Detection of a brown planthopper resistance gene bph4 at the same chromosomal position of Bph3 using two different genetic backgrounds of rice

Abstract

The brown planthopper (BPH) resistance gene bph4 has previously been assigned on the short arm of rice chromosome 6. However, the map position of the gene could not be determined. To detect the bph4 locus, 15 polymorphic simple sequence repeat (SSR) markers covering genetic distance of 0.0-63.4 cM on chromosome 6 were used to survey 15 BPH resistant (R) and susceptible (S) individuals from each of the 95 and 78 F 2 populations derived from crosses of TN1/Babawee and Babawee/KDML105, respectively. One SSR marker, RM586, was associated with the R and S from the F 2 populations. Additional markers surrounding the RM586 locus were examined to define the location of bph4. From the genetic linkage map and QTL analysis of 95 and 78 F 2 individuals, the bph4 locus was mapped at the same chromosomal region of Bph3 between two flanking markers RM589 and RM586. Markers linked to the resistance gene explained 58.8-70.1% of the phenotypic variations and can be used for marker-assisted selection in BPH-resistant breeding programs. In addition, our experiment provides evidence that a recessive gene could behave as a dominant gene under different genetic backgrounds.

Development of near-isogenic lines for BPH25(t) and BPH26(t), which confer resistance to the brown planthopper, Nilaparvata lugens (Stal.) in indica rice 'ADR52'

“…Two BPH-resistance genes (Bph3, derived from 'Rathu Heenati' and 'PTB33' and bph4, derived from 'Babawee') were previously reported on the short arm of chromosome 6 (Jairin et al 2007(Jairin et al , 2010. On the other hand, three BPH-resistance genes (Bph1, derived from the indica cultivar Mudgo; bph2, derived from the indica cultivar ASD7; and Bph9, derived from the indica cultivar Pokkali) have been reported on the long arm of chromosome 12 (Murata et al 2000, Sharma et al 2004.…”

SEGREGASI KETAHANAN POPULASI F2 PADI CIHERANG/ SWARNALATA terhadap WERENG BATANG COKLAT

“…Pada populasi persilangan yang bersegregasi pada generasi awal seperti F 2 tidak dimungkinkan adanya ulangan perlakuan, rumpun tanaman stadium anakan dipisahkan, dan diuji terpisah (Jairin et al, 2010). Metode pengujian yang tidak mematikan tanaman, seperti uji embun madu (honey dew test), dapat diaplikasikan pada stadium bibit berdaun 2-4 yang ditanam pada pot terpisah (Do et al, 2009;Qiu et al, 2011).…”

“…Namun demikian, metode ini masih perlu divalidasi dengan membandingkannya dengan teknik baku penapisan secara massal varietas diferensial pada bak benih sebelum diaplikasikan secara luas. Sebelumnya, teknik pengujian ketahanan tanaman secara individual telah diaplikasikan pada tanaman populasi persilangan generasi awal, tetapi pada stadium anakan sehingga dapat diperoleh ulangan (Jairin et al, 2010) dan pada generasi persilangan yang telah lanjut BC 3 F 6 (Yara et al, 2010).…”

Geographic and Research Center Origins of Rice Resistance to Asian Planthoppers and Leafhoppers: Implications for Rice Breeding and Gene Deployment

“…These varieties, many of which contained the Bph1 or bph2 genes for resistance to N. lugens, were planted throughout Asia [6]; however, the utility of these genes was often short-lived as adapted planthopper populations emerged soon after the varieties were first adopted by farmers [7,10,11]. In response to planthopper adaptation to Bph1 and bph2 resistance, current breeding programs have sought to release new varieties with resistance based on novel (not widely deployed) genes, including Bph3/Bph32 (from Rathu Heenati or PTB33), bph4 (from Babawee), and Bph14 and/or Bph15 (introgressed from Oryza officinalis Well ex Watt) [12][13][14][15]. Breeders have also sought to increase the strength and durability of resistance by pyramiding genes (introgressing two or more resistance loci into a single rice line) using marker-assisted selection [7,16].…”

“…Because breeding programs tend to focus on single genes or single donor varieties [7,[12][13][14], comparative studies with multiple donor varieties using a range of planthopper populations are rare [11,16,22]. Furthermore, breeding programs have focused predominantly on resistance in rice seedlings with little information available on resistance in older plants [7,16].…”