Detection and Survival of Vibrio Species in Shrimp (Penaeus indicus) and Mussel (Mytilus galloprovincialis) at Landing and after Processing at Seafood Markets in Suez, Egypt

Ahmed, Omaima; Amin, Hesham F.

doi:10.21608/jfds.2018.36107

“…After an additional step of purification with CENTRI-SEP Columns (Princeton Separations, Freehold, NJ), DNA sequencing was carried out by 3500 Genetic Analyzer (Applied Biosystems, Massachusetts, USA). The obtained consensus sequences were subjected to BLAST search through the Mega program (7.0.20) [27,28].…”

Section: Dna Sequencingmentioning

confidence: 99%

Bacteria Associated with Fresh-water Aquaculture Tilapia Fish (Oreochromisniloticus) in Suez, Egypt

Ahmed¹

2019

Food Sci Nutr Res

2

0

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Nile tilapia (Oreochromis niloticus) production is ranked on the top of aquaculture fish in Egypt. This study was aimed to screen bacteria associated with aquaculture freshwater tilapia to determine the microbiological safety of these wide distributed fish in Egypt. The mean viable bacterial count from fish fillets with the skin samples revealed 5.6 ± 0.8 logs CFU/g. A total of eleven (11) bacterial species were isolated and identified including: E. coli, E. coli O157: H7, Salmonella enterica, Morganella morganii, Proteus mirabilis, Proteus vulgaris, Enterobacter cloacae, Enterobacter cancerogenus, Hafnia alvei, Aeromonas hydrophila, Photobacterium damaselae. The frequency of occurrences of the isolated bacteria indicated that Enterobacter cloacae had the highest frequency of occurrence (12%), while one isolate (2%) of Salmonella enterica, E coli O157:H7, and Aeromonas hydrophila were detected. These bacterial species are potentially pathogenic to humans. Therefore, hygienic handling methods and proper processing are needed before consumption of this fish.

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“…They are also a portion of the natural microflora of crustacea, fish, and shellfish [10]. Some species of vibrios including V. vulnificus and V. parahaemolyticus are pathogenic and may cause seafoodborne illnesses in humans [11][12][13]. Seafood-borne outbreaks caused by vibrios in humans have been reported worldwide [14,15].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Performance, Microbial Community, Bacterial Food Quality, and Gene Expression of Whiteleg Shrimp (Litopenaeus vannamei) Reared under Different Density Biofloc Systems

Said

¹

,

El-barbary

²

,

Ahmed

³

2022

Aquaculture Nutrition

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Biofloc shrimp culture, as a way of improving shrimp production, gains worldwide consideration. However, the effects of the biofloc system on shrimp culture at high densities could be a challenge. Here, this study is aimed at identifying a better stocking density of whiteleg shrimp (Litopenaeus vannamei) between two intensive biofloc systems of 100 and 300 org./m2. Achieving that was done by comparing growth performance, water quality, feed utilization, microbial loads from water and shrimps, and gene expression of growth, stress, and immune-related genes. Shrimp postlarvae with a mean weight of 35.4 ± 3.7 mg were reared in six indoor cement tanks (36 m3 total capacity each) at two stocking densities (3 replicates each) for a rearing period of 135 days. Better final weight, weight gain, average daily weight gain, specific growth rate, biomass increase percentage, and survival rate were associated with lower density (100/m2), whereas high-density showed significantly higher total biomass. Better feed utilization was found in the lower density treatment. Lower density treatment enhanced water quality parameters, including higher dissolved oxygen and lower nitrogenous wastes. Heterotrophic bacterial count in water samples was recorded as 5.28 ± 0.15 and 5.11 ± 0.28 log CFU/ml from the high- and low-density systems, respectively, with no significant difference. Beneficial bacteria such as Bacillus spp. were identified in water samples from both systems, still, the Vibrio-like count was developed in the higher density system. Regarding shrimp food bacterial quality, the total bacterial count in the shrimp was recorded as 5.09 ± 0.1 log CFU/g in the 300 org./m2 treatment compared to 4.75 ± 0.24 log CFU/g in the lower density. Escherichia coli was isolated from the shrimps in a lower density group while Aeromonas hydrophila and Citrobacter freundii were associated with shrimps from a higher density system. Immune-related genes including prophenoloxidase, superoxide dismutase (SOD), and lysozyme (LYZ) expressions were all significantly higher expressed in the shrimp from the lower density treatment. Toll receptor (LvToll), penaiedin4 (PEN4), and stress-related gene (HSP 70) showed a decreased gene expression in the shrimp raised in the lower density. Significant upregulation of growth-related gene (Ras-related protein-RAP) expression was associated with the lower stocking density system. In conclusion, the current study found that applying high stocking density (300 org./m2) contributes negatively to performance, water quality, microbial community, bacterial food quality, and gene expression of immune, stress, and growth-related genes when compared with the lower stocking density system (100 org./m2) under biofloc system.

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“…They are also considered part of the natural microflora of fish and shellfish [24]. Other species of vibrios such as V. parahaemolyticus and V. vulnificus are pathogenic for humans that cause seafood-borne illnesses [25][26][27]. Seafood foodborne outbreaks in humans have been reported worldwide [28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Carbohydrate Supplement Impact on Growth Performance, Bacterial Community, and Bacterial Food Quality of Whiteleg Shrimp (Litopenaeus vannamei) under Biofloc System

Said

¹

,

Ahmed

²

2022

Aquaculture Nutrition

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Biofloc technology has a high impact on enhancing shrimp production. Suitable supplemented carbohydrates (CHO) could affect the type of microorganisms developed in the system, which reflects on shrimp production, food safety, and public health. Here, we aimed to compare the effects of sugarcane molasses and wheat flour as carbohydrate sources on biofloc technology. That was achieved through measuring the following parameters: water quality, growth performance, feed utilization, floc composition, shrimp whole body composition, microbial community, and biofloc shrimp food bacterial quality. Postlarvae of whiteleg shrimp (Litopenaeus vannamei) with a mean weight ( 38.47 ± 5.8 mg ) were stocked at a density of 200 individuals/m2 and cultured under a biofloc system for 128 days in six tanks with a total water volume of 30 m2 each. Water quality analysis revealed a better-dissolved oxygen concentration (5.59 mg/L) in the wheat flour treatment, whereas no significant differences were found between the two treatments in ammonia, nitrite, and pH levels. Increased turbidity (64.27 NTU) and floc volume (18.40 mL/L) were recorded with molasses treatment. Growth performance including final weight, weight gain, average daily gain, weight gain per week, and specific growth rate (12.37 g, 12.34 g, 0.096 g/d, 0.68, and 4.70%, respectively) were all significantly higher in the molasses treatment. Wheat flour treatment was associated with a higher survival rate (99%), biomass (71.16 Kg), and biomass increase percentage (395.337) in shrimps. It also improved feed utilization in terms of a lower feed conversion ratio (1.37) and higher protein efficiency ratio (1.92). The chemical composition of biofloc and shrimp whole body were both nutritious higher in wheat flour treatment. In water, total heterotrophic bacterial counts with sugarcane molasses treatment and wheat flour were estimated as 3.4 × 10 5 CFU / mL and 1.2 × 10 5 CFU / mL , respectively, with no significant difference. In both treatments, beneficial bacteria such as lactic acid bacteria and Enterobacter cloacae were identified in water with the absence of pathogenic Vibrio spp. Wheat flour had a significantly lower total Vibrio-like count (TVC). Shrimps had lower TVC ( 1.9 × 10 4 CFU / g ) with flour than with molasses ( 1.32 × 10 5 CFU / g ). Cronobacter spp. were associated with shrimps in BFT supplemented with molasses, which might pose a potential risk to food safety. In conclusion, the use of wheat flour was the best for shrimp production and shrimp food bacterial quality.

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Detection and Survival of Vibrio Species in Shrimp (Penaeus indicus) and Mussel (Mytilus galloprovincialis) at Landing and after Processing at Seafood Markets in Suez, Egypt

Cited by 3 publications

References 26 publications

Bacteria Associated with Fresh-water Aquaculture Tilapia Fish (Oreochromisniloticus) in Suez, Egypt

Bacteria Associated with Fresh-water Aquaculture Tilapia Fish (Oreochromisniloticus) in Suez, Egypt

Assessment of Performance, Microbial Community, Bacterial Food Quality, and Gene Expression of Whiteleg Shrimp (Litopenaeus vannamei) Reared under Different Density Biofloc Systems

Carbohydrate Supplement Impact on Growth Performance, Bacterial Community, and Bacterial Food Quality of Whiteleg Shrimp (Litopenaeus vannamei) under Biofloc System

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