Detection of a Potential New Bartonella Species “Candidatus Bartonella rondoniensis” in Human Biting Kissing Bugs (Reduviidae; Triatominae)

Laroche, Maureen; Bérenger, Jean-Michel; Mediannikov, Oleg; Raoult, Didier; Parola, Philippe

doi:10.1371/journal.pntd.0005297

Cited by 28 publications

(17 citation statements)

References 40 publications

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“…Francisella in the Gulf Coast tick [75]). Others, like sheep keds (Melophagus ovinus [83]) and a single kissing bug species (Eratyrus mucronatus [84]), were found to carry Bartonella species of an unknown phenotype. In our data, Bartonella was the second most abundant taxa found in every life stage of T. rubida.…”

Section: Origin Of the Microbiome Bacteria: Inheritance Vs Environmentmentioning

confidence: 99%

Ontogeny, species identity and environment dominate microbiome dynamics in wild populations of kissing bugs (Triatominae)

Brown

Rodríguez‐Ruano

Poosakkannu

et al. 2020

Preprint

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BackgroundKissing bugs (Triatominae) are blood-feeding insects best known as the vectors of Trypanosoma cruzi, the causative agent of Chagas' disease. Considering the high epidemiological relevance of these vectors, their biology and bacterial symbiosis remains surprisingly understudied. While previous investigations revealed generally low individual complexity but high among-individual variability of the triatomine microbiomes, any consistent microbiome determinants have not yet been identified across multiple Triatominae species. Methods To obtain a more comprehensive view of triatomine microbiomes, we investigated the host-microbiome relationship of five Triatoma species sampled from white-throated woodrat (Neotoma albigula) nests in multiple locations across the USA. We applied optimized 16S rRNA gene metabarcoding with a novel 18S rRNA gene blocking primer to a set of 170 T. cruzi negative individuals across all six instars. Results Triatomine gut microbiome composition is strongly influenced by three principal factors: ontogeny, species identity, and the environment. The microbiomes are characterised by significant loss in bacterial diversity throughout ontogenetic development. First instars possess the highest bacterial diversity while adult microbiomes are routinely dominated by a single taxon. Primarily, the bacterial genus Dietzia dominates late-stage nymphs and adults of T. rubida, T. protracta, and T. lecticularia, but is not present in the phylogenetically more distant T. gerstaeckeri and T. sanguisuga. Species-specific microbiome composition, particularly pronounced in early instars, is further modulated by locality-specific effects. In addition, pathogenic bacteria of the genus Bartonella, acquired from the vertebrate hosts, are an abundant component of Triatoma microbiomes. ConclusionOur study is the first to demonstrate deterministic patterns in microbiome composition among all life stages and multiple Triatoma species. We hypothesize that triatomine microbiome assemblages are produced by species-and life stage-dependent uptake of environmental bacteria and multiple indirect transmission strategies that promote bacterial transfer between individuals. Altogether, our study highlights the complexity of Triatominae symbiosis with bacteria and warrant further investigation to understand microbiome function in these important vectors.

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Section: Origin Of the Microbiome Bacteria: Inheritance Vs Environmentmentioning

confidence: 99%

Ontogeny, species identity and environment dominate microbiome dynamics in wild populations of kissing bugs (Triatominae)

Brown

Rodríguez‐Ruano

Poosakkannu

et al. 2020

Preprint

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“…B. ancashensis was first reported in 2013 isolated from the blood of 2 patients with Peruvian warts (56,85). Moreover, preliminary data have shown several new Bartonella species to have a close phylogenetic relationship with B. bacilliformis, such as in the case of the recently described "Candidatus Bartonella rondoniensis," which shares between 84 and 91% identity with B. bacilliformis (82). Although there are few studies on these species, B. rochalimae has been described to be present in fleas (86) and to be widely disseminated (87)(88)(89)(90).…”

Section: Bartonellaceaementioning

confidence: 99%

“…Currently, 35 species of Bartonella have been identified (http://www.bacterio.net/index .html) and have international standing in nomenclature, and other species have been proposed, several of which are classified as "Candidatus" species (80). New species of Bartonella continue to be discovered, but frequently only partial genetic data obtained by molecular analysis are available (81)(82)(83). B. rochalimae was reported in 2007 to have caused an Oroya fever-like disease in a traveler returning from Peru (57), and a retrospective study also identified this species in the blood of a Peruvian patient with Carrion's disease (84).…”

Section: Bartonellaceaementioning

confidence: 99%

“…On the other hand, B. ancashensis has been described only in the area of Ancash, and no information about its vectors or reservoirs is currently known. "Candidatus Bartonella rondoniensis" has been described in Chagas illness vector kissing bugs (Eratyrus mucronatus) in French Guiana (82).…”

Section: Bartonellaceaementioning

confidence: 99%

“…No isolate is available to perform further studies. This Bartonella species is closely related to B. bacilliformis and B. ancashensis (82 (97), the Winnie strain (GenBank accession number CP003124.1) has both the Virb4/VirD and Vbh systems (92). r Considered a honey bee symbiont.…”

Section: Bartonellaceaementioning

confidence: 99%

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Carrion's Disease: the Sound of Silence

2018

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Carrion's disease (CD) is a neglected biphasic vector-borne illness related to . It is found in the Andean valleys and is transmitted mainly by members of the genus but also by blood transfusions and from mother to child. The acute phase, Oroya fever, presents severe anemia and fever. The lethality is high in the absence of adequate treatment, despite the organism being susceptible to most antibiotics. Partial immunity is developed after infection by , resulting in high numbers of asymptomatic carriers. Following infection there is the chronic phase, Peruvian warts, involving abnormal proliferation of the endothelial cells. Despite potentially being eradicable, CD has been expanded due to human migration and geographical expansion of the vector. Moreover, studies have demonstrated the risk of the development of antimicrobial resistance. These findings, together with the description of new species producing CD-like infections, the presence of undescribed potential vectors in new areas, the lack of adequate diagnostic tools and knowledge of the immunology and bacterial pathogenesis of CD, and poor international visibility, have led to the risk of increasing the potential expansion of resistant strains which will challenge current treatment schemes as well as the possible appearance of CD in areas where it is not endemic.

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