blood albeit not yet applicable to diagnostic practice.…”

Section: Innovative Recently Developed Methodsmentioning

confidence: 99%

The Laboratory Diagnosis of Malaria: A Focus on the Diagnostic Assays in Non-Endemic Areas

Calderaro,

Piccolo,

Chezzi

2024

IJMS

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Even if malaria is rare in Europe, it is a medical emergency and programs for its control should ensure both an early diagnosis and a prompt treatment within 24–48 h from the onset of the symptoms. The increasing number of imported malaria cases as well as the risk of the reintroduction of autochthonous cases encouraged laboratories in non-endemic countries to adopt diagnostic methods/algorithms. Microscopy remains the gold standard, but with limitations. Rapid diagnostic tests have greatly expanded the ability to diagnose malaria for rapid results due to simplicity and low cost, but they lack sensitivity and specificity. PCR-based assays provide more relevant information but need well-trained technicians. As reported in the World Health Organization Global Technical Strategy for Malaria 2016–2030, the development of point-of-care testing is important for the improvement of diagnosis with beneficial consequences for prompt/accurate treatment and for preventing the spread of the disease. Despite their limitations, diagnostic methods contribute to the decline of malaria mortality. Recently, evidence suggested that artificial intelligence could be utilized for assisting pathologists in malaria diagnosis.

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Section: Innovative Recently Developed Methodsmentioning

confidence: 99%

The Laboratory Diagnosis of Malaria: A Focus on the Diagnostic Assays in Non-Endemic Areas

Calderaro,

Piccolo,

Chezzi

2024

IJMS

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“…Matrix-assisted laser desorption/ionization -Time of Flight Mass Spectrometry (MALDI-TOF MS) has brought a revolution in the diagnostic practice for the identification of bacteria and is widely recognized as a method of analysis fast, and robust, inexpensive, with minimal risk of operator bias. Stauning et al reported a potential application of MALDI-TOD MS providing proof-of-concept for MALDI-TOF MS-based diagnosis of human malaria [118]. The study concluded that MALDI-TOF MS can be applied to the detection and quantification of P. falciparum in human blood albeit not yet applicable to diagnostic practice.…”

Section: Innovative Recently Developed Methodsmentioning

confidence: 99%

The Laboratory Diagnosis of Malaria: A Focus on the Diagnostic Assays in Non‐Endemic Areas

Calderaro,

Piccolo,

Chezzi

2023

Preprint

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Malaria is rare in Europe, but it is a medical emergency and programs for its control should ensure access to early diagnosis and prompt treatment within 24-48 h of the symptom’s onset. The consistent number of imported malaria cases and the risk of reappearance of autochthonous cases stimulated laboratories in non-endemic countries to evaluate diagnostic methods/algorithms: microscopy remains the gold standard, but with limitations. RDTs have greatly expanded the ability to diagnose malaria for rapid results due to simplicity and low cost, but they lack sensitivity and specificity. PCR-based assays provide more relevant information but need well-trained technicians. According to WHO global Technical Strategy for Malaria 2016-2030, the future direction for a rapid diagnosis is the development of Point-Of-Care Testing, which would be helpful for prompt/accurate treatment and for preventing the diseases spread. Despite the limitations of current diagnostic methods, they play important roles in dealing with the global malaria situation, including decreasing its incidence. Recently evidence suggested that Artificial Intelligence could be utilized for assisting pathologists in malaria diagnosis. Future studies need to be conducted to see whether this system can be used for routine malaria diagnostic procedures.

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“…MALDI-TOF MS was reported as a method to potentially detect and quantify Plasmodium falciparum in human blood in a recent published study [ 13 ]. It provided a proof-of-concept for the MALDI-TOF-based diagnosis of human malaria; however, while it represents an explorative attempt to use MALDI-TOF MS as a diagnostic tool for malaria, it is far from a practical application.…”

Section: Innovative Applications For Parasite and Virus Identificationmentioning

confidence: 99%

“…Despite the fact that several studies have been reported in the last few years on the application of MALDI-TOF MS to the identification of intestinal protozoa [ 10 , 11 ], helminths [ 12 ], Plasmodium falciparum [ 13 ] or ectoparasites [ 12 ], and Trichomonas vaginalis [ 14 ], MALDI-TOF MS has not yet been used for the routine identification of parasites. Innovative applications of MALDI-TOF MS to virus identification were also reported, with promising results [ 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

MALDI-TOF MS: A Reliable Tool in the Real Life of the Clinical Microbiology Laboratory

Calderaro,

Chezzi

2024

Microorganisms

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Matrix-Assisted Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) in the last decade has revealed itself as a valid support in the workflow in the clinical microbiology laboratory for the identification of bacteria and fungi, demonstrating high reliability and effectiveness in this application. Its use has reduced, by 24 h, the time to obtain a microbiological diagnosis compared to conventional biochemical automatic systems. MALDI-TOF MS application to the detection of pathogens directly in clinical samples was proposed but requires a deeper investigation, whereas its application to positive blood cultures for the identification of microorganisms and the detection of antimicrobial resistance are now the most useful applications. Thanks to its rapidity, accuracy, and low price in reagents and consumables, MALDI-TOF MS has also been applied to different fields of clinical microbiology, such as the detection of antibiotic susceptibility/resistance biomarkers, the identification of aminoacidic sequences and the chemical structure of protein terminal groups, and as an emerging method in microbial typing. Some of these applications are waiting for an extensive evaluation before confirming a transfer to the routine. MALDI-TOF MS has not yet been used for the routine identification of parasites; nevertheless, studies have been reported in the last few years on its use in the identification of intestinal protozoa, Plasmodium falciparum, or ectoparasites. Innovative applications of MALDI-TOF MS to viruses’ identification were also reported, seeking further studies before adapting this tool to the virus’s diagnostic. This mini-review is focused on the MALDI-TOF MS application in the real life of the diagnostic microbiology laboratory.

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Detection and quantification of Plasmodium falciparum in human blood by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: a proof of concept study

Cited by 4 publications

References 22 publications

The Laboratory Diagnosis of Malaria: A Focus on the Diagnostic Assays in Non-Endemic Areas

The Laboratory Diagnosis of Malaria: A Focus on the Diagnostic Assays in Non-Endemic Areas

The Laboratory Diagnosis of Malaria: A Focus on the Diagnostic Assays in Non‐Endemic Areas

MALDI-TOF MS: A Reliable Tool in the Real Life of the Clinical Microbiology Laboratory

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