Detection of 8-oxoguanine and apurinic/apyrimidinic sites using a fluorophore-labeled probe with cell-penetrating ability

Kang, Dong Min; Shin, Jong-Il; Kim, Ji Beom; Lee, Kyung-Ho; Chung, Ji Hyung; Yang, Hye-Won; Kim, Kil-Nam; Han, Ye

doi:10.1186/s12860-019-0236-x

Cited by 5 publications

(2 citation statements)

References 53 publications

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“…The peptide was purified by high-performance liquid chromatography (HPLC) and freezedried after cleavage. The Alexa 488-TaT-S3 peptide was designed for the detection of 8-oxoguanine using a fluorophore-labeled probe with cell-penetrating ability as described previously [44]. The amino acid sequence "GVLRFIMESGAKGCEVVVSG" in the peptide is obtained from human ribosomal protein S3 (hRpS3) that tightly binds 8-oxoG sites.…”

Section: Real-time Qpcr Validationmentioning

confidence: 99%

Canady Helios Cold Plasma Induces Breast Cancer Cell Death by Oxidation of Histone mRNA

Cheng¹,

Murthy²,

Zhuang³

et al. 2021

IJMS

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Breast cancer is the most common cancer among women worldwide. Its molecular receptor marker status and mutational subtypes complicate clinical therapies. Cold atmospheric plasma is a promising adjuvant therapy to selectively combat many cancers, including breast cancer, but not normal tissue; however, the underlying mechanisms remain unexplored. Here, four breast cancer cell lines with different marker status were treated with Canady Helios Cold Plasma™ (CHCP) at various dosages and their differential progress of apoptosis was monitored. Inhibition of cell proliferation, induction of apoptosis, and disruption of the cell cycle were observed. At least 16 histone mRNA types were oxidized and degraded immediately after CHCP treatment by 8-oxoguanine (8-oxoG) modification. The expression of DNA damage response genes was up-regulated 12 h post-treatment, indicating that 8-oxoG modification and degradation of histone mRNA during the early S phase of the cell cycle, rather than DNA damage, is the primary cause of cancer cell death induced by CHCP. Our report demonstrates for the first time that CHCP effectively induces cell death in breast cancer regardless of subtyping, through histone mRNA oxidation and degradation during the early S phase of the cell cycle.

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Section: Real-time Qpcr Validationmentioning

confidence: 99%

Canady Helios Cold Plasma Induces Breast Cancer Cell Death by Oxidation of Histone mRNA

Cheng¹,

Murthy²,

Zhuang³

et al. 2021

IJMS

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“…The guanine oxidation mainly leads to the formation of 8-oxoguanine (8-oxoG), the most studied DNA damage by far, which once persistent in telomeres promotes the abovementioned telomere loss and crisis . The detection of telomeric 8-oxoG may lead to the development of new screening tools able to sense a risk of carcinogenesis at the earliest stage. − …”

Section: Introductionmentioning

confidence: 99%

Luminescent Ruthenium(II) Complexes Used for the Detection of 8-Oxoguanine in the Human Telomeric Sequence

et al. 2023

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Detecting cancer at the early stage of the disease is crucial to keep the best chance for successful treatment. The recent development of genomic screening, a methodology that is addressed to asymptomatic patients presumably at risk of carcinogenesis, has stimulated the quest for new tools able to signal the level of risk. Carcinogenesis has been associated to chronic oxidative stress exceeding the antioxidant defenses and leading to critical genome alteration levels. The telomeric regions are presumably the most exposed to oxidative stress due to their high concentration of guanine (i.e., the easiest oxidizable nucleic base). Accumulation of 8-oxoguanine in telomeres, thus oxidative lesions, was reportedly associated with telomeric crisis and carcinogenesis. In this study, we report on the capacity of Ru(II) polyazaaromatic complexes to photoprobe 8-oxoguanine into the human telomeric sequence with the view of developing new tools for cancer risk screening.

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Development and Application of a Chemical Labeling‐Based Biosensing Assay for Rapid Detection of 8‐Oxoguanine and Its Repair in vitro and in Human Cells

Kong

Zhu

et al. 2022

Chin. J. Chem.

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Living cells are constantly threatened by endogenous and environmental agents that can induce various DNA lesions including 8-oxoguanine (8-oxoG). Increasing evidence has suggested that 8-oxoG is not only a biomarker of oxidative stress, but also a novel epigenetic-like modification involved in transcriptional regulation in mammalian cells. Measurement of DNA damage and repair is useful for both basic research and clinical applications, but current methods for 8-oxoG detection still suffer from some problems such as poor selectivity, time consuming and being expensive. Here, we developed a fast and simple biosensing approach for quantitative analysis of 8-oxoG in DNA, which was based on the selective chemical biotinylation of 8-oxoG in conjunction with biotin-streptavidin enzyme-linked immunosorbent assay. We have also successfully applied this method to achieve efficient detection of the repair activities of DNA glycosylases Fpg and hOGG1 toward 8-oxoG in vitro and in human cells. This newly developed biosensing assay should be generally applicable for rapid detection of 8-oxoG and its repair in other organisms.

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Detection of 8-oxoguanine and apurinic/apyrimidinic sites using a fluorophore-labeled probe with cell-penetrating ability

Cited by 5 publications

References 53 publications

Canady Helios Cold Plasma Induces Breast Cancer Cell Death by Oxidation of Histone mRNA

Canady Helios Cold Plasma Induces Breast Cancer Cell Death by Oxidation of Histone mRNA

Luminescent Ruthenium(II) Complexes Used for the Detection of 8-Oxoguanine in the Human Telomeric Sequence

Development and Application of a Chemical Labeling‐Based Biosensing Assay for Rapid Detection of 8‐Oxoguanine and Its Repair in vitro and in Human Cells

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