Detection of 16S rRNA gene in round bodies isolated from polyps of rhinosporidiosis

Dhaulakhandi, Dhara B.; Ahluwalia, Kabir; Ravi, A.; Garg, Lalit C.

doi:10.1016/j.meegid.2005.11.002

Cited by 9 publications

(11 citation statements)

References 11 publications

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“…Extending our previous observations, here we report some unique features which have become available due to its growth on a modified culture medium 3 .…”

Section: Introductionsupporting

confidence: 73%

Symmetry patterning by the causative organism of rhinosporidiosis in culture

Dhaulakhandi

Ravi

Garg

et al. 2017

Romanian Journal of Rhinology

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Rhinosporidiosis is a chronic polypoidal infection of the nose, conjunctiva and other sites, believed to be caused by a fungus, Rhinosporidium Seeberi, with a doubtful taxonomy. Polyps contain histological round bodies and the exact mode of infection is not known. The round bodies are filled up with spherules. In tissue the organism forms spherical round bodies approaching 50-500µ in diameter that contain innumerable single-celled organisms that mature at different rates. Mature organisms are approximately 7-9µ in size and escape through a pore that develops in the wall of the round body. The round body does not exist in nature outside the host. The organism in rhinosporidiosis was believed to be uncultivable, until we cultured it for the first time in our laboratory. We further modified the culture medium and succeeded in culturing the causative agent of the disease in CBEML (Cell Biology and Electron Microcopy Laboratory) medium. Here we present some of the peculiar conspicuous features of the organism in culture leading to symmetry patterning.

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“…Extending our previous observations, here we report some unique features which have become available due to its growth on a modified culture medium 3 .…”

Section: Introductionsupporting

confidence: 73%

Symmetry patterning by the causative organism of rhinosporidiosis in culture

Dhaulakhandi

Ravi

Garg

et al. 2017

Romanian Journal of Rhinology

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“…Two palindromic repeats and sequence signature (AATTTTCCG) of cyanobacteria were found in RB's 16S rRNA gene [6]. In this study, multiple sequence alignments showed that sequence signature is shared with 16S rDNA in 11 cyanobacteria and cpDNA in plant Carica papaya, and flanking nucleotides are also similar (Supplementary Figure 3).…”

Section: Resultsmentioning

confidence: 52%

“…Mycology textbooks describe R. seeberi as a fungus of uncertain taxonomy. We used fungal‐specific primers for 18S and 28S rRNA genes, no amplification was seen with DNA from RBs as template, negating fungus in R. seeberi [6]. Presence of chlorophyll led Vanbreuseghem [13] to suggest RB as an alga that was refuted by some authors [3] as chloroplasts have not been seen in RB.…”

Section: Resultsmentioning

confidence: 99%

“…Individual uncontaminated RBs ( R. seeberi ) were isolated from infected tissue (supplementary figure 1) and pure organismal DNA extracted from a single RB (details in [6]). Briefly, between 15‐25 ng DNA extracted from a single RB was checked for purity spectrophotometrically and by running on 0.85% agarose.…”

Section: Methodologiesmentioning

confidence: 99%

“…AJ440719) in purified RBs carefully isolated from infected tissue, and determined the sequence [6]. This study is a detailed analysis of sequence of 16S rRNA gene.…”

Section: Introductionmentioning

confidence: 99%

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Sequence analysis of 16S rRNA gene in Rhinosporidium seeberi shows similarity to plant chloroplast DNA

Ahluwalia¹,

Dhaulakhandi²,

Garg³

2010

Bioinformation

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Identity of causative agent of rhinosporidiosis (Rhinosporidium seeberi) has been controversial since the disease was described in 1900. Extensive sequence alignments and phylogenetic analyses of 16S rRNA gene detected recently by us in R. seeberi , revealed 99% similarity with 16S rDNA in chloroplasts of flowering plants. Study demonstrates R. seeberi is a pigmented prokaryote displaying some characteristics of cyanobacteria, and contains 16S rDNA present in chloroplasts of all groups of land plants. This study and our recent publication of 2006 are the first molecular studies using purified organismal DNA extracted from R. seeberi free of infected tissue.AbbreviationsRB - Round body

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Ear, Nose, and Throat Infections

Johnson¹

2018

Diagnostic Pathology of Infectious Disease

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Detection of 16S rRNA gene in round bodies isolated from polyps of rhinosporidiosis

Cited by 9 publications

References 11 publications

Symmetry patterning by the causative organism of rhinosporidiosis in culture

Symmetry patterning by the causative organism of rhinosporidiosis in culture

Sequence analysis of 16S rRNA gene in Rhinosporidium seeberi shows similarity to plant chloroplast DNA

Ear, Nose, and Throat Infections

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