Detection of a common genotype among strains ofTaylorella equigenitalis isolated from thoroughbred horses in Japan between 1994 and 1996

Matsuda, Motoo; Miyazawa, T.; Anzai, Tohru

doi:10.1002/(sici)1521-4028(199905)39:2<127::aid-jobm127>3.0.co;2-q

Cited by 8 publications

(6 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These genotypic and phenotypic data, coupled with epidemiological information, confirmed relationships between strains isolated from horses with positive test results within each CEM incident in the United States and also showed a lack of strain relationships between CEM incidents. This study and previous studies (2,8,9,10,11,12,13,16) have demonstrated that PFGE is valuable in discriminating strains of T. equigenitalis. Isolates from horses with positive test results in U.S. outbreaks and isolates from imported horses with positive test results were tested using PFGE, resulting in 15 distinct genotypes.…”

Section: Discussionmentioning

confidence: 99%

“…Molecular genotyping of T. equigenitalis strains by the use of pulsed-field gel electrophoresis (PFGE) or crossed-field gel electrophoresis (CFGE), a variation of PFGE, has been previously performed on isolates from Europe (8), Japan (11,16), Ireland and the United States (12), Norway (13), and Australia (10) by the use of restriction enzymes ApaI and NotI. In Europe, 46 isolates of T. equigenitalis from six countries were classified into 18 genotypes (8).…”

mentioning

confidence: 99%

“…In Europe, 46 isolates of T. equigenitalis from six countries were classified into 18 genotypes (8). In Japan, testing of 130 strains of T. equigenitalis isolated between 1980 and 1996 identified only a single genotype (11,16). Testing of 18 strains isolated in Ireland identified three distinct genotypes, and tests of 8 U.S. strains indicated four distinct genotypes (12).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Pulsed-Field Gel Electrophoresis Genotyping of Taylorella equigenitalis Isolates Collected in the United States from 1978 to 2010

Aalsburg¹,

Erdman²

2011

J Clin Microbiol

View full text Add to dashboard Cite

Taylorella equigenitalis is the etiologic agent of contagious equine metritis (CEM), a venereal disease of horses. A total of 82 strains of T. equigenitalis isolated in the United States were analyzed by pulsed-field gel electrophoresis (PFGE) after digestion of genomic DNA with restriction enzyme ApaI. Twenty-eight of those strains isolated from horses in the 2009 U.S. outbreak (CEM09) were further analyzed with NotI and NaeI enzymes. When ApaI alone was used for analysis, the 82 isolates clustered into 15 different genotypes that clearly defined groups of horses with known epidemiological connections. The PFGE profiles of the CEM09 isolates were indistinguishable after digestion with ApaI, NotI, and NaeI and did not match those of isolates from previous U.S. outbreaks in 1978 and 2006 or of any other isolate from the National Veterinary Services Laboratories (NVSL) culture library. Coupled with the fact that the CEM09 isolates are epidemiologically related, these results suggest a common source for the outbreak not linked to previous occurrences of CEM in the United States.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Pulsed-Field Gel Electrophoresis Genotyping of Taylorella equigenitalis Isolates Collected in the United States from 1978 to 2010

Aalsburg¹,

Erdman²

2011

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…From 1980 to 1996, Matsuda and Miyazawa performed crossed-field gel electrophoresis of restricted genomic DNAs, after separate digestion with ApaI and NotI, against 130 isolates of T. equigenitalis from Thoroughbred mares and stallions in Japan. They reported that only a single genotype was present among all of the isolates [9,10].…”

mentioning

confidence: 99%

Contagious Equine Metritis Eradicated from Japan

Anzai

Kamada

Niwa

et al. 2012

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. Contagious equine metritis (CEM), a contagious venereal disease of horses, invaded Japan in 1980 and spread in the Thoroughbred population of the Hidaka-Iburi district of Hokkaido. To eradicate CEM, we ran a program aimed at detecting Taylorella equigenitalis, the causal agent, in carrier horses by using the PCR test, followed by culling or treatment. In 2001, the first year of the program, 12,356 Thoroughbred racing stallions and mares were tested and 11 carriers were found. Four, two, one, and one carrier mares were detected in 2002, 2003, 2004, and 2005, respectively

show abstract

“…Since then, although CEM has been eradicated from Thoroughbred populations in the United States, Australia and almost European countries, the disease continues to be a problem among Thoroughbred breeders in Japan, causing considerable economic loss. Despite bacterial isolation testing of all broodmares and stallions in main Thoroughbred breeding areas of Japan every inter-breeding season since 1980, occurrence of CEM has persisted and T. equigenitalis has been isolated every year to date [8,9].…”

mentioning

confidence: 99%

Evaluation of the Field Application of PCR in the Eradication of Contagious Equine Metritis from Japan.

Anzai

Wada

Okuda

et al. 2002

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. The effectiveness of the polymerase chain reaction (PCR) as a field application test for the eradication of contagious equine metritis (CEM) was evaluated. Seven-thousands five-hundred and thirty-four genital swabs were collected from 4,026 Thoroughbred broodmares and stallions in Japan to test "high risk" horses as well as for general surveillance testing from 1998 to 2001. Bac terial isolation as well as PCR testing of original specimens and cultured specimens was performed for detection of Taylorella equigenitalis from genital swabs. As a result, T. equigenitalis was detected in 12 mares and 1 stallion by PCR, although the bacteria were isolated from only 2 of the PCR-positive mares. CEM-infected and carrier horses were treated by a combination of chemotherapy and surgery. Subsequent follow-up testing over a 3-year period did not detect T. equigenitalis. It was demonstrated that PCR testing was more sensitive than isolation as a method for the detection of T. equigenitalis from genital swabs of horses in the field. It was therefore suggested that a combination of PCR testing and treatment were useful measures in the eradication of CEM from Japan.

show abstract

Detection of a common genotype among strains ofTaylorella equigenitalis isolated from thoroughbred horses in Japan between 1994 and 1996

Cited by 8 publications

References 6 publications

Pulsed-Field Gel Electrophoresis Genotyping of Taylorella equigenitalis Isolates Collected in the United States from 1978 to 2010

Pulsed-Field Gel Electrophoresis Genotyping of Taylorella equigenitalis Isolates Collected in the United States from 1978 to 2010

Contagious Equine Metritis Eradicated from Japan

Evaluation of the Field Application of PCR in the Eradication of Contagious Equine Metritis from Japan.

Contact Info

Product

Resources

About