Detection Methods for Foodborne Viruses: Current State-of-Art and Future Perspectives

Abstract

Foodborne viruses have been recognized as important threats to food safety and human health. Rapid and accurate detection is one of the most crucial measures for food safety control. With the development of biology, chemistry, nanoscience, and related interdisciplines, detection strategies have been devised and advanced continuously. This review mainly focuses on the progress of detection methods for foodborne viruses. The current detection methods for foodborne viruses are summarized, including traditional electron microscopy and cultural isolation, immunoassay, molecular technology, biosensors, and newly emerging CRISPR/Cas-based detection technology. Furthermore, a comparison of the detection methods was objectively discussed. This review provides a comprehensive account of foodborne virus detection methods from fundamentals to state-of-the-art and illustrates the advantages and disadvantages of the current methods and proposes the future trends and directions for foodborne virus detection. It is hoped that this review can update current knowledge and present blueprints in order to accelerate futuristic development.

Microfluidic systems for infectious disease diagnostics

“…Very recently, Yin et al discussed the current state-of-art and future perspectives of detection methods for foodborne viruses. 317 Other recent review articles that include microfluidic devices have been worked out, for instance by Gao et al , who discussed advances in microfluidic devices for foodborne pathogen detection, 318 by Shang et al with focus on advances in nanomaterial-based microfluidic platforms, 319 by Ranjbaran et al on microfluidics at the interface of bacteria and fresh produce, 320 or by Quintela et al on advances and limitations of portable and rapid detection technologies for foodborne pathogens. 321 Mi et al summarized microfluidic biosensor tools for foodborne pathogenic bacteria and Su et al investigated microfluidic nucleic acid tests of foodborne viruses.…”

Recent Advances in Crimean-Congo Hemorrhagic Fever Virus Detection, Treatment, and Vaccination: Overview of Current Status and Challenges

“…Implementing biosensors and microfluidics technology has significantly accelerated the development of health monitoring systems by enabling rapid detection methods. Microfluidic technology allows the incorporation of intricate analytical procedures into minute volumes, including but not limited to temperature regulation, sampling, combining, separation, enrichment, and cleansing [228][229][230]. Regarding the early detection of CCHFV infection, novel diagnostic approaches based on biosensors and nano biosensors have received scant consideration.…”

CRISPR/Cas-Powered Amplification-Free Detection of Nucleic Acids: Current State of the Art, Challenges, and Futuristic Perspectives

“…These drawbacks would interfere with the detection results to cause false positives and negatives. Besides, the preamplification step may have an adverse influence on the subsequent reaction steps . In addition, the participation of enzymes increases the cost.…”

“…Besides, the preamplification step may have an adverse influence on the subsequent reaction steps. 29 In addition, the participation of enzymes increases the cost. The preservation of enzymes would be problematic as well.…”