Detection Method of Toxoplasma Gondii Tachyzoites

Eassa, Souzan; Bose, Chhanda; Alusta, Pierre; Tarasenko, Olga

doi:10.1063/1.3587472

Cited by 2 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, SCs were used as surface immobilized molecules (ligands) to achieve T. gondii tachyzoites (Tg) recognition. SCs can interact with other species called targets [28]. We assessed eighteen different types of SPCs for their binding affinity toward Tg (targets) using binding assay (result not shown).…”

Section: Resultsmentioning

confidence: 99%

“…gondii RH strain (Tg) was maintained as tachyzoites by serial passage on monolayers of HFFs at 37 0 C with 5% CO 2 in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum, 1% penicillinstreptomycin. The viability of the parasites was determined by trypan blue exclusion [28].…”

Section: B Methods T Gondii Propagation and Purificationmentioning

confidence: 99%

“…Glycoconjugate-polyacrylamide-fluorescein (Glyc-PAA flu) is a synthetic polymer comprised of carbohydrate moieties (Glyc), a polyacrylamide polymer backbone (PAA), and a fluorescein group (flu). Synthetic carbohydrate-bearing moieties (SCs) are suitable ligands for the recognition of pathogens due to the fact that Glyc-PAA-flu can be immobilized in a solid phase and used for binding and recognition [28]. SCs stimulate cell-cell adhesion and subsequent recognition and activation cells' receptors of living organisms [15], [16].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Stimulatory Effect of Synthetic Carbohydrate-Bearing Molecules on Nitric Oxide Production and Enhancing Macrophages Viability upon Exposure of T. Gondii

2016

IJACEBS

View full text Add to dashboard Cite

Toxoplasmosis is a parasitic disease caused by Toxoplasma gondii, an intracellular parasite that affects most types of warm-blooded vertebrate's cells. Individuals at risk for toxoplasmosis include fetuses, newborns, and immunologically impaired patients. Therefore, finding methods for early boosting immune responses cells would help protect against toxoplasmosis. Synthetic carbohydrate bearing moieties (SCs) were used to treat the infected macrophages and to decolonize the tachyzoites. The current research studied the SCs effect on macrophage phagocytosis after 22-24 hours exposure to the Toxoplasma gondii tachyzoites (Tg). Macrophage functional analyses were performed, using nitric oxide (NO) release, upon exposure to Tg treated by SCs. The effect of on macrophage viability (MV) and resistance to T. gondii during phagocytosis were studied using fluorescence-activated cell sorting (FACS). NO release was dependent on the type and the time of SCs exposure to infected macrophages. FACS analysis demonstrated that SCs affected macrophage survival upon T. gondii exposure. Murine macrophages exposed to SC-treated tachyzoites (M+SC+Tg) increase macrophage viability (MV) from 52.6% of macrophages infected with tachyzoites (M+Tg) up to 65%-75%. The present study provides new SCs candidates that can be used in the development of new therapeutics and immunostimulants against T. gondii.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: B Methods T Gondii Propagation and Purificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Stimulatory Effect of Synthetic Carbohydrate-Bearing Molecules on Nitric Oxide Production and Enhancing Macrophages Viability upon Exposure of T. Gondii

2016

IJACEBS

View full text Add to dashboard Cite

show abstract

“…Synthetic Immuno-stimulatory Glycans (SIGs) are suitable ligands for the recognition and binding of pathogens (32). It was reported that SIGs selectively early recognize and bind to Tg (33). Also SIGs stimulate NO production and increase macrophage resistance during acute toxoplasmosis (34).…”

Section: Introductionmentioning

confidence: 99%

Synthetic immunostimulatory glycans interference with host cell apoptosis upon of Toxoplasma gondii infection, in vitro

Eassa¹

2017

IJVS

View full text Add to dashboard Cite

Toxoplasmosis is a protozoan infection of humans and animals caused by Toxoplasma gondii, and it's continuous public health and food safety issue. The tachyzoites (Tg) of T. gondii are the most important stage, as they come in direct contact with immune cells such as a macrophage. Tg can modulate and prevent apoptosis of immune cells while promoting survival of the pathogen. Infections caused by Tg can be eradicated if immune cells could stimulate apoptosis and kill pathogens upon exposure. Apoptosis is characterized by the release of mediators, namely Caspases (Cas). New means are required for inducing apoptosis and enhance immunity in the infected host cell to control toxoplasmosis. The present study investigated whether Synthetic Immuno-stimulatory Glycans (SIGs) influence Cas and Nitric oxide (NO) release and led to Tg damage. Galβ1-3Gal-PAA-fluor (SIG1), Fucα1-4GlcNAcβ-PAA-fluor (SIG2) and GlcNAcβ1-3GalNAcα-PAA-fluor (SIG3) constituted samples studied principally. Murine macrophage had been exposed to the Tg then the SIGs effects on Cas and NO production were determined after 20 hours of pathogen phagocytosis. Here we report that the SIGs had potent in vitro activity against T. gondii; SIG2 was more effective than SIG1 and SIG3, representative by SIG2 treated infected macrophages can induced infected macrophages to release Cas1, 3, and 9. Maximum production of NO by infected macrophages was noticed following the expoxure to all SIGs. Therefore the present study provided the method for the selection of SIGs ligands bearing immunostimulatory factor and apoptotic stimuli properties.

show abstract

Detection Method of Toxoplasma Gondii Tachyzoites

Cited by 2 publications

References 0 publications

Stimulatory Effect of Synthetic Carbohydrate-Bearing Molecules on Nitric Oxide Production and Enhancing Macrophages Viability upon Exposure of T. Gondii

Stimulatory Effect of Synthetic Carbohydrate-Bearing Molecules on Nitric Oxide Production and Enhancing Macrophages Viability upon Exposure of T. Gondii

Synthetic immunostimulatory glycans interference with host cell apoptosis upon of Toxoplasma gondii infection, in vitro

Contact Info

Product

Resources

About