Detection and quantitation of human papillomavirus type 16, 18 and 52 DNA in the peripheral blood of cervical cancer patients

Ho, Chih Ming; Yang, Shwu Huey; Chien, Tsai Yen; Huang, Shih‐Han; Jeng, Cherng Jye; Chang, Shwu Fen

doi:10.1016/j.ygyno.2005.07.004

Cited by 47 publications

(44 citation statements)

References 20 publications

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“…Although the exact mechanism via which viral DNA is released into the bloodstream remains unclear, several pathways have been proposed, including necrosis and apoptosis [35]. …”

Section: Discussionmentioning

confidence: 99%

5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)

Cortés‐Gutiérrez

Ortíz-Hernández

Dávila-Rodríguez

et al. 2013

IJMS

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We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH). A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with low-grade squamous intraepithelial lesions (LG-SIL), 10 with high-grade SIL (HG-SIL), and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1.

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“…Although the exact mechanism via which viral DNA is released into the bloodstream remains unclear, several pathways have been proposed, including necrosis and apoptosis [35]. …”

Section: Discussionmentioning

confidence: 99%

5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)

Cortés‐Gutiérrez

Ortíz-Hernández

Dávila-Rodríguez

et al. 2013

IJMS

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“…This has long been debated [28]. The role of viremia in the pathogenesis of HPV-related diseases is still unclear, although HPV DNA has been detected in peripheral blood in some studies, though in varying amounts [29-32]. …”

Section: Discussionmentioning

confidence: 99%

Association between human papillomavirus DNA and temporal arteritis

Mohammadi

Pfeifer

Lewis

2012

BMC Musculoskelet Disord

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“…Previous PCR-based studies detecting HPV DNA in blood used different material (serum, plasma or blood), DNA extraction methods, and primers for amplifying HPV DNA 1–8,11. Some of these studies also included patients with pre-invasive cervical disease, but the findings were inconsistent.…”

Section: Discussionmentioning

confidence: 99%

“…Yang et al detected HPV 16 DNA in the serum and peripheral blood fraction of four out of five patients with untreated high-grade cervical dysplasia, but not in any of the individuals who had been treated and had no active lesions at the time of study 1. Ho et al failed to detect HPV 16, HPV 18, or HPV 52 DNA in the peripheral blood of 10 CIN I patients, 10 CIN II patients, and 20 CIN III patients 8. Kay et al detected HPV 16 and HPV 18 DNA in the blood of 24.4% of patients with advanced cervical cancer, but not in any of the patients with cervical HPV infection or pre-invasive cervical lesions 11.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have demonstrated HPV DNA detection using PCR-based methods in serum,1–3 plasma,4–7 and peripheral blood1,8 of patients with invasive cervical cancer, suggesting potential diagnostic and prognostic applications. If circulating HPV DNA was reliably detected in women with pre-invasive cervical disease, the method could have promise for cervical cancer screening.…”

Section: Introductionmentioning

confidence: 99%

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