2004
DOI: 10.1016/j.jviromet.2004.01.025
|View full text |Cite
|
Sign up to set email alerts
|

Detection and quantitation of hepatitis E virus in human faeces by real-time quantitative PCR

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2

Citation Types

0
26
0

Year Published

2006
2006
2019
2019

Publication Types

Select...
6
2

Relationship

0
8

Authors

Journals

citations
Cited by 47 publications
(26 citation statements)
references
References 25 publications
0
26
0
Order By: Relevance
“…Several protocols for real-time PCR targeting the ORF3 region (13,17,40) or the ORF2 region (1,16,34,47) That study demonstrated that real-time RT-PCRs are more sensitive than nested PCRs, but the sensitivities of the majority of the assays differed enormously (100-fold to 1,000-fold), independent of the virus strains (3). Among protocols targeting ORF3, we selected one developed by Jothikumar et al, because it was the most frequently used protocol in a recent evaluation performed by Baylis et al (17).…”
Section: Discussionmentioning
confidence: 99%
“…Several protocols for real-time PCR targeting the ORF3 region (13,17,40) or the ORF2 region (1,16,34,47) That study demonstrated that real-time RT-PCRs are more sensitive than nested PCRs, but the sensitivities of the majority of the assays differed enormously (100-fold to 1,000-fold), independent of the virus strains (3). Among protocols targeting ORF3, we selected one developed by Jothikumar et al, because it was the most frequently used protocol in a recent evaluation performed by Baylis et al (17).…”
Section: Discussionmentioning
confidence: 99%
“…The method reported here detected approximately 3.6 ϫ 10 3 copies of in vitro-transcribed HEV RNA. The published real-time PCRs for human and swine HEV (1,8,10,19,20,26,33) have a lower detection limit, but they use DNA as a standard, except for one assay that also used in vitro-transcribed RNA (1). This study used a different method for calculating the standard (number of copies of RNA) compared to the quantitative real-time RT-PCR presented here.…”
Section: Discussionmentioning
confidence: 99%
“…On the basis of this ELISA, several studies about the seroprevalence of avian HEV were carried out (15,28,30). A few RT-PCRs for avian HEV are described (15,27,30,31), but real-time RT-PCR (SYBR green or TaqMan) methods exist only for human and swine HEV (1,8,10,19,20,26). Not all of them are quantitative, and only one (1) uses in vitro-transcribed RNA as a standard for quantification.…”
mentioning
confidence: 99%
“…Both viruses cause acute, self-limited infections that may vary in severity from asymptomatic to fulminant (Purcell & Emerson 2008). The most common route of transmission is generally faecal-oral (Orrù et al 2004) and it causes largescale epidemics.…”
Section: Introductionmentioning
confidence: 99%