Detection improvement of gliomas in hyperspectral imaging of protoporphyrin IX fluorescence – in vitro comparison of visual identification and machine thresholds

Lehtonen, Samu J.R.; Vrzáková, Hana; Paterno, Jussi J.; Puustinen, Sami; Bednarik, Roman; Hauta-Kasari, Markku; Haneishi, Hideaki; Immonen, Arto; Jääskeläinen, Juha E.; Kämäräinen, Olli‐Pekka; Elomaa, Antti-Pekka

doi:10.1016/j.ctarc.2022.100615

Cited by 3 publications

(2 citation statements)

References 44 publications

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“…The HSC-2 (Senop Oy, Kangasala, Finland) (Figure 2) is a hyperspectral device with snapshot approach and a VIS/NIR spectral range already used in research activities, e.g., the acquisition of high-resolution hyperspectral images acquired in flight by a UAV platform [29,37], the assessments of plant health status through the detection of potassium concentration on leaves [38], and the development of a pathological tissue database in biomedical applications [39,40]. The HSC-2 is a frame-based digital hyperspectral camera equipped with a true global snapshot sensor that can collect the entire 3-dimensional data cube in a single integration period (1).…”

Section: Hyperspectral Correction Methodologymentioning

confidence: 99%

A Novel Correction Methodology to Improve the Performance of a Low-Cost Hyperspectral Portable Snapshot Camera

Genangeli,

Avola,

Bindi

et al. 2023

Sensors

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The development of spectral sensors (SSs) capable of retrieving spectral information have opened new opportunities to improve several environmental and agricultural practices, e.g., crop breeding, plant phenotyping, land use monitoring, and crop classification. The SSs are classified as multispectral and hyperspectral (HS) based on the number of the spectral bands resolved and sampled during data acquisition. Large-scale applications of the HS remain limited due to the cost of this type of technology and the technical difficulties in hyperspectral data processing. Low-cost portable hyperspectral cameras (PHCs) have been progressively developed; however, critical aspects associated with data acquisition and processing, such as the presence of spectral discontinuities, signal jumps, and a high level of background noise, were reported. The aim of this work was to analyze and improve the hyperspectral output of a PHC Senop HSC-2 device by developing a general use methodology. Several signal gaps were identified as falls and jumps across the spectral signatures near 513, 650, and 930 nm, while the dark current signal magnitude and variability associated with instrumental noise showed an increasing trend over time. A data correction pipeline was successfully developed and tested, leading to 99% and 74% reductions in radiance signal jumps identified at 650 and 830 nm, respectively, while the impact of noise on the acquired signal was assessed to be in the range of 10% to 15%. The developed methodology can be effectively applied to other low-cost hyperspectral cameras.

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Section: Hyperspectral Correction Methodologymentioning

confidence: 99%

A Novel Correction Methodology to Improve the Performance of a Low-Cost Hyperspectral Portable Snapshot Camera

Genangeli,

Avola,

Bindi

et al. 2023

Sensors

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“…PpIX solutions were first prepared by dissolving protoporphyrin IX disodium salt in methanol. 48,49 PpIX disodium salt and methanol were procured from Sigma Aldrich (Castle Hill, NSW 2154). To avoid induced photobleaching and improve stability, PpIX solutions were kept in the dark at 5 degrees Celsius and used within seven days of preparation.…”

Section: Ex-vivo Animal Brain Tissuementioning

confidence: 99%

Miniature fluorescence sensor for quantitative detection of brain tumour

Ndabakuranye,

Belcourt,

Sharma

et al. 2024

Lab Chip

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A Phase 2 Sensitivity and Selectivity Study of High-Dose 5-Aminolevulinic Acid in Adult Patients Undergoing Resection of a Newly Diagnosed or Recurrent Glioblastoma

Cozzens,

Lokaitis,

Delfino

et al. 2024

Operative Neurosurgery

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BACKGROUND AND OBJECTIVES: The utility of oral 5-aminolevulinic acid (5-ALA)/protoporphyrin fluorescence for the resection of high-grade gliomas is well documented, but the problem of false-negative observations remains. This study compares high-grade glioma visualization with low/standard dose 5-ALA (<30 mg/kg) to high-dose 5-ALA (>40 mg/kg) to see if by using this higher dose, it is possible to reduce the rate of false-negative observations without increasing the rate of false-positive (FP) observations and therefore increase the sensitivity. METHODS: This is a prospective study of consecutive patients with radiological evidence of presumed high-grade glioma. We reviewed the data from patients who received preoperative low/standard doses and patients who received a preoperative high dose of 5-ALA. Adverse events, dose to observation time, intensity of tumor fluorescence, and results of biopsies in areas of tumor and tumor bed under deep blue light were recorded. RESULTS: A total of 22 patients with high-grade glioma received a dose >40 mg/kg (high-dose) and 9 patients received <30 mg/kg (low/standard dose). There were no serious adverse events related to 5-ALA in any subject. There was a very high sensitivity and specificity of 5-ALA for the presence of tumor in both groups. There were no FP observations (fluorescence with no tumor) in either group. The specificity and the positive predictive value were 100% in both groups. The sensitivity and the negative predictive value were 53.3% and 30.0% in the low/standard dose group and 59.5% and 31.8% in the high-dose group, respectively. CONCLUSION: High-dose oral 5-aminolevulinic/protoporphyrin fluorescence is a safe and effective aid to the intraoperative detection of high-grade gliomas with high sensitivity and specificity. False-negative observations with a high dose do not seem to be less than that with a low/standard dose. The rate of FP observations with both groups remains very low.

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Detection improvement of gliomas in hyperspectral imaging of protoporphyrin IX fluorescence – in vitro comparison of visual identification and machine thresholds

Cited by 3 publications

References 44 publications

A Novel Correction Methodology to Improve the Performance of a Low-Cost Hyperspectral Portable Snapshot Camera

A Novel Correction Methodology to Improve the Performance of a Low-Cost Hyperspectral Portable Snapshot Camera

Miniature fluorescence sensor for quantitative detection of brain tumour

A Phase 2 Sensitivity and Selectivity Study of High-Dose 5-Aminolevulinic Acid in Adult Patients Undergoing Resection of a Newly Diagnosed or Recurrent Glioblastoma

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