Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

Corman, Victor M.; Eckerle, Isabella; Bleicker, Tobias; Zaki, Aya; Landt, Olfert; Eschbach-Bludau, Monika; Boheemen, Sander van; Gopal, Robin; Ballhause, Matthias; Bestebroer, Theo M.; Muth, Doreen; Müller, Marcel A.; Drexler, Jan Felix; Zambon, Maria; Osterhaus, Albert D. M. E.; Fouchier, Ron A. M.; Drosten, Christian

doi:10.2807/ese.17.39.20285-en

Cited by 523 publications

(587 citation statements)

References 12 publications

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“…World health organization (WHO) determined that the pathogen is a novel coronavirus with many similarities to SARS virus (Ge et al, 2013). A British scientist released its complete sequence recently (Corman et al, 2012). Three days later, European Union scientists devised the real-time PCR method to detect this novel coronavirus; this test makes rapid diagnosis possible (WHO, 2012).…”

Section: Introductionmentioning

confidence: 99%

A meta-analysis to evaluate the effectiveness of real-time PCR for diagnosing novel coronavirus infections

Lin¹,

Ye²,

Yl³

2015

Genet. Mol. Res.

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ABSTRACT. Novel coronavirus (nCoV) belongs to the Coronaviridae family, which includes the virus that causes SARS, or severe acute respiratory syndrome. However, infection source, transmission route, and host of nCoV have not yet been thoroughly characterized. In some cases, nCoV presented a limited person-to-person transmission. Therefore, early diagnosis of nCoV may be of importance for reducing the spread of disease in public. Methods for nCoV diagnosis involve smear dyeing inspection, culture identification, and real-time PCR detection, all of which are proved highly effective. Here, we performed a meta-analysis to evaluate the effectiveness of real-time PCR for diagnosing nCoV infection. Fifteen articles conformed to the inclusion and exclusion criteria for further meta-analysis on the basis of a wide range of publications searched from databases involving PubMed, EMBASE, Web of Science, Medline, ISI. We analyzed the stability and publication bias as well as examined the heterogeneity inspection of real-time PCR detection in contrast to smear staining and culture identification. The fixed-effect model was adopted in our meta-analysis. Our result demonstrated that the combination of real-time PCR and Real-time PCR for nCoV detection smear diagnostics yielded an odds ratio (OR) = 1.91, 95% confidence interval (CI) = 1.51-2.41, Z = 5.43, P < 0.05, while the combination of real-time PCR and culture identification yielded OR = 2.44, 95%CI = 1.77-3.37, Z = 5.41, P < 0.05. Therefore, we propose real-time PCR as an efficient method that offers an auxiliary support for future nCoV diagnosis.

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Section: Introductionmentioning

confidence: 99%

A meta-analysis to evaluate the effectiveness of real-time PCR for diagnosing novel coronavirus infections

Lin¹,

Ye²,

Yl³

2015

Genet. Mol. Res.

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“…All samples were tested for MERS-CoV by using reverse transcription PCR targeting regions upstream of the envelope gene (6). Respiratory and serum samples from all 7 patients were negative for MERS-CoV.…”

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confidence: 99%

Influenza A and B Viruses but Not MERS-CoV in Hajj Pilgrims, Austria, 2014

Aberle¹,

Popow‐Kraupp²,

Kreidl³

et al. 2015

Emerg. Infect. Dis.

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“…The genetic sequence data indicated that this new coronavirus was a betacoronavirus similar to bat coronaviruses, but not similar to any other coronavirus previously described in humans, including the coronavirus that caused SARS in 2003 [6][7][8]. The available evidence related to this nCoV continued to suggest a zoonotic origin for the virus, and experience with the SARS-CoV also showed that any novel virus that is zoonotic in origin has the potential to efficiently transmit from person to person, especially in healthcare settings, and to cause a global pandemic of severe human illness.…”

Section: Introductionmentioning

confidence: 99%

Highlights and conclusions from the technical consultative meeting on novel coronavirus infection, Cairo, Egypt, 14-16 January 2013

Joseph¹,

Malik²,

Mounts³

et al. 2013

East Mediterr Health J

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The emergence of a novel strain of coronavirus in the Arabian Peninsula raised a global health concern in 2012, partly because the majority of human infections were fatal and partly due to its presumed animal origin. An urgent meeting of scientific and public health experts was convened by WHO in January 2013 in view of the limited knowledge available on the epidemiological and natural history of infection with this novel virus. The meeting reviewed current evidence and identified critical knowledge gaps to improve better understanding of the public health risk associated with the virus so as to improve preparedness and to safeguard and protect global health.

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Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

Cited by 523 publications

References 12 publications

A meta-analysis to evaluate the effectiveness of real-time PCR for diagnosing novel coronavirus infections

A meta-analysis to evaluate the effectiveness of real-time PCR for diagnosing novel coronavirus infections

Influenza A and B Viruses but Not MERS-CoV in Hajj Pilgrims, Austria, 2014

Highlights and conclusions from the technical consultative meeting on novel coronavirus infection, Cairo, Egypt, 14-16 January 2013

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