Detection, characterisation, and quantification of carnosine and other histidyl derivatives in cardiac and skeletal muscle

O'Dowd, John J.; Robins, David J.; Miller, D. J.

doi:10.1016/0304-4165(88)90015-3

Cited by 112 publications

(67 citation statements)

References 31 publications

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“…) N-acetyl L-histidine (Fig. 1), another imidazole found in heart muscle from the frog (Kuroda & Ikoma, 1966) and mammals (Sobue, Konishi & Nakajima, 1975;Crichton, Lamont, Miller & O'Dowd, 1988;O'Dowd, Robins & Miller, 1988), was tested in several cardiac preparations. It has a much lower Ca2+ sensitizing effect, producing no clear alteration in log K2.…”

Section: Methodsmentioning

confidence: 99%

“…New evidence (see O'Dowd et al 1988;House et al 1989 a, b) suggests that the total level of histidyl compounds of this type in mammalian heart may be several millimoles although carnosine and N-acetyl L-histidine only represent a small fraction of the total. Homocarnosine is a significant constituent of rat ventricle and its ability to Ca2+-sensitize and promote maximum Ca2+-activated force, together with the Ca2+ sensitizing effect of anserine, have been described here.…”

Section: Physiological and Pathological Implicationsmentioning

confidence: 99%

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Calcium sensitizing action of carnosine and other endogenous imidazoles in chemically skinned striated muscle.

Lamont

Miller

1992

The Journal of Physiology

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SUMMARY1. The imidazole-containing compounds carnosine and homocarnosine, endogenous to skeletal and cardiac muscle, have been tested for effect on the contractile behaviour of chemically skinned (saponin or Triton X-100) skeletal and cardiac muscle.2. Carnosine, at millimolar concentrations which are near physiological for many skeletal fibres, and in a concentration-dependent fashion, shifts the curve relating[Ca21] to steady-state tension to lower [Ca2"] in both skeletal (frog but not crab) and cardiac (rat) muscle preparations.3. Of other imidazoles endogenous to heart, homocarnosine is somewhat more effective, while N-acetyl L-histidine is much less so.4. The maximum level of Ca2+-activated force is increased significantly by homocarnosine in cardiac trabeculae.5. We propose that the cellular imidazoles related to carnosine are natural 'Ca21 sensitizers' in striated muscle. Alterations in their levels as a result of disease or training, and between different fibre types. may contribute to differences in contractile performance of the intact tissues.

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Section: Methodsmentioning

confidence: 99%

Section: Physiological and Pathological Implicationsmentioning

confidence: 99%

Calcium sensitizing action of carnosine and other endogenous imidazoles in chemically skinned striated muscle.

Lamont

Miller

1992

The Journal of Physiology

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“…Carnosine and its related compounds, homocarnosine and anserine, are present at relatively high concentrations (1-20 mM) in the muscle and brain tissues of humans and other vertebrates (26,27). These compounds have been shown to be extremely efficient antioxidant molecules (28).…”

Section: Effects Of Carnosine and Related Compounds On The Ferritin/ mentioning

confidence: 99%

Reaction of ferritin with hydrogen peroxide induces lipid peroxidation

Yoon¹,

Lee²,

Kang³

2010

BMB Reports

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Lipid peroxidation is known to be an important factor in the pathologies of many diseases associated with oxidative stress. We assessed the lipid peroxidation induced by the reaction of ferritin with H2O2. When linoleic acid micelles or phosphatidyl choline liposomes were incubated with ferritin and H2O2, lipid peroxidation increased in the presence of ferritin and H2O2 in a concentration-dependent manner. The hydroxyl radical scavengers, azide and thiourea, prevented lipid peroxidation induced by the ferritin/H2O2 system. The iron specific chelator desferoxamine also prevented ferritin/H2O2 systemmediated lipid peroxidation. These results demonstrate the possible role of iron in ferritin/H2O2 system-mediated lipid peroxidation. Carnosine is involved in many cellular defense processes, including free radical detoxification. In this study, carnosine, homocarnosine, and anserine were shown to significantly prevent ferritin/H2O2 system-mediated lipid peroxidation and also inhibited the free radical-generation activity of ferritin. These results indicated that carnosine and related compounds may prevent ferritin/H2O2 system-mediated lipid peroxidation via free radical scavenging. [BMB reports 2010; 43(3): 219-224]

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“…High concentrations of carnosine and anserine have been found in the brain and muscles, especially for breast muscle, of mammalian and avian species (Kohen et al, 1988;O'Dowd et al, 1988;Biffo et al, 1990). Recently, carnosine has gained increasing attention as a functional ingredient for human food because of its high antioxidant activity (Mozdzan et al, 2005), high buffering capacity to maintain intracellular pH change (Abe, 2000), and anti-glycating and anti-aldehyde effects (Aldini et al, 2005;Guiotto et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Effects of Dietary Supplementation of Histidine, β-Alanine, Magnesium Oxide, and Blood Meal on Carnosine and Anserine Concentrations of Broiler Breast Meat

et al. 2013

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An experiment was conducted to evaluate the effect of dietary supplementation of histidine, β-alanine, blood meal (BM), magnesium oxide (MgO), and their combination on carnosine and anserine concentrations of broiler breast meat. A total of 210 1-d-old broiler chicks were randomly allotted to 3 replicates of 7 dietary treatments: (1) Control diet (C), (2) C＋histidine (His), (3) C＋β-alanine (β-Ala), (4) C＋histidine＋β-alanine (His＋β-Ala ); (5) C ＋histidine＋β-alanine＋MgO (His＋β-Ala＋MgO), (6) C＋5% BM (BM), and (7) C＋5% BM＋MgO (BM＋ MgO). Histidine, β-alanine, and MgO were supplemented to diets at 2.9, 3.7, and 4.0 g/kg diet, respectively. The broilers were fed experimental diets for 32 d and the concentrations of carnosine and anserine in breast meat were measured weekly and at 32 d of the age. Results indicated that the concentrations of carnosine, anserine, and their sum in breast meat were increased (linear, P＜0.01) with age in all treatments. There were significant differences in carnosine concentrations among treatments in all weeks. At 32 d, all 3 histidine-supplemented treatments (His, His＋ β-Ala, and His＋β-Ala＋MgO) and BM＋MgO treatment had greater (P＜0.05) carnosine concentrations than the control. The anserine concentrations differed (P＜0.01) among treatments at 7, 14, and 21 d. The sum of carnosine and anserine concentrations was the greatest for His＋β-Ala＋MgO treatment, but the least for β-Ala treatment at 21 d. In conclusion, dietary supplementation of histidine alone or with β-alanine may increase carnosine concentrations, but not anserine concentrations of broiler breast meat. Dietary supplementation of additional MgO in diets containing His, β-Ala, and/or BM has little effect on carnosine and anserine concentrations in broiler breast meat.

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Detection, characterisation, and quantification of carnosine and other histidyl derivatives in cardiac and skeletal muscle

Cited by 112 publications

References 31 publications

Calcium sensitizing action of carnosine and other endogenous imidazoles in chemically skinned striated muscle.

Calcium sensitizing action of carnosine and other endogenous imidazoles in chemically skinned striated muscle.

Reaction of ferritin with hydrogen peroxide induces lipid peroxidation

Effects of Dietary Supplementation of Histidine, β-Alanine, Magnesium Oxide, and Blood Meal on Carnosine and Anserine Concentrations of Broiler Breast Meat

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