Detection and Titration of Staphylococcus aureus Agglutinin in Serum.

Boger, William P.; Frankel, Jack W.; Gavin, John J.

doi:10.3181/00379727-104-25935

Cited by 10 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No P. mirabilis agglutinins were detected (<1:2) in the serum of nonimmunized rabbits. Agglutinins against S. aureus were tested by the method of Boger et al (2); the reciprocal of the agglutinin titer was 1:12,800. Before use, all sera were heat inactivated for 30 min at 56°C.…”

mentioning

confidence: 99%

Factors influencing the immune enhancement of intrapulmonary bactericidal mechanisms

Jakab¹

1976

Infect Immun

View full text Add to dashboard Cite

The effect of specific immunization on the antibacterial defense mechanisms of the murine lung was assessed against Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus aureus (Smith), Serratia marcescens, Klebsiella pneumoniae, Proteus mirabilis, and Pseudomonas aeruginosa. Immunization by aerosol inhalation significantly enhanced the intrapulmonary killing of Pseudomonas aeruginosa and Proteus mirabilis but not the remaining organisms. With P. mirabilis, systemic immunization induced higher titers of specific serum agglutinins as compared with local respiratory tract immunization; however, local immunization was more effective in enhancing pulmonary bactericidal activity than was parenteral vaccination. Passive immunity against P. mirabilis or aerogenic challenge with preopsonized P. mirabilis significantly enhanced intrapulmonary killing of the homologous organism. With S. aureus, pulmonary bactericidal activity was not accelerated by aerosol challenge with the preopsonized organism, nor was it accelerated in passively immunized mice. These data demonstrate that the immune enhancement of pulmonary bactericidal activity is governed by the bacterium used for challenge and the route of immunization. The results further demonstrate that with P. mirabilis, antibody-mediated mechanisms are involved in the immune enhancement of pulmonary bactericidal activity.

show abstract

mentioning

confidence: 99%

Factors influencing the immune enhancement of intrapulmonary bactericidal mechanisms

Jakab¹

1976

Infect Immun

View full text Add to dashboard Cite

show abstract

“…Immunologic Response of Runted Animals.--As might be expected, no circulating antibody could be detected in runted animals during the period of treatment with the bacterial vaccines. These determinations were carried out, using either the capillary precipitin reaction with an ammonium sulfate fraction derived from homologous organisms (Ekstedt, reference 1 c) as antigen, or by a sensitive growth-agglutination test described by Boger, Frankel, and Gavin (5). In the latter test the compact variant of the Smith strain of S aureus was used as the antigen.…”

Section: Cmentioning

confidence: 99%

Runt Disease Induced in Neonatal Mice by Sterile Bacterial Vaccines

Ekstedt

Nishimura

1964

The Journal of Experimental Medicine

View full text Add to dashboard Cite

A form of runt disease has been produced in neonatal CF-1 and ICR mice by the repeated injection of 109 washed, autoclaved, saline-suspended staphylococci or streptococci. The most severely affected animals showed a marked decrease in lymphoid tissues and resembled grossly and microscopically animals suffering from the classical runt or wasting disease described by others. The timing of the initial antigenic stimulation was of importance, and animals started on their course of injections at an age of 48 hours or older showed no effect. There was a considerable variation in the severity of the disease within litters and from one litter to another. This variation could not be ascribed to a difference in susceptibility between sexes, since both male and female mice were observed to runt under appropriate conditions. Germ-free ICR mice were much more resistant to the runting phenomenon than conventional animals of the same strain, but could be induced to runt by injection of the staphylococcal vaccine suspended in homologous antiserum. The relationship of the runting phenomenon described here and classical runt disease or runting by adrenal hormones is discussed.

show abstract

“…Maximal inhibitory dilutions (MID) of the plasma were determined against a penicillin sensitive strain of Staphylococcus aureus ATCC 6538 P and a penicillin resistant strain, Standard # 7 of Boger et al (1961). This strain has been defined through the courtesy of Dr.…”

Section: Aterial and Methodsmentioning

confidence: 99%

“…Two-fold serial dilutions of each plasma sample were made in Trypticase-Soy Broth (Baltimore Biological Laboratories) in the manner described previously (Boger, et al, 1961). Each dilution was inoculated with 106 viable organisms as determined by plate count.…”

Section: Aterial and Methodsmentioning

confidence: 99%