2002
DOI: 10.1039/b201979p
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Detection and quantification of selenium in proteins by means of gel electrophoresis and electrothermal vaporization ICP-MS

Abstract: A method for the separation of selenium-containing proteins and subsequent detection and quantification of selenium was developed. First, the proteins are fractionated by means of sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE); subsequently, the bands of the gel, containing the proteins, are analyzed by electrothermal vaporization (ETV)-ICP-MS to detect and quantify selenium. External standardization with the use of an internal reference (Te) was applied. A detection limit of ca. 40 pg Se … Show more

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Cited by 42 publications
(21 citation statements)
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“…Therefore some form of post-separation treatment of the gel is required. In most published studies it is mentioned that the gel was dried before ablation, although no details are given [6,24,25,34].…”
Section: Preparation Of Gel For Lamentioning
confidence: 99%
See 1 more Smart Citation
“…Therefore some form of post-separation treatment of the gel is required. In most published studies it is mentioned that the gel was dried before ablation, although no details are given [6,24,25,34].…”
Section: Preparation Of Gel For Lamentioning
confidence: 99%
“…The off-line approaches commonly use either LC methods with fraction collection and element detection in the fractions or combinations of gel-electrophoretic separations with element detection, either following acid-digestion of parts of the gel [6] or by laser ablation (LA)-ICP-MS [7]. The combination of GE techniques and LA-ICP-MS has been successfully used by some groups for the detection and/or identification of heteroelements and metals bound to proteins, after high-resolution separation of the sample.…”
Section: Introductionmentioning
confidence: 99%
“…Size-exclusion liquid chromatography is the fractionation method mostly used in combination with nonradioactive detection methods like ICP-MS, but with the limitation of the low chromatographic resolution typical for size-exclusion [9]. In a previous paper, we reported the use of gel electrophoresis for the fractionation of proteins in combination with electrothermal vaporization-ICP-MS for the detection of Se, with the advantages of a higher resolution than obtainable with size-exclusion and the quantitative aspect of the method [10]. Such an analysis is feasible for a 1-D gel, but is too time-consuming for the analysis of a 2-D gel.…”
Section: Introductionmentioning
confidence: 99%
“…Gel matrix components were separated from analytes of interest via a multistage temperature program prior to high-temperature atomisation/vaporization of Se. Chery et al [53] adopted the ETV approach to quantify Se in selenoprotein bands separated by SDS-PAGE. The gel was silverstained and left to dry.…”
Section: Icp-msmentioning
confidence: 99%