Detection of a new enzyme for stereoselective hydrolysis of linalyl acetate using simple plate assays for the characterization of cloned esterases from Burkholderia gladioli

Antinociceptive effect of extracts and compounds from Hofmeisteria schaffneri

“…The stability of esters 1, 4 and 5 toward pig liver esterase (2 mg suspended in 10 ml of distilled water) was estimated using the procedure described by Schlacher et al (1998). The enzyme activity was qualitatively corroborated by monitoring the production of 4-nitrophenol, yellow, from the colorless 4-nitrophenyl acetate (4-NPA) (Innocenti et al, 2008).…”

A Molecular Mechanism of Enantiorecognition of Tertiary Alcohols by Carboxylesterases

“…[24,25] The only hydrolases which are active towards this class of substrates are characterized by a highly conserved GGG(A)X-motif, [26] which is located in the active site and contributes to the formation of the so-called oxyanion hole. [27] This binding pocket stabilizes the oxyanion in the tetrahedral intermediate formed during the catalytic cycle of ester hydrolysis.…”

Novel bacterial isolates for the resolution of esters of tertiary alcohols

“…With the exception of esterase EP6 (Schlacher et al, 1996), all of the crude enzyme preparations were shown to be inactive, the only notable catalytic activity was observed bacterial cells ¥. In order to investigate any possible enantioselectivity, preparative scale reactions were performed with the more active strains.…”