Detection In Vitro and Quantitative Estimation of Artificial Microterritories Which Promote Osteogenic Differentiation and Maturation of Stromal Stem Cells

Abstract

Extracellular matrix can regulate multipotent mesenchymal stromal cells (MMSC) differentiation, with potential applications for tissue engineering. A relief of mineralized bone takes essential effect on MMSC fate. Nevertheless, delicate structure and a hierarchy of niches for stromal stem cells and its quantitative parameters are not practically known. Here, we describe the protocol to define the basic approach providing a guiding for in vitro identification of quantitative features of artificial calcium phosphate niches which promotes osteogenic differentiation and maturation of stromal stem cell.

Modulating Effect of Matrices with Calcium Phosphate Coating on Cytotoxicity of Strontium Ranelate and Ibandronic Acid In Vitro

“…After 9-day culturing of myelokaryocytes in the presence of plates with rough CP-coating (3D-matrix) simulating the surface of the mineral part of bone in the process of remodeling [3], the intracellular level of ROS signifi cantly increased (by 133%, p<0.0015; Table 1) in comparison with the 2D culture of the bone marrow cells on plastic. A direct correlation (r S =0.87; p<0.012; n=7) and linear regression (r=0.95; p<0.001; n=7) were observed between ROS level and roughness index Ra of CP-coating.…”

“…Hence, physicochemical properties of CP-materials (relief, calcium and phosphorus ions, etc.) [3] affect cell viability via the enzyme systems controlling intracellular concentration of ROS.…”

“…Design of "cell biochips" carrying artifi cial "niches" of stem cells allows to manage in vitro the behavior mesenchymal stromal cells, and promotes remodeling of the bone-bone marrow system [3] in clinical practice and in experiments.…”

Physical properties and biocompatibility of UHMWPE-derived materials modified by synchrotron radiation

“…Cell monolayer on UHMWPE samples was Giemsa stained by standard protocol. Alkaline phosphatase (ALP) staining of cells adhered to plastic wells was performed as described earlier [20]. Blue sites of enzymatic activity served as cellular ALP staining criteria.…”

Modeling of the mesenchymal stem cell microenvironment as a prospective approach to tissue bioengineering and regenerative medicine (a short review)

“…В ýтом секторе находятся имплантаты с функционализированной поверхностью и биомиметической структурой и (или) поверхностью на основе подражания природному ýкстрацеллюлярному матриксу (ЭЦМ) [7]. В настоящее время наиболее реальным прототипом ЭЦМ для мезенхимных стволовых клеток (МСК) являются кальцийфосфатные (КФ) материалы и покрытия, имитирующие минеральную часть кости [8].…”