Detection and typing of human-infecting influenza viruses in China by using a multiplex DNA biochip assay

Wang, Yongqiang; Qu, Jiuxin; Ba, Qi; Dong, Jiuhong; Zhang, Liang; Zhao, Hong; Wu, Aiping; Wang, Dayang; Xia, Zanxian; Peng, Daxin; Shu, Yuelong; Cao, Bin; Jiang, Taijiao

doi:10.1016/j.jviromet.2016.04.021

Cited by 8 publications

(8 citation statements)

References 33 publications

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“…In our research, multiple amplification techniques were combined with membrane biochips to simultaneously detect nine common sepsis pathogens in a single reaction. The sensitivity of the assay varied in genomic DNA of septicemic pathogens and simulated clinical samples, and the detection range was 5–100 copies/reaction and 20–200 cfu/reaction, respectively, which showed similar phenomena with the previous studies ( Thanthrige-Don et al, 2018 ; Wang et al, 2016 ). Wang et al (2016) reported that the sensitivity for different viruses varied in the range of 2–100 copies/reaction using the multiplex RT-PCR combined with membrane biochip assay.…”

Section: Discussionsupporting

confidence: 82%

“…The sensitivity of the assay varied in genomic DNA of septicemic pathogens and simulated clinical samples, and the detection range was 5–100 copies/reaction and 20–200 cfu/reaction, respectively, which showed similar phenomena with the previous studies ( Thanthrige-Don et al, 2018 ; Wang et al, 2016 ). Wang et al (2016) reported that the sensitivity for different viruses varied in the range of 2–100 copies/reaction using the multiplex RT-PCR combined with membrane biochip assay. Thanthrige-Don et al (2018) reported that the analytical sensitivities of multiplex PCR could detect 1–10 copies/reaction in 21 gene targets, even with the lowest sensitivity of 1,000 copies/reaction in the BtoV N gene.…”

Section: Discussionsupporting

confidence: 82%

“…The main steps were done using our previous methods ( Wang et al, 2016 ). Membrane biochip hybridization was performed in an automatic membrane-based biochip instrument (MFS-8, DRAGONLAB, Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, a multiplex DNA biochip assay was used to detect and type human-infecting influenza viruses. Eighty-one clinical samples, including 66 positive samples with evident seasonal influenza virus infections, were tested, and the clinical sensitivity and specificity were 95.5% and 100% respectively ( Wang et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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A new application of multiplex PCR combined with membrane biochip assay for rapid detection of 9 common pathogens in sepsis

Zhao

Wang

et al. 2023

PeerJ

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Rapid and accurate identification of specific sepsis pathogens is critical for patient treatment and disease control. This study aimed to establish a new application for the rapid identification of common pathogens in patients with suspected sepsis and evaluate its role in clinical application. A multiplex PCR assay was designed to simultaneously amplify specific conserved regions of nine common pathogenic microorganisms in sepsis, including Acinetobacter baumannii, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumonia, and Candida albicans. The PCR products were analyzed by a membrane biochip. The analytical sensitivity of the assay was determined at a range of 5–100 copies/reaction for each standard strain, and the detection range was 20–200 cfu/reaction in a series dilution of simulated clinical samples at different concentrations. Out of the 179 clinical samples, the positive rate for pathogens detected by the membrane biochip assay and blood culture method was 20.11% (36/179) and 18.44% (33/179), respectively. However, by comparing the positive rate of the nine common pathogens we detected, the membrane biochip assay tended to be more sensitive than the blood culture method (20.11% vs 15.64%). The clinical sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the membrane biochip assay were 92.9%, 93.2%, 72.2% and 98.6%, respectively. Generally, this multiplex PCR combined membrane biochip assay can be used to detect major sepsis pathogens, and is useful for early initiation of effective antimicrobial treatment, and is feasible for sepsis pathogens identification in routine clinical practice.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 82%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A new application of multiplex PCR combined with membrane biochip assay for rapid detection of 9 common pathogens in sepsis

Zhao

Wang

et al. 2023

PeerJ

View full text Add to dashboard Cite

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“…Wang et al . [10] found that the application of RT-PCR coupled with membrane-based DNA biochip can detect and discriminate the type (A and B) and the subtype (human H1N1, human H3N2, avian H5N1 and avian H7N9) of influenza viruses in circulation in China. PCR products were analyzed by a membrane-based biochip.…”

Section: Influenza Virus Detection Methodsmentioning

confidence: 99%

Progress and current status of influenza researches in China

Shi

Feng

Zhang

2019

Journal of Translational Internal Medicine

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Influenza pandemics with different extent occur every year in the world. It can cause high morbidity and mortality, arouse fear panic in public, and attract extensive attention worldwide. This paper reviews the research progress in epidemiological characteristics, detection methods, pathogenesis, treatment and prophylactic measures of influenza in China. It will be helpful for us to understand the current situation of influenza.

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Facilely prepared low-density DNA monolayer–based electrochemical biosensor with high detection performance in human serum

Jin-yuan

Liu

et al. 2019

Anal Bioanal Chem

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Detection and typing of human-infecting influenza viruses in China by using a multiplex DNA biochip assay

Cited by 8 publications

References 33 publications

A new application of multiplex PCR combined with membrane biochip assay for rapid detection of 9 common pathogens in sepsis

A new application of multiplex PCR combined with membrane biochip assay for rapid detection of 9 common pathogens in sepsis

Progress and current status of influenza researches in China

Facilely prepared low-density DNA monolayer–based electrochemical biosensor with high detection performance in human serum

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