Detection and Surveillance of Harmful Algal Bloom Species and Toxins

Doucette, Gregory J.; Medlin, Linda K.; McCarron, Pearse; Heß, Philipp

doi:10.1002/9781118994672.ch2

Cited by 13 publications

(12 citation statements)

References 399 publications

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“…Most of the technologies listed in Table 1 are discussed in greater detail in subsequent sections. Readers are directed toward additional reviews which provide a more detailed treatment of many of the technologies that are not discussed in detail (e.g., Ottesen, 2016;Doucette and Kudela, 2017;Glibert et al, 2018) and/or which focus more on classical methods for cell and/or toxin detection (e.g., Frolov et al, 2013;Trainer and Hardy, 2015;Zhang and Zhang, 2015;Otten and Paerl, 2016;Reguera et al, 2016; Association of Public Health Laboratories [APHL], 2017; Medlin and Orozco, 2017;Doucette et al, 2018;Glibert et al, 2018).…”

Section: State Of the Technologymentioning

confidence: 99%

Considerations in Harmful Algal Bloom Research and Monitoring: Perspectives From a Consensus-Building Workshop and Technology Testing

Stauffer

Bowers

Buckley³

et al. 2019

Front. Mar. Sci.

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Recurrent blooms of harmful algae and cyanobacteria (HABs) plague many coastal and inland waters throughout the United States and have significant socioeconomic impacts to the adjacent communities. Notable HAB events in recent years continue to underscore the many remaining gaps in knowledge and increased needs for technological advances leading to early detection. This review summarizes the main research and management priorities that can be addressed through ocean observationbased approaches and technological solutions for harmful algal blooms, provides an update to the state of the technology to detect HAB events based on recent activities of the Alliance for Coastal Technologies (ACT), offers considerations for ensuring data quality, and highlights both ongoing challenges and opportunities for solutions in integrating HAB-focused technologies in research and management. Specifically, technological advances are discussed for remote sensing (both multispectral satellite and hyperspectral); deployable in situ detection of HAB species on fixed or mobile platforms (based on bulk or taxa-specific biomass, images, or molecular approaches); and field-based and/or rapid quantitative detection of HAB toxins (via molecular and analytical chemistry methods). Suggestions for addressing challenges to continued development and adoption of new technologies are summarized, based on a consensus-building workshop hosted by ACT, including dealing with the uncertainties in investment for HAB research, monitoring, and management. Challenges associated with choosing appropriate technologies for a given ecosystem and/or management concern are also addressed, and examples of programs that are leveraging and combining complementary approaches are highlighted.

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Section: State Of the Technologymentioning

confidence: 99%

Considerations in Harmful Algal Bloom Research and Monitoring: Perspectives From a Consensus-Building Workshop and Technology Testing

Stauffer

Bowers

Buckley³

et al. 2019

Front. Mar. Sci.

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“…McPartlin et al, 2017), imaging flow cytometry (IFC) (Buskey and Hyatt, 2006;Campbell et al, 2010Campbell et al, , 2013, and satellite analysisbased algorithms. The FlowCAM (Yokogawa Fluid Imaging Technologies, United States) is an imaging flow cytometer designed to characterize microplankton size range particles (Sieracki et al, 1998) and used by different research groups for semi-automated monitoring of algal blooms (Buskey and Hyatt, 2006;Lehman et al, 2013;Dashkova et al, 2017). FlowCAM software have been allowed to discriminate and quantitate different potentially toxic algae within complex natural assemblages, including Microcystis colonies (Buskey and Hyatt, 2006;Lehman et al, 2013Lehman et al, , 2017Sukenik et al, 2015;Graham et al, 2018;Kurobe et al, 2018;Mirasbekov et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Combining Imaging Flow Cytometry and Molecular Biological Methods to Reveal Presence of Potentially Toxic Algae at the Ural River in Kazakhstan

et al. 2021

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Algal blooms occur in freshwater bodies throughout the world, often leading to fish kills. Cases of these kills along the Ural River were reported in 2018–2019, involving significant amount of sturgeon in fish farming areas. In this study, the analysis of algal samples from the delta of the Ural River up to 100 km inland was carried out from August to December 2019 using imaging flow cytometry (IFC), molecular biological, and microscopic techniques. We identified the filamentous cyanobacteria Cuspidothrix issatschenkoi, Dolichospermum cf. flos-aquae, Dolichospermum cf. macrosporum, Pseudanabaena limnetica, and Planktothrix spp. as the dominant potentially toxic phytoplankton species, and we also found minor quantities of Cylindrospermopsis raciborskii. For the first time, molecular phylogenetic investigations of field clones of cyanobacteria from Ural River were carried out to establish the taxa of the dominant species and to identify the presence of genes encoding toxins. The complementary analysis with nanopore-based next-generation sequencing overlapped with the results of IFC and was instrumental in revealing minor cyanobacteria taxa. Real-time PCR analysis and sequencing indicated the presence of Microcystis and ADA-clade spp. as well as genes associated with the production of microcystin (mcyE) and the algal neurotoxin saxitoxin (sxtA) originating from cyanobacteria. These findings suggest that toxin-producing cyanobacteria could become a threat in the Ural River near Atyrau, which can significantly affect aquaculture in the region.

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“…Since a bloom population can range from minimally toxic (or even non-toxic) to highly toxic (even over the course of a single event) depending on environmental factors as well as the presence and/or absence of toxigenic strains, determination of both cells and toxins provides the most complete and useful dataset to inform early warning systems. Similarly, toxin accumulation in vector species can be decoupled from the aforementioned measurements, so it is often important to measure toxin levels in vector species (for example, Abraham and Rambla-Alegre, 2017; A wide range of well-established, conventional methods as well as more advanced, and in some cases autonomous, technologies, have been used to monitor levels of HAB cells and toxins (Zhang and Zhang, 2015;Doucette et al, 2018). The latter represents a rapidly emerging area for development of HAB detection and surveillance tools, which has been the subject of several recent reviews (for example, Vilariño et al, 2013;Doucette and Kudela, 2017;Gilbert et al, 2018).…”

Section: Field Observation Methodsmentioning

confidence: 99%

Joint FAO-IOC-IAEA technical guidance for the implementation of early warning systems for harmful algal blooms

2023

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The designations employed and the presentation of material in this information product do not imply the expression of any opinion whatsoever on the part of the Food and Agriculture Organization of the United Nations (FAO) concerning the legal or development status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. The mention of specific companies or roducts of manufacturers, whether or not these have been patented, does not imply that these have been endorsed or recommended by FAO in preference to others of a similar nature that are not mentioned.The views expressed in this information product are those of the author(s) and do not necessarily reflect the views or policies of FAO.

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Detection and Surveillance of Harmful Algal Bloom Species and Toxins

Cited by 13 publications

References 399 publications

Considerations in Harmful Algal Bloom Research and Monitoring: Perspectives From a Consensus-Building Workshop and Technology Testing

Considerations in Harmful Algal Bloom Research and Monitoring: Perspectives From a Consensus-Building Workshop and Technology Testing

Combining Imaging Flow Cytometry and Molecular Biological Methods to Reveal Presence of Potentially Toxic Algae at the Ural River in Kazakhstan

Joint FAO-IOC-IAEA technical guidance for the implementation of early warning systems for harmful algal blooms

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