Detection and Spectrophotometric Determination of Pyruvic Acid

Mehrotra, Raj N.; Hasan, Tausiful

doi:10.1080/00032718608066497

Cited by 2 publications

(1 citation statement)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The following supporting information can be downloaded at: https:// www.mdpi.com/article/10.3390/pr10112186/s1, Figure S1: Structure of [Fe 3 O(CH 3 COCO 2 ) 6 (H 2 O) 3 ] + [23]; Figure S2: (A) Four S. cerevisiae strains, GJ2009, GJ2010, GJ2011, and GJ2012 (derived from GJ2008) with different ethanol synthesis capacities, were verified by fermentation under 190 g/L sucrose. (B) 120 µL of these four S. cerevisiae strains' fermentation supernatant (Five times dilution) was transferred to a 96-well enzyme label plate, and 80 µL 1M Fe(NO 3 ) 3 was added for reaction at room temperature for 10 min.…”

Section: Supplementary Materialsmentioning

confidence: 99%

A High-Throughput Screening Procedure (Py-Fe3+) for Enhancing Ethanol Production by Saccharomyces cerevisiae Using ARTP Random Mutagenesis

Wang

Xie

et al. 2022

Processes

View full text Add to dashboard Cite

Saccharomyces cerevisiae is an important microbial organization involved in ethanol synthesis. Mutant strains that can withstand multiple pressures during this process are critical to the industrial development of biofuels. In this study, a dual high-throughput screening method of Triphenyl-2H-tetrazoliumchloride (TTC)-based macroscopic observation and the reaction of ferric nitrate with pyruvate (or pyruvate radical ion) in fermentation broth was used. Using this, an S. cerevisiae mutant library that could tolerate 381 g/L sucrose was established by ARTP random mutation and adaptive evolution to select the best strain; its ethanol yield was increased by an additional 20.48%; and the sucrose utilization rate was 81.64%. This method is specific to the selection of strains with increased ethanol production.

show abstract

Section: Supplementary Materialsmentioning

confidence: 99%

A High-Throughput Screening Procedure (Py-Fe3+) for Enhancing Ethanol Production by Saccharomyces cerevisiae Using ARTP Random Mutagenesis

Wang

Xie

et al. 2022

Processes

View full text Add to dashboard Cite

show abstract

Activity of the liver mitochondrial aspartate aminotransferase and malate dehydrogenase in rats with toxic hepatitis under conditions of alimentary protein deficiency

Voloshchuk¹,

Kopylchuk²

2019

Bìol. Tvarin

View full text Add to dashboard Cite

Nutritional demands in proteins depend on the life stage and health status of organisms. Both humans and experimental animals under stress conditions and especially drug processing become to be more sensitive to the protein deficit in the food. In this study, we examined some acetaminophen-induced metabolic effects potentiated by alimentary protein deprivation (APD) in rat liver. In particular, activities of the liver mitochondrial aspartate aminotransferase and malate dehydrogenase in rat liver were studied in conditions of balanced and imbalanced by protein diets of isocaloric content. It has been found that acute acetaminophen-induced hepatitis in comparison to control does not change the activity of mitochondrial malate dehydrogenase causing simultaneous 4-fold reduction in activity of mitochondrial aspartate aminotransferase and 2.5-fold reduction of mitochondrial oxaloacetate content. Interestingly, alimentary protein deprivation enhances the effects of acetaminophen on the described parameters. Finally, in order to confirm these associations between amount of the protein in the rat diet and physiological measures in their liver with toxic injury, principal component analysis (PCA) was performed. Two principal components characterize changes in physiological measures in our study. Principal component 1 explains about 86 % of the variation among whole dataset mainly related to control group and group subjected to acetaminophen treatment with simultaneous APD. It reveals the tight association of scores for AST activity and oxaloacetate level with control group, which might indicate the high efficiency in the oxaloacetate conversion by AST lacked in both groups with hepatitis. Similarly, principal component 1 explaining the variance in MDH activity shows its linkage to the control group, indicating the importance of MDH for the health status of control animals. On the other side, principal component 2 reveals close association between lactate and pyruvate levels as well as cytosolic NAD + /NADH ratio with acetaminophen-treated group of animals subjected to APD, confirming that toxic liver injury associated with low protein consumption leads to increased lactate-pyruvate turnover in cytosol affecting. This potentially might be associated with energy-generating dysfunction in liver under toxic hepatitis on the background of dietary protein deficiency.

show abstract

Detection and Spectrophotometric Determination of Pyruvic Acid

Cited by 2 publications

References 13 publications

A High-Throughput Screening Procedure (Py-Fe3+) for Enhancing Ethanol Production by Saccharomyces cerevisiae Using ARTP Random Mutagenesis

A High-Throughput Screening Procedure (Py-Fe3+) for Enhancing Ethanol Production by Saccharomyces cerevisiae Using ARTP Random Mutagenesis

Activity of the liver mitochondrial aspartate aminotransferase and malate dehydrogenase in rats with toxic hepatitis under conditions of alimentary protein deficiency

Contact Info

Product

Resources

About