DETECTION FOR MECA GENE IN EGYPTIAN CLINICAL Staphylococcus aureus SAMPLES AND STUDY THEIR GENOTOXIC ACTIVITY

Abstract

Staphylococcus aureus isolates were isolated from fifty clinical samples collected from Ain Shams University Hospitals (March-July 2016) and identified using biochemical and microbiological tests. PCR was performed using specific primers to determine the isolates of Methicillin-Resistant Staphylococcus aureus (MRSA) based on the presence of mecA gene. Thirty-four isolates from total fifty isolates (68%) were identified as MRSA isolates. To study the genotoxicity for this isolates, forty-five mice were injected with these MRSA isolates and comet and micronuclei assays were performed on mice liver tissues and bone marrow respectively. These assays revealed 24%-22% DNA damage as an indicator for chromosomal breakage by comet and micronucleus assays respectively which indicate that infection with these isolates leads to mutations. Studying these isolate furthermore will give an insight on how critical maintaining high standard hygiene in Egyptian hospitals and attention to infection control system can prevent occur of outbreaks

Bacterial Profile and Antibiotics Susceptibility Pattern of Pleural Effusion Isolates from Sohag University Hospital

“…In our study, mecA gene was detected in 40 % of Staphylococcus aureus isolates; these results are near to the results reported by Hassan et al 21 who reported prevalence of MRSA in cultures from tropics (73%), sub-tropics (39%) and temperate (54%) regions , on Controversy to our results were less than those reported by Anand et al 22 who performed PCR for amplification of the mecA gene in Fifty strains of S. aureus and showed that 32 isolates were mecA gene positive with 64% MRSA prevalence rate, and also less than those reported by Marwa et al 23 in 2019 (MRSA prevalence was (68%).…”